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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
Rabbit IgG
- 形态:
Powder
- 保存条件:
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12个月
- 级别:
仅用于科研
- 库存:
In Stock
- 供应商:
缔码生物(武汉)科技有限公司
- 宿主:
Rabbit
- 靶点:
ADAM9
- 抗体英文名:
Anti-ADAM9 antibody(DM192); Rabbit mAb
- 抗体名:
Anti-ADAM9 antibody(DM192); Rabbit mAb
- 规格:
50μg
| SKU | DME100192 |
|---|---|
| 产品分类 | Monoclonal Antibodies |
| 规格 | Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00 |
| 背景 | This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins; and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions; including fertilization; muscle development; and neurogenesis. The protein encoded by this gene interacts with SH3 domain-containing proteins; binds mitotic arrest deficient 2 beta protein; and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. Several alternatively spliced transcript variants have been identified for this gene. |
| Clone ID | DM192 |
| 别名 | CORD9; MCMP; MDC9; Mltng |
| 应用 | ELISA; Flow Cyt |
| 形态与组成成分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 纯化方式 | Purified from cell culture supernatant by affinity chromatography |
| 反应种属 | Human |
| 推荐稀释比 | ELISA 1:5000-10000; Flow Cyt 1:100 |
| 靶点 | ADAM9 |
| Uniprot ID | Q13443 |

Figure 1. ADAM9 protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-ADAM9 (DM192) on Expi293 cells transfected with human ADAM9 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).

Figure 2. Flow cytometry analysis of antigen binding of rabbit anti-human ADAM9 mAb(DME100192). (A) DME100192 does not bind to CHO-S cells that do not express ADAM9. (B) A clear peak shift of DME100192 was seen compared to the control when incubated with ADAM9-expressing Hela cells, indicating strong binding of DME100192 to ADAM9. Antibodies were incubated at 5 μg/mL.
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文献和实验the amount of antibody compared with other subclasses. In case of IgG1 subclass, add 3.3 M NaCl (192.85 g/L) to crude antibody preparation (serum, tissue culture supernatant, or ascites).Apply Binding buffer again ~ 200 ml.Apply Elution buffer ~ 100 ml.Collect 3 ml
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
祝贺〖雅裕睿安〗成为Signalway Antibody(SAB)独家
antibody unconjugated; 货号21337,产品描述:GAPDH Antibody;规格 50 ul / 100 ul; 货号3052,产品描述:Rabbit anti-Human IgG secondary antibody HRP conjugated; 货号300904050,产品描述:One-Hour Western kit,10 ml / bottle x 3;
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