K2250 Environmental Express 5m

ORNL MICROARRAY HYBRIDIZATION PROTOCOLS

  Direct labeling of total RNA with Cy3 and Cy5: A. MATERIALS RNeasy® Mini Kit (Qiagen; Cat # 74106) SuperScript II RT (200U/µL) (Life Technologies; Cat # 18064-014) QIAquick PCR Purification Kit (Qiagen; Cat # 28106)

Inducible shRNA Lentiviral Vectors

at 25° C for 1 hour. Note:   Extending the incubation time to 18 hours typically yields more colonies. 6) Add 1 µl of the Proteinase K solution to each reaction. Incubate for 10 minutes at 37° C. 7) Proceed to Transforming One Shot®

Endurance Training Protocol and Longitudinal Performance Assays for Drosophila melanogaster

, which can be pushed down to immobilize the flies while on the Power Tower. Sponge is used for immobilization because its adaptive size allows easy placement near the bottom of the vial. The softer sponge material also reduces injuries caused by the more solid Flugs