- 询价
- DIMA BIOTECH/缔码生物
- 武汉
- DME100155
- 2025年07月11日
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
Rabbit IgG
- 形态：
Powder
- 保存条件：
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12个月
- 级别：
仅用于科研
- 库存：
In Stock
- 供应商：
缔码生物（武汉）科技有限公司
- 宿主：
Rabbit
- 靶点：
CD171
- 抗体英文名：
Anti-CD171 antibody(DM155); Rabbit mAb
- 抗体名：
Anti-CD171 antibody(DM155); Rabbit mAb
- 规格：
50μg
| SKU | DME100155 |
|---|---|
| 产品分类 | Monoclonal Antibodies |
| 规格 | Price: 10μg ￥690.00 ; 50μg ￥2280.00 ; 100 μg ￥4080.00 ; 500 μg ￥13280.00 |
| 背景 | The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain; consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III); is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development; including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia; retardation; aphasia; spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants; some of which include an alternate exon that is considered to be specific to neurons. |
| Clone ID | DM155 |
| 别名 | CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1 |
| 应用 | ELISA; Flow Cyt |
| 形态与组成成分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 纯化方式 | Purified from cell culture supernatant by affinity chromatography |
| 反应种属 | Human |
| 推荐稀释比 | ELISA 1:5000-10000; Flow Cyt 1:100 |
| 靶点 | CD171 |
| Uniprot ID | P32004 |
Figure 1. ELISA plate pre-coated by 1 μg/ml (100 μl/well) Human CD171 protein, His tagged protein PME100173 can bind Rabbit anti-CD171 monoclonal antibody (clone: DM155) in a linear range of 1-100 ng/ml.
Figure 2. CD171 protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-CD171 (DM155) on Expi293 cells transfected with human CD171 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).
Figure 3. Flow cytometry analysis of antigen binding of rabbit anti-human CD171 mAb(DME100155). (A) DME100155 does not bind to CHO-S cells that do not express CD171. (B) A clear peak shift of DME100155 was seen compared to the control when incubated with CD171-expressing Hela cells, indicating strong binding of DME100155 to CD171. Antibodies were incubated at 5 μg/mL.
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文献和实验T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
al. 2006b ). MATERIALS Reagents Anti-CD24 monoclonal antibody (mAb) (J11d clone) (for Protocol 3) Use either culture supernatant that contains anti-CD24 mAb or purified anti-CD24 mAb (see Step 55). BDPharmLyse (red blood cell lysing
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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