Anti-Romiplostim (Nplate) ELISA Kit ELISA试剂盒

Anti-Romiplostim (Nplate) ELIS

A Kit ELISA试剂盒
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  • ¥8400
  • AntibodySystem已认证
  • 31.25 - 2,000 ng/mL
  • 17.08 ng/mL
  • KAD76501
  • France
  • 2025年07月12日
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    • 详细信息
    • 技术资料
    • 库存

      999

    • 供应商

      AntibodySystem

    • 检测范围

      31.25 - 2,000 ng/mL

    • 检测方法

      Colorimetric

    • 应用

      Used for the quantitative determination of Anti-Romiplostim concentration in serum and plasma.

    • 标记物

      HRP

    • 样本

      Plasma, Serum

    • 灵敏度

      17.08 ng/mL

    • 规格

      96T

    Anti-Romiplostim (Nplate) ELIS

    描述This assay employs the quantitative sandwich enzyme immunoassay technique. Romiplostim has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and anti-Romiplostim will be captured by immobilized Romiplostim. After washing away any unbound substances, a biotin-labeled Romiplostim is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Romiplostim bound in the initial step. The color development is stopped and the intensity of the color is measured.
    应用Used for the quantitative determination of Anti-Romiplostim concentration in serum and plasma.
    稳定性和存储When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.
    检测方法Colorimetric
    精准度

    Intra-Assay Precision (Precision within an assay): <10%

    Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

    Inter-Assay Precision (Precision between assays): <15%

    Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

     

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    16

    16

    16

    24

    24

    24

    Mean (ng/mL)

    848.7

    206.8

    46.8

    888.7

    225.1

    53.9

    Standard deviation

    24.6

    9.6

    4.3

    34.7

    13.0

    2.8

    CV (%)

    2.9

    4.7

    9.1

    3.9

    5.8

    5.2

    样本类型Plasma, Serum
    实验类型Quantitative
    灵敏度17.08 ng/mL
    检测范围31.25 - 2,000 ng/mL
    回收率80-120%
    运输2-8 ℃
    备注For Research Use Only.

    AntibodySystem Laboratories SAS was established in 2019 in France as an independent brand entity focused on the research, development, and production of protein and antibody reagents in the life sciences field. Our mission is to provide high-quality protein and antibody products to life science researchers worldwide. AntibodySystem's extensive product range includes traditional polyclonal antibodies, classic housekeeping antibodies, tag antibodies, high-activity proteins, in vivo functional antibodies, low-background flow cytometry antibodies, high-specificity nanobodies, specialized small molecule antibodies, premium phospho-specific antibodies, DNA/RNA antibodies, PEG antibodies, and more—spanning over ten product series.
    Currently, AntibodySystem's product lines cover multiple research areas, including 48 types of viruses and superbugs, parasites, tumors, Alzheimer's disease, Parkinson's disease, hypersensitivity and allergic reactions, immune suppression, and immune activation.

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