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- 百欧博伟
- bio-103776
- 国内
- 2026年05月29日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 英文名：
pTRV 2
- 保质期：
3年
- 供应商：
百欧博伟
- 保存条件：
-80
平台编号：bio-103776
启动子：CaMV 35S promoter
复制子：pVS1 oriV，ori
质粒大小：9663bp
原核抗性：Kan
克隆菌株：DH5α
5测序引物：35S:GACGCACAATCCCACTATCC
3测序引物：根据序列设计引物
备注：Tobacco rattle virus RNA2-based VIGS vector
质粒简介： Tobacco rattle virus (TRV) is a bipartite, positive-sense RNA virus. TRV1 (i.e., RNA1) encodes 134 and 194 kDa replicase proteins from the genomic RNA, 29 kDa movement protein, and 16 kDa cysteine-rich protein from subgenomic RNAs. TRV1 can replicate and move systemically without TRV2 (i.e., RNA2). TRV2 encodes coat protein from the genomic and two nonstructural proteins from subgenomic RNAs (A). To develop the TRV-based VIGS vector, a cDNA clone of TRV1 or TRV2 (Ppk20 strain) was placed between a duplicated CaMV 35S promoter (2X35S) and nopaline synthase terminator (NOSt) in an Agrobacterium T-DNA vector. In the TRV2 cDNA construct, the nonessential structural genes were replaced with MCS for cloning the target gene sequences. Further, a self-cleaving ribozyme site was included at the 3' end of TRV2. The TRV1 (AF406990)-based viral vector, pTRV1 (linear plasmid 6.791 kb), is used along with pTRV2 for silencing. The pTRV2 has three variants. The first one is suitable for conventional cloning (AF406991, linear plasmid 9663 nucleotide). The second variant is a gateway-cloning-compatible vector. The third variant is suitable for ligation-independent cloning. Rz, self-cleaving ribozyme; MCS, multiple cloning site; CP, coat protein; MP, movement protein. These vectors were created by Dr. S.P. Dinesh-Kumar, UC Davis, USA. Conventional cloning-compatible (YL156) and ligation-independent-compatible (YY13TRV2) vector plasmid DNA can be obtained from ABRC, Stock No. CD3-1040 vector name YL156. Ligation-independent-compatible TRV2 vector plasmid DNA is ABRC Stock No. CD3-1042, vector name YY13.
说明书下载：pTRV1基本信息
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文献和实验VIGS: A Tool to Study Fruit Development in Solanum lycopersicum
-Induced Gene Silencing (VIGS) of Delila and Rosea1 produces a color change in the silenced area easily identifiable. Del/Ros1 VIGS is achieved by agroinjection of an infective clone of Tobacco Rattle Virus (pTRV1 and pTRV2 binary plasmids) directly
相关专题 his method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research objectives
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