- 询价
- DIMA BIOTECH/缔码生物
- 武汉
- DMC101149P
- 2025年07月12日
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
Rabbit/Human Fc chimeric IgG1
- 形态：
Liquid
- 保存条件：
Store at 2°C-8°C for 6 months
- 克隆性：
Monoclonal
- 标记物：
PE-conjugated
- 适应物种：
Human
- 保质期：
12个月
- 级别：
仅用于科研
- 库存：
Under Development
- 供应商：
缔码生物（武汉）科技有限公司
- 宿主：
Rabbit
- 靶点：
ANPEP
- 抗体英文名：
PE-conjugated Anti-ANPEP antibody(26H2), IgG1 Chimeric mAb
- 抗体名：
PE-conjugated Anti-ANPEP antibody(26H2), IgG1 Chimeric mAb
- 规格：
100test
| SKU | DMC101149P |
|---|---|
| 产品分类 | Monoclonal Antibodies |
| 规格 | Price: 100 test ￥3880.00 |
| 背景 | Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. This membrane-bound zinc metalloprotease is known to serve as a receptor for the HCoV-229E alphacoronavirus as well as other non-human coronaviruses. This gene has also been shown to promote angiogenesis, tumor growth, and metastasis and defects in this gene are associated with various types of leukemia and lymphoma. [provided by RefSeq, Apr 2020] |
| Clone ID | 26H2 |
| 别名 | APN;CD13;GP150;LAP1;P150;PEPN |
| 应用 | Flow Cyt |
| 形态与组成成分 | Liquid，PBS with 0.05% Proclin300, 1% BSA |
| 纯化方式 | Purified from cell culture supernatant by affinity chromatography |
| 反应种属 | Human |
| 推荐稀释比 | Flow Cyt 1:100 |
| 靶点 | ANPEP |
| Uniprot ID | P15144 |
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文献和实验the amount of antibody compared with other subclasses. In case of IgG1 subclass, add 3.3 M NaCl (192.85 g/L) to crude antibody preparation (serum, tissue culture supernatant, or ascites).Apply Binding buffer again ~ 200 ml.Apply Elution buffer ~ 100 ml.Collect 3 ml
) to the Protein A column. Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10 the amount of antibody compared with other subclasses
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
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