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CD48抗体/Anti-CD48 antibody(DM44

); Rabbit mAb
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  • 询价
  • DIMA BIOTECH/缔码生物
  • 武汉
  • DME100043
  • 2025年07月11日
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 亚型

      Rabbit IgG

    • 形态

      Powder

    • 保存条件

      Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

    • 克隆性

      Monoclonal

    • 标记物

      Unconjugated

    • 适应物种

      Human

    • 保质期

      12个月

    • 级别

      仅用于科研

    • 库存

      In Stock

    • 供应商

      缔码生物(武汉)科技有限公司

    • 宿主

      Rabbit

    • 靶点

      CD48

    • 抗体英文名

      Anti-CD48 antibody(DM44); Rabbit mAb

    • 抗体名

      Anti-CD48 antibody(DM44); Rabbit mAb

    • 规格

      50μg

    SKU DME100043
    产品分类 Monoclonal Antibodies
    规格 Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00
    背景 This gene encodes a member of the CD2 subfamily of immunoglobulin-like receptors which includes SLAM (signaling lymphocyte activation molecules) proteins. The encoded protein is found on the surface of lymphocytes and other immune cells; dendritic cells and endothelial cells; and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain; however; but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene.
    Clone ID DM44
    别名 CD48; BCM1; SLAMF2; BLAST; BLAST1; MEM-102; TCT.1; BCM-1; SLAMF-2; BLAST-1
    应用 ELISA; Flow Cyt; WB
    形态与组成成分 Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
    纯化方式 Purified from cell culture supernatant by affinity chromatography
    反应种属 Human
    推荐稀释比 ELISA 1:5000-10000; Flow Cyt 1:100; WB 1:1000
    靶点 CD48
    Uniprot ID P09326

    DME100043-CD48-Fig.1-FC-1.jpg

    Figure 1. Expi 293 cell line transfected with irrelevant protein (left) and human CD48 (right) were surface stained with Rabbit anti-CD48 monoclonal antibody 1μg/ml ( clone: DM44) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

    DME100043-CD48-Flow-FIG2.jpg

    Figure 2. Flow cytometry data of serially titrated Rabbit anti-CD48 monoclonal antibody ( clone: DM44) on H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

    dme100043-cd48-fc3.jpg

    Figure 4. Flow cytometry analysis of antigen binding of rabbit anti-human CD48 mAb(DME100043). (A) DME100043 does not bind to 293T cells that do not express CD48. (B) A clear peak shift of DME100043 was seen compared to the control when incubated with CD48-expressing Raji cells, indicating strong binding of DME100043 to CD48. Antibodies were incubated at 2 μg/mL.

    dme100043-cd48-wb1.jpg

    Figure 5. Anti-CD48 antibody (SKU# DME100043) at 1/1000 dilution Lane : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate Secondary : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution Predicted band size: 28 kDa Observed band size: 40 kDa

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    图标文献和实验
    相关实验
    • Purification of mAb (IgG)

        Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers

    • 祝贺〖雅裕睿安〗成为Signalway Antibody(SAB)独家

      antibody unconjugated; 货号21337,产品描述:GAPDH Antibody;规格 50 ul / 100 ul; 货号3052,产品描述:Rabbit anti-Human IgG secondary antibody HRP conjugated; 货号300904050,产品描述:One-Hour Western kit,10 ml / bottle x 3;

    • 组化染色背景高?没信号?一篇文章带你快速掌握免疫组化!

      :使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。(图 A)使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit,抗体 1:100 稀释;(图 B) 采用普通免疫组化试剂盒,抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注:使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK (Erk1/2)Rabbit mAb 对正常小鼠心脏组织进行免疫

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