- 询价
- DIMA BIOTECH/缔码生物
- 武汉
- DME100077
- 2025年07月11日
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
Rabbit IgG
- 形态：
Powder
- 保存条件：
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12个月
- 级别：
仅用于科研
- 库存：
In Stock
- 供应商：
缔码生物（武汉）科技有限公司
- 宿主：
Rabbit
- 靶点：
CD33
- 抗体英文名：
Anti-CD33 antibody(DM77); Rabbit mAb
- 抗体名：
Anti-CD33 antibody(DM77); Rabbit mAb
- 规格：
50μg
| SKU | DME100077 |
|---|---|
| 产品分类 | Monoclonal Antibodies |
| 规格 | Price: 10μg ￥690.00 ; 50μg ￥2280.00 ; 100 μg ￥4080.00 ; 500 μg ￥13280.00 |
| 背景 | Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins; two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6:SHP-1 and PTPN11:SHP-2. In turn; these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase:PI3K. |
| Clone ID | DM77 |
| 别名 | CD33;SIGLEC3;gp67 |
| 应用 | ELISA; Flow Cyt |
| 形态与组成成分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 纯化方式 | Purified from cell culture supernatant by affinity chromatography |
| 反应种属 | Human |
| 推荐稀释比 | ELISA 1:5000-10000; Flow Cyt 1:100 |
| 靶点 | CD33 |
| Uniprot ID | P20138 |
Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD33 protein, hFc-His tagged protein PME100039 can bind Rabbit anti-CD33 monoclonal antibody (clone: DM77) in a linear range of 1-100 ng/ml.
Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human CD33 (B) were surface stained with Rabbit anti-CD33 monoclonal antibody 1μg/ml (clone: DM77) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 3. Flow cytometry data of serially titrated Rabbit anti-CD33 monoclonal antibody (clone: DM77) on Expi 293 cell line transfected with human CD33. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 4. Flow cytometry analysis of antigen binding of rabbit anti-human CD33 mAb(DME100077). (A) DME100077 does not bind to 293T cells that do not express CD33. (B) A clear peak shift of DME100077 was seen compared to the control when incubated with CD33-expressing THP-1 cells, indicating strong binding of DME100077 to CD33. Antibodies were incubated at 10 μg/mL.
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文献和实验Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
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【翻译】Development trends for monoclonal antibody cancer therapeutics
7 and patients frequently developed antibodies to the mouse-derived proteins, which limited their utility8, 9. In addition, only certain murine mAb isotypes have been shown to effectively bind to and activate elements of the human immune
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