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- NM_001042719.2;Mir5131; PA-PLA1
- 上海
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 提供商：
上海博尔森生物科技有限公司
- 服务名称：
pLV3-CMV-Ddhd1(mouse-gc-opt)-3×FLAG-CopGFP-Puro
- 规格：
干粉质粒
pLV3-CMV-Ddhd1(mouse-gc-opt)-3×FLAG-CopGFP-Puro
[ 产品信息 ]
[ 质粒简介 ]
pLV3-CMV-Ddhd1(mouse-gc-opt)-3×FLAG-CopGFP-Puro
[ 质粒图谱 ]
根据质粒序列用软件生成相应图谱
[ 参考序列 ]
acgcgtgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattgccgcattgcagagatattgtatttaagtgcctagctcgatacaataaacgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacctgaaagcgaaagggaaaccagagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccactgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcctcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagattggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtATCGGTtaacttttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttatcgatactagtattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtTtatataagcagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagattctagagctagcCTGATACGAACTCGgaattcGCCACCATGAATTACCCAGGCAGAGGAGCTCCCAGATCTCCAGAAAGGAATGGTAGAGGGGGAGGAGCCTGGGAACTGGGATCTGACGCCGCTGGGGTTTTTGGAGGAGGCGGATGTTGTTTTGAGCACCAGCCCGGAGATTCTGTGCCACTGTCTCTGCTGAGGGCTGAGCCTCTGCATCTCGCTCCTGGAGCCGACGACCTCTCACACCTGGCTCTCGACCCATGCCTGTCTGACGAAACATACGATTTCAGTAGCGCAGAGTCTGGTTCTAGTCTCAGATATTACAGTGAGGGGGAAAGTGCAGGAGGCGGAAGTAGCAGCAGCCCACCACCACCATTGGTAGCTCAAAACAGTGGAGGAGGAGGTGCAGCTGGAGGGGGACCAGGTGATAGGAAAAGAGCAAGACCAGGAGGCGCAGCCGCTAGGCATAGATACGAGGTAGTGACAGAACTGGGGCCTGAAGAGGTGAGGTGGTTTTACAAGGAGGATAAAAAGACCTGGAAACCATTCATTGGCTATGATTCCTTGAGGATCGAGCTGGCCTTTAGGACACTGCTTCAGACTACTGGAGCTCAAGCCCGAGCTGCTGGACGGGATGGTGATAGGGTCTGTGGGCCCTCTTCCTCCTTTGGGGAGGAGGACGAGGAGGACAGCGCATGTGGTTTCTGTCCTAGGGCTGCCGGGCCTGAACCAGAGATGGAGGAGTTGGTTACAATCGAGCCCGTGTGTGTCAGAGGGGGCCTGTACGAGGTGGATGTGACCCAGGGTGAGTGCTATCCGGTTTACTGGAATCAGGCTGACAAGATACCCGTGATGAGGGGACAGTGGTTCATAGATGGGACATGGCAGCCTCTGGAAGAGGAGGAGTCCAACCTGATTGAGCAGGAACATCTCTCCTGCTTTAGAGGGCAGCAGATGCAGGAGAACTTCGATATTGAGGTCAGCAAGAGCCTGGACGGCAAAGATgttgtctaccacctccctcccttctccctcccttctctgttcaaatggagaggatataaggagagcacagatgccacaggtcttaagcgaaagcgttcccaagctgttcacagtttcaagctgagtcggaaccacgtggactggcacagcatggatgaagtctatctctacagtgacgcaacaacatccaaaattgcgagaaccgttacccaaaaactgggcttttctaaagcctccagcagtgggaccagactgcacagaggttatgtagaagaagccacactggaagacaagccgtcgcagacttctcatatcgtgtttgttgtgcacggcattggacagaagatggaccaaggaagaatcatcaaaaacacagccatgatgagagaggctgcaaggaaaatggaagaaaagcatttttccaaccatgcaacacatgttgaatttctgcctgttgaatggcgatcaaagcttactcttgatggagacacagttgattccatcactcctgacaaagtgcgaggcctgagggacatgctcaacagcagcgcgatggacatcatgtactacaccagcccactctacagggatgagctagttaaaggccttcagcaagagctgaatcgattatattcccttttctgttcccggaatccagactttgaagagaaagggggtaaagtctcaatagtgtcccattccttggggtgtgtaatcacttatgacatcatgatgggctggaatccaggtggactctatgagcagctgctgcagaaggaggaagagctgcccgacgagcggtggatgagctatgaagagcggcatcttcttgacgagctctatataacaaagcgacggctgagggaaatagaagatcggctgcacggattgaaggcgccatccatatcacaaacacctgccttaaaatttaaggttgaaaatttcttctgtatgggatctccgctagcagtttttttggcattacgcggcatccgaccaggaaattctggaagtcaagaccacattttacctagagagatctgtaaccgcttactaaatatttttcatcctacggacccagtggcctacagattagaaccgttgattttgaaacactacagcaacatttctccagtccagatccactggtacaacacttcaaaccctctaccttatgagcatatgaagccaaattttctcaacccagcaaaagaacctacctcagtttcagacagtgagaacatagcagctatcccaagccctgtgacctcccccgtcttatctcggcgccactatggagaatctataactaacattggcaaagcaagcatattgggggctgctagcattggaaagggccttggaggaatgttgttctcaagatttggacgttcttcagcatcacagccgtctgagccatctaaagactcactggaagatgataagaagccttcagcttcaccctctaccaccaccgtggcaacgcagaccctgccgcacagtggctccggcttcctcgactccgcatatttcagacttcaagaatcgttcttttatctcccacaacttctttttccggaaaatgtaatgcagagtaaagatgatagcctcgtggaactggaacacaggatcgactttgaactcagagaaggccttgtggagagccgctattggtcagccgtcacgtcgcacactgcctattggtcatccttggatgtcgctctttttcttttaacattcatgtacaaacacgagcatgataccgaggccaagcccagcctaggttcactcggatccGACTACAAAGACCATGACGGTGATTATAAAGATCATGACATCGACTACAAGGATGACGATGACAAGtagcggccgcGaaggatctgcgatcgctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaacgggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacagctgaagcttcgaggggctcgcatctctccttcacgcgcccgccgccctacctgaggccgccatccacgccggttgagtcgcgttctgccgcctcccgcctgtggtgcctcctgaactgcgtccgccgtctaggtaagtttaaagctcaggtcgagaccgggcctttgtccggcgctcccttggagcctacctagactcagccggctctccacgctttgcctgaccctgcttgctcaactctacgtctttgtttcgttttctgttctgcgccgttacagatccaagctgtgaccggcgcctacgctagacgccaccatggagagcgacgagagcggcctgcccgccatggagatcgagtgccgcatcaccggcaccctgaacggcgtggagttcgagctggtgggcggcggagagggcacccccaagcagggccgcatgaccaacaagatgaagagcaccaaaggcgccctgaccttcagcccctacctgctgagccacgtgatgggctacggcttctaccacttcggcacctaccccagcggctacgagaaccccttcctgcacgccatcaacaacggcggctacaccaacacccgcatcgagaagtacgaggacggcggcgtgctgcacgtgagcttcagctaccgctacgaggccggccgcgtgatcggcgacttcaaggtggtgggcaccggcttccccgaggacagcgtgatcttcaccgacaagatcatccgcagcaacgccaccgtggagcacctgcaccccatgggcgataacgtgctggtgggcagcttcgcccgcaccttcagcctgcgcgacggcggctactacagcttcgtggtggacagccacatgcacttcaagagcgccatccaccccagcatcctgcagaacgggggccccatgttcgccttccgccgcgtggaggagctgcacagcaacaccgagctgggcatcgtggagtaccagcacgccttcaagacccccatcgccttcgccagatcccgcgctcagtcgtccaattctgccgtggacggcaccgccggacccggctccaccggatctcgcgagggcagaggaagtcttctaacatgcggtgacgtggaggagaatcccggccctatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgaaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaATCATCGTCCTTTCCTtggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtctTcgccttcgccctcagacgagtcggatctccctttggGccgcctccccgcctggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaacgaaGataagatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggctctagctaTCCCGCCCCTAACTCCGCCCAtcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctagacttttgcagagaccaaattcgtaatcatgtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgccaagctg
展开一键复制序列（有部分质粒是非全长序列)
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[ 产品信息 ]
| 货号: | BES251721PVE |
|---|---|
| 名称: | pLV3-CMV-Ddhd1(mouse-gc-opt)-3×FLAG-CopGFP-Puro |
| 别称: | |
| 基因长: | 2757bp |
| 规格: | |
| 质粒宿主： | 哺乳细胞,慢病毒 |
| 质粒用途： | 蛋白表达 |
| 片段类型： | ORF |
| 片段物种： | |
| 原核抗性： | Amp |
| 筛选标记： | Puro |
| 荧光标记： | 绿色 |
| 启动子: | CMV |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | Stbl3 |
| 温度: | 37度 |
pLV3-CMV-Ddhd1(mouse-gc-opt)-3×FLAG-CopGFP-Puro
[ 质粒图谱 ]
根据质粒序列用软件生成相应图谱
[ 参考序列 ]
acgcgtgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattgccgcattgcagagatattgtatttaagtgcctagctcgatacaataaacgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacctgaaagcgaaagggaaaccagagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccactgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcctcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagattggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtATCGGTtaacttttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttatcgatactagtattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtTtatataagcagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagattctagagctagcCTGATACGAACTCGgaattcGCCACCATGAATTACCCAGGCAGAGGAGCTCCCAGATCTCCAGAAAGGAATGGTAGAGGGGGAGGAGCCTGGGAACTGGGATCTGACGCCGCTGGGGTTTTTGGAGGAGGCGGATGTTGTTTTGAGCACCAGCCCGGAGATTCTGTGCCACTGTCTCTGCTGAGGGCTGAGCCTCTGCATCTCGCTCCTGGAGCCGACGACCTCTCACACCTGGCTCTCGACCCATGCCTGTCTGACGAAACATACGATTTCAGTAGCGCAGAGTCTGGTTCTAGTCTCAGATATTACAGTGAGGGGGAAAGTGCAGGAGGCGGAAGTAGCAGCAGCCCACCACCACCATTGGTAGCTCAAAACAGTGGAGGAGGAGGTGCAGCTGGAGGGGGACCAGGTGATAGGAAAAGAGCAAGACCAGGAGGCGCAGCCGCTAGGCATAGATACGAGGTAGTGACAGAACTGGGGCCTGAAGAGGTGAGGTGGTTTTACAAGGAGGATAAAAAGACCTGGAAACCATTCATTGGCTATGATTCCTTGAGGATCGAGCTGGCCTTTAGGACACTGCTTCAGACTACTGGAGCTCAAGCCCGAGCTGCTGGACGGGATGGTGATAGGGTCTGTGGGCCCTCTTCCTCCTTTGGGGAGGAGGACGAGGAGGACAGCGCATGTGGTTTCTGTCCTAGGGCTGCCGGGCCTGAACCAGAGATGGAGGAGTTGGTTACAATCGAGCCCGTGTGTGTCAGAGGGGGCCTGTACGAGGTGGATGTGACCCAGGGTGAGTGCTATCCGGTTTACTGGAATCAGGCTGACAAGATACCCGTGATGAGGGGACAGTGGTTCATAGATGGGACATGGCAGCCTCTGGAAGAGGAGGAGTCCAACCTGATTGAGCAGGAACATCTCTCCTGCTTTAGAGGGCAGCAGATGCAGGAGAACTTCGATATTGAGGTCAGCAAGAGCCTGGACGGCAAAGATgttgtctaccacctccctcccttctccctcccttctctgttcaaatggagaggatataaggagagcacagatgccacaggtcttaagcgaaagcgttcccaagctgttcacagtttcaagctgagtcggaaccacgtggactggcacagcatggatgaagtctatctctacagtgacgcaacaacatccaaaattgcgagaaccgttacccaaaaactgggcttttctaaagcctccagcagtgggaccagactgcacagaggttatgtagaagaagccacactggaagacaagccgtcgcagacttctcatatcgtgtttgttgtgcacggcattggacagaagatggaccaaggaagaatcatcaaaaacacagccatgatgagagaggctgcaaggaaaatggaagaaaagcatttttccaaccatgcaacacatgttgaatttctgcctgttgaatggcgatcaaagcttactcttgatggagacacagttgattccatcactcctgacaaagtgcgaggcctgagggacatgctcaacagcagcgcgatggacatcatgtactacaccagcccactctacagggatgagctagttaaaggccttcagcaagagctgaatcgattatattcccttttctgttcccggaatccagactttgaagagaaagggggtaaagtctcaatagtgtcccattccttggggtgtgtaatcacttatgacatcatgatgggctggaatccaggtggactctatgagcagctgctgcagaaggaggaagagctgcccgacgagcggtggatgagctatgaagagcggcatcttcttgacgagctctatataacaaagcgacggctgagggaaatagaagatcggctgcacggattgaaggcgccatccatatcacaaacacctgccttaaaatttaaggttgaaaatttcttctgtatgggatctccgctagcagtttttttggcattacgcggcatccgaccaggaaattctggaagtcaagaccacattttacctagagagatctgtaaccgcttactaaatatttttcatcctacggacccagtggcctacagattagaaccgttgattttgaaacactacagcaacatttctccagtccagatccactggtacaacacttcaaaccctctaccttatgagcatatgaagccaaattttctcaacccagcaaaagaacctacctcagtttcagacagtgagaacatagcagctatcccaagccctgtgacctcccccgtcttatctcggcgccactatggagaatctataactaacattggcaaagcaagcatattgggggctgctagcattggaaagggccttggaggaatgttgttctcaagatttggacgttcttcagcatcacagccgtctgagccatctaaagactcactggaagatgataagaagccttcagcttcaccctctaccaccaccgtggcaacgcagaccctgccgcacagtggctccggcttcctcgactccgcatatttcagacttcaagaatcgttcttttatctcccacaacttctttttccggaaaatgtaatgcagagtaaagatgatagcctcgtggaactggaacacaggatcgactttgaactcagagaaggccttgtggagagccgctattggtcagccgtcacgtcgcacactgcctattggtcatccttggatgtcgctctttttcttttaacattcatgtacaaacacgagcatgataccgaggccaagcccagcctaggttcactcggatccGACTACAAAGACCATGACGGTGATTATAAAGATCATGACATCGACTACAAGGATGACGATGACAAGtagcggccgcGaaggatctgcgatcgctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaacgggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacagctgaagcttcgaggggctcgcatctctccttcacgcgcccgccgccctacctgaggccgccatccacgccggttgagtcgcgttctgccgcctcccgcctgtggtgcctcctgaactgcgtccgccgtctaggtaagtttaaagctcaggtcgagaccgggcctttgtccggcgctcccttggagcctacctagactcagccggctctccacgctttgcctgaccctgcttgctcaactctacgtctttgtttcgttttctgttctgcgccgttacagatccaagctgtgaccggcgcctacgctagacgccaccatggagagcgacgagagcggcctgcccgccatggagatcgagtgccgcatcaccggcaccctgaacggcgtggagttcgagctggtgggcggcggagagggcacccccaagcagggccgcatgaccaacaagatgaagagcaccaaaggcgccctgaccttcagcccctacctgctgagccacgtgatgggctacggcttctaccacttcggcacctaccccagcggctacgagaaccccttcctgcacgccatcaacaacggcggctacaccaacacccgcatcgagaagtacgaggacggcggcgtgctgcacgtgagcttcagctaccgctacgaggccggccgcgtgatcggcgacttcaaggtggtgggcaccggcttccccgaggacagcgtgatcttcaccgacaagatcatccgcagcaacgccaccgtggagcacctgcaccccatgggcgataacgtgctggtgggcagcttcgcccgcaccttcagcctgcgcgacggcggctactacagcttcgtggtggacagccacatgcacttcaagagcgccatccaccccagcatcctgcagaacgggggccccatgttcgccttccgccgcgtggaggagctgcacagcaacaccgagctgggcatcgtggagtaccagcacgccttcaagacccccatcgccttcgccagatcccgcgctcagtcgtccaattctgccgtggacggcaccgccggacccggctccaccggatctcgcgagggcagaggaagtcttctaacatgcggtgacgtggaggagaatcccggccctatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgaaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaATCATCGTCCTTTCCTtggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtctTcgccttcgccctcagacgagtcggatctccctttggGccgcctccccgcctggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaacgaaGataagatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggctctagctaTCCCGCCCCTAACTCCGCCCAtcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctagacttttgcagagaccaaattcgtaatcatgtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgccaagctg
展开一键复制序列（有部分质粒是非全长序列)
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文献和实验相关实验
目的：克隆这个片断。 结果： 1, 5 TGTCTGGGTCTTGCACAATGACA 3 3 TCCGTTGTAGGTAGTATTTGGTC 5 rating L ength Tm GC% oD Ta opt sense 88 23 63.5 47.8 32.7 1 Anti-sense 100 23 60.8 43.5 33.6 1 product 82 406 88.2 49.0 55
Genotyping Assay Design for Single Base Extention and FP-TDI Detection
PRIMER_MIN_TM=54 PRIMER_PRODUCT_SIZE_RANGE=80-300 PRIMER_PRODUCT_OPT_SIZE=250 PRIMER_PAIR_WT_PRODUCT_SIZE_LT=.20 PRIMER_PAIR_WT_PRODUCT_SIZE_GT=.20 PRIMER_MIN_GC=20 PRIMER_MAX_GC=50 PRIMER_SALT_CONC=50 PRIMER_SELF
长度可达 20kb，适合于各种 GC 含量的扩增，可用于多种样本的直接 PCR 如植物叶片、全血等。且扩增仅需 30s/kb，甚至于 2kb/s。如此卓越，爱不释手了吧？ 4. 选择直接扩增试剂盒对粗品扩增 vazyme 生产的各种直扩试剂盒，直接对粗品进行扩增，大大减少了实验的工作周期。如小鼠基因型鉴定可以直接使用 One Step Mouse Genotyping Kit，全血扩增可以直接使用 Blood Direct PCR Kit V2。
技术资料需要更多技术资料 索取更多技术资料
pLV3-CMV-Ddhd1(mouse-gc-opt)-3×FLAG-CopGFP-Puro
￥580
