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- 详细信息
- 技术资料
- 库存:
999
- 供应商:
AntibodySystem
- 检测范围:
0.31-5 μg/mL
- 检测方法:
Colorimetric
- 应用:
Used for the quantitative determination of Adalimumab concentration in serum, plasma and cell culture supernatant.
- 标记物:
HRP
- 样本:
Plasma, Serum
- 灵敏度:
0.156 μg/ml
- 规格:
96T

| 描述 | PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant human TNFa antigen has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled Adalimumab and then pipetted into the wells. Adalimumab in the sample competitively binds to the pre-coated protein with biotin-labeled Adalimumab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Adalimumab bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| 应用 | Used for the quantitative determination of Adalimumab concentration in serum, plasma and cell culture supernatant. |
| 稳定性和存储 | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 20% prior to the expiration date under appropriate storage condition. |
| 检测方法 | Colorimetric |
| 精准度 |
CV<20% |
| 样本类型 | Plasma, Serum |
| 实验类型 | Quantitative |
| 灵敏度 | 0.156 μg/ml |
| 检测范围 | 0.31-5 μg/mL |
| 回收率 | 80-120% |
| 背景 | Adalimumab is a recombinant human IgG1 monoclonal antibody specific for Tumor Necrosis Factor-Alpha (TNF-a) and is used to treat rheumatic arthritis, intestinal disorders, dermatological diseases and cancer. Adalimumab specifically binds to TNF alpha and blocks its interaction with p55 and p75 cell surface TNF receptors and reduces the inflammation and subsequently improves the patient’s health. Drug level quantification can be important to adapt patient prescription or to switch to an alternative TNF inhibitor drug. |
| 别名 | ABP 501, D2E7, Adalimumab, CAS: 331731-18-1 |
| 运输 | 2-8 ℃ |
| 备注 | For Research Use Only. |
Founded in 2019 in France, AntibodySystem Laboratories SAS is a dedicated, independent brand focused on producing high-quality protein and antibody reagents for life science research. We aim to equip researchers worldwide with reliable products for foundational studies in life sciences. AntibodySystem's comprehensive product lineup includes traditional polyclonal antibodies, housekeeping antibodies, tag antibodies, high-performance proteins, in vivo functional antibodies, low-background antibodies for flow cytometry, nanobodies with high specificity, specialized small molecule antibodies, premium phospho-specific antibodies, DNA/RNA antibodies, PEG antibodies, and more.
Our products are designed for various research areas, supporting studies on 48 types of viruses, superbugs, parasites, cancer, Alzheimer's disease, Parkinson's disease, allergic responses, immune suppression, and immune activation.
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