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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
Rabbit IgG
- 形态:
Powder
- 保存条件:
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12个月
- 级别:
仅用于科研
- 库存:
In Stock
- 供应商:
缔码生物(武汉)科技有限公司
- 宿主:
Rabbit
- 靶点:
CD48
- 抗体英文名:
Anti-CD48 antibody(DM44); Rabbit mAb
- 抗体名:
Anti-CD48 antibody(DM44); Rabbit mAb
- 规格:
50μg
| SKU | DME100043 |
|---|---|
| 产品分类 | Monoclonal Antibodies |
| 规格 | Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00 |
| 背景 | This gene encodes a member of the CD2 subfamily of immunoglobulin-like receptors which includes SLAM (signaling lymphocyte activation molecules) proteins. The encoded protein is found on the surface of lymphocytes and other immune cells; dendritic cells and endothelial cells; and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain; however; but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Clone ID | DM44 |
| 别名 | CD48; BCM1; SLAMF2; BLAST; BLAST1; MEM-102; TCT.1; BCM-1; SLAMF-2; BLAST-1 |
| 应用 | ELISA; Flow Cyt; WB |
| 形态与组成成分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 纯化方式 | Purified from cell culture supernatant by affinity chromatography |
| 反应种属 | Human |
| 推荐稀释比 | ELISA 1:5000-10000; Flow Cyt 1:100; WB 1:1000 |
| 靶点 | CD48 |
| Uniprot ID | P09326 |

Figure 1. Expi 293 cell line transfected with irrelevant protein (left) and human CD48 (right) were surface stained with Rabbit anti-CD48 monoclonal antibody 1μg/ml ( clone: DM44) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Figure 2. Flow cytometry data of serially titrated Rabbit anti-CD48 monoclonal antibody ( clone: DM44) on H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Figure 4. Flow cytometry analysis of antigen binding of rabbit anti-human CD48 mAb(DME100043). (A) DME100043 does not bind to 293T cells that do not express CD48. (B) A clear peak shift of DME100043 was seen compared to the control when incubated with CD48-expressing Raji cells, indicating strong binding of DME100043 to CD48. Antibodies were incubated at 2 μg/mL.

Figure 5. Anti-CD48 antibody (SKU# DME100043) at 1/1000 dilution Lane : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate Secondary : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution Predicted band size: 28 kDa Observed band size: 40 kDa
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文献和实验Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
祝贺〖雅裕睿安〗成为Signalway Antibody(SAB)独家
antibody unconjugated; 货号21337,产品描述:GAPDH Antibody;规格 50 ul / 100 ul; 货号3052,产品描述:Rabbit anti-Human IgG secondary antibody HRP conjugated; 货号300904050,产品描述:One-Hour Western kit,10 ml / bottle x 3;
:使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。(图 A)使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit,抗体 1:100 稀释;(图 B) 采用普通免疫组化试剂盒,抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注:使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK (Erk1/2)Rabbit mAb 对正常小鼠心脏组织进行免疫
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