- ￥13200
- AntibodySystem
- 156.25 - 10,000 ng/mL
- 83.4 ng/mL.
- KAH02251
- France
- 2025年07月09日
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- 详细信息
- 技术资料
- 库存：
999
- 供应商：
AntibodySystem
- 检测范围：
156.25 - 10,000 ng/mL
- 检测方法：
Colorimetric
- 应用：
Used for the quantitative determination of Anti-Pembrolizumab Neutralizing Antibody concentration in serum and plasma.
- 标记物：
HRP
- 样本：
Plasma, Serum
- 灵敏度：
83.4 ng/mL.
- 规格：
96T
| 描述 | PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Pembrolizumab has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled Human CD279 antigen and then pipetted into the wells. Anti-Pembrolizumab Neutralizing Antibody in the sample competitively binds to Pembrolizumab with the biotin-labeled Human CD279 antigen. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Anti-Pembrolizumab Neutralizing Antibody bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| 应用 | Used for the quantitative determination of Anti-Pembrolizumab Neutralizing Antibody concentration in serum and plasma. |
| 稳定性和存储 | When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃. |
| 检测方法 | Colorimetric |
| 精准度 |
Intra-Assay Precision (Precision within an assay): <20% Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision. Inter-Assay Precision (Precision between assays): <20% Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision. |
| 样本类型 | Plasma, Serum |
| 实验类型 | Quantitative |
| 灵敏度 | 83.4 ng/mL. |
| 检测范围 | 156.25 - 10,000 ng/mL |
| 回收率 | 80-120% |
| 别名 | MK-3475, CAS: 1374853-91-4 |
| 运输 | 2-8 ℃ |
| 备注 | For Research Use Only. |
AntibodySystem Laboratories SAS was created in 2019 in France as an independent brand, specializing in the research, development, and production of protein and antibody reagents for the life sciences. We are committed to providing high-quality protein and antibody products to life science researchers worldwide. Our offerings include a variety of antibodies and proteins, such as traditional polyclonal antibodies, housekeeping antibodies, tag antibodies, high-activity proteins, functional antibodies for in vivo studies, low-background flow cytometry antibodies, highly specific nanobodies, specialized small molecule antibodies, phospho-specific antibodies, DNA/RNA antibodies, and PEG antibodies—over ten product lines in total.
AntibodySystem products span research areas including 48 viruses, superbugs, parasites, cancer, Alzheimer's, Parkinson's, allergic and hypersensitivity reactions, immune suppression, and immune activation.
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