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- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 英文名：
pLV3-U6-shRNA-Negative-Control-Puro
- 保质期：
3个月~12个月
- 供应商：
上海禾午生物有限公司
- 保存条件：
负20℃&负80℃
- 规格：
干粉&甘油菌
| 名称 | pLV3-U6-shRNA-Negative-Control-Puro |
| 别称 | · |
| 质粒宿主 | 哺乳细胞,慢病毒 |
| 质粒用途 | 干扰沉默 |
| 片段类型 | shRNA |
| 片段物种 | 对照 |
| 原核抗性 | Amp |
| 筛选标记 | Puro |
| 启动子 | U6 |
| 复制子 | pUC |
| 感受态 | Stbl3 |
| 温度 | 37度 |
| 正向引物 | pCDH-U6-shRNA-EF1-Puro |
| 反向引物 | U6-F(2023.11.2合成)(发夹结构) |
| pLV3-U6-SLC7A11(mouse)-shRNA1-Neo |
| pLV3-U6-SLC7A11(human)-shRNA1-Neo |
| pLV3-U6-RAB10(human)-shRNA1-Puro |
| pLV3-U6-RAB27A(human)-shRNA1-Puro |
| hU6-MCS-Ubiquitin-EGFP-IRES-puromycin |
| pLV3-U6-RAB31(human)-shRNA1-Puro |
| pLV3-U6-ACTG1(human)-shRNA1-CopGFP-Puro |
| pLV3-U6-POTEE(human)-shRNA1-CopGFP-Puro |
| pLV3-U6-POTEE(human)-shRNA2-CopGFP-Puro |
| pLV3-U6-POTEE(human)-shRNA3-CopGFP-Puro |
| pLV3-U6-Itgbl1(mouse)-shRNA1-CopGFP-Puro |
| pLV3-U6-circ0000511(human)-shRNA1-CopGFP-Puro |
| pLV3-U6-Nsun2(mouse)-shRNA2-CopGFP-Puro |
| pLV3-U6-Nsun2(mouse)-shRNA3-CopGFP-Puro |
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文献和实验. Moreover, to improve the constructing and screening efficiency for the shRNA-expression recombinant clones, these four DNA vectors are further reconstructed by inserting a stuffer of puromycin resistance gene (Puro R ) between restriction enzyme Cla I and Hind III
of controls to ensure the validity of the data. The editors of Nature Cell Biology have recommended several controls (2). Two of these controls are: A negative control siRNA with the same nucleotide composition as your siRNA but which lacks significant
(2). Two of these controls are: A negative control siRNA with the same nucleotide composition as your siRNA but which lacks significant sequence homology to the genome . To design a negative control siRNA, scramble the nucleotide sequence of the gene-specific
技术资料
