- 询价
- DIMA BIOTECH/缔码生物
- 武汉
- DME100174
- 2025年07月13日
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
Rabbit IgG
- 形态：
Powder
- 保存条件：
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12个月
- 级别：
仅用于科研
- 库存：
In Stock
- 供应商：
缔码生物（武汉）科技有限公司
- 宿主：
Rabbit
- 靶点：
ROR2
- 抗体英文名：
Anti-ROR2 antibody(DM174); Rabbit mAb
- 抗体名：
Anti-ROR2 antibody(DM174); Rabbit mAb
- 规格：
50μg
| SKU | DME100174 |
|---|---|
| 产品分类 | Monoclonal Antibodies |
| 规格 | Price: 10μg ￥690.00 ; 50μg ￥2280.00 ; 100 μg ￥4080.00 ; 500 μg ￥13280.00 |
| 背景 | The protein encoded by this gene is a receptor protein tyrosine kinase and type I transmembrane protein that belongs to the ROR subfamily of cell surface receptors. The protein may be involved in the early formation of the chondrocytes and may be required for cartilage and growth plate development. Mutations in this gene can cause brachydactyly type B; a skeletal disorder characterized by hypoplasia:aplasia of distal phalanges and nails. In addition; mutations in this gene can cause the autosomal recessive form of Robinow syndrome; which is characterized by skeletal dysplasia with generalized limb bone shortening; segmental defects of the spine; brachydactyly; and a dysmorphic facial appearance. |
| Clone ID | DM174 |
| 别名 | ROR2;NTRKR2 |
| 应用 | ELISA; Flow Cyt; WB |
| 形态与组成成分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 纯化方式 | Purified from cell culture supernatant by affinity chromatography |
| 反应种属 | Human |
| 推荐稀释比 | ELISA 1:5000-10000; Flow Cyt 1:100; WB 1:1000 |
| 靶点 | ROR2 |
| Uniprot ID | Q01974 |
Figure 1. ROR2 protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-ROR2 (DM174) on Expi293 cells transfected with human ROR2 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).
Figure 2. Flow cytometry analysis of antigen binding of rabbit anti-human ROR2 mAb(DME100174). (A) DME100174 does not bind to Jurkat cells that do not express ROR2. (B) A clear peak shift of DME100174 was seen compared to the control when incubated with ROR2-expressing Raji cells, indicating strong binding of DME100174 to ROR2. Antibodies were incubated at 5 μg/mL.
Figure 3.Anti-ROR2 antibody (SKU# DME100174) at 1/1000 dilution Lane : RAJI(human Burkitt's lymphoma B lymphocyte), whole cell lysate Secondary : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution Predicted band size: 105 kDa Observed band size: 140 kDa
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文献和实验Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
祝贺〖雅裕睿安〗成为Signalway Antibody(SAB)独家
antibody unconjugated； 货号21337，产品描述：GAPDH Antibody；规格 50 ul / 100 ul； 货号3052，产品描述：Rabbit anti-Human IgG secondary antibody HRP conjugated； 货号300904050，产品描述：One-Hour Western kit，10 ml / bottle x 3；
Immunocytology using the HA epitope
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
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