- ￥1080
- AntibodySystem
- FRJ92860
- France
- 2025年10月09日
- FCM, Isotype Control, In vivo Control
- Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Rat IgG1 Isotype Control Antibody (HRPN)
- 抗体英文名：
Rat IgG1 Isotype Control Antibody (HRPN)
- 浓度：
1 mg/ml
- 应用范围：
FCM, Isotype Control, In vivo Control
- 宿主：
Rat
- 保质期：
1 year
- 级别：
科研级
- 库存：
999
- 供应商：
AntibodySystem
- 克隆性：
Monoclonal
- 保存条件：
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
- 亚型：
IgG1
- 规格：
100ug
| 宿主 | Rat |
| 偶联物 | Unconjugated |
| 状态 | Liquid |
| 保存溶液 | 0.01M PBS, pH 7.4. |
| 浓度 | 1 mg/ml |
| 纯度 | >95% as determined by SDS-PAGE. |
| 克隆类型 | Monoclonal |
| 同种型 | IgG1 |
| 应用 | FCM, Isotype Control, In vivo Control |
| 纯化方式 | Protein A/G purified from cell culture supernatant. |
| 内毒素水平 | Please contact with the lab for this information. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| 克隆号 | HRPN |
| 备注 | For research use only. |
AntibodySystem's product range supports research in multiple fields, encompassing 48 types of viruses, superbugs, parasites, oncology, Alzheimer's, Parkinson's, hypersensitivity, immune suppression, and immune activation.
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文献和实验The history of isotype selection for therapeutic antibodies clearly demonstrates that solid understanding of the biologic effects mediated by immunoglobulin (Ig) subclasses IgG1, IgG2, IgG3, and IgG4 and their variants is a prerequisite
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
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