- 询价
- DIMA BIOTECH/缔码生物
- 武汉
- DME100204
- 2025年07月09日
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
Rabbit IgG
- 形态：
Powder
- 保存条件：
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12个月
- 级别：
仅用于科研
- 库存：
In Stock
- 供应商：
缔码生物（武汉）科技有限公司
- 宿主：
Rabbit
- 靶点：
CD73
- 抗体英文名：
Anti-CD73 antibody(DM204); Rabbit mAb
- 抗体名：
Anti-CD73 antibody(DM204); Rabbit mAb
- 规格：
50μg
| SKU | DME100204 |
|---|---|
| 产品分类 | Monoclonal Antibodies |
| 规格 | Price: 10μg ￥690.00 ; 50μg ￥2280.00 ; 100 μg ￥4080.00 ; 500 μg ￥13280.00 |
| 背景 | The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq; Mar 2011] |
| Clone ID | DM204 |
| 别名 | CD73;NT5E;5'-Nucleotidase;5'-NT;NT5;NTE |
| 应用 | ELISA; Flow Cyt |
| 形态与组成成分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 纯化方式 | Purified from cell culture supernatant by affinity chromatography |
| 反应种属 | Human |
| 推荐稀释比 | ELISA 1:5000-10000; Flow Cyt 1:100 |
| 靶点 | CD73 |
| Uniprot ID | P21589 |
Figure 1. Flow cytometry analysis with Anti-CD73 (DM204) on Expi293 cells transfected with human CD73 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Figure 2. Flow cytometry analysis of antigen binding of Rabbit anti-human CD73 mAb(DME100204). (A) DME100204 does not bind to Jurkat cells that do not express CD73. (B) A clear peak shift of DME100204 was seen compared to the control when incubated with CD73-expressing HT55 cells, indicating strong binding of DME100204 to CD73. Antibodies were incubated at 10 μg/mL.
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文献和实验Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
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antibody unconjugated； 货号21337，产品描述：GAPDH Antibody；规格 50 ul / 100 ul； 货号3052，产品描述：Rabbit anti-Human IgG secondary antibody HRP conjugated； 货号300904050，产品描述：One-Hour Western kit，10 ml / bottle x 3；
Immunocytology using the HA epitope
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
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