Human IL6 ELISA Kit ELISA试剂盒

Human IL6 ELISA Kit ELISA试剂盒

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  • ¥3280
  • AntibodySystem已认证
  • 4.7 pg/mL - 300 pg/mL
  • 2.37 pg/mL
  • KHC15801
  • France
  • 2025年07月16日
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    • 详细信息
    • 询价记录
    • 技术资料
    • 库存

      999

    • 供应商

      AntibodySystem

    • 检测范围

      4.7 pg/mL - 300 pg/mL

    • 检测方法

      Colorimetric

    • 应用

      Used for the quantitative determination of Human IL6 concentration in serum, plasma and cell culture supernatant.

    • 标记物

      HRP

    • 样本

      Serum, Plasma and Cell culture supernatant.

    • 灵敏度

      2.37 pg/mL

    • 规格

      96T

    Human IL6 ELISA Kit ELISA试剂盒

    描述This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Human IL6 has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Human IL6 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled Human IL6 antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Human IL6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
    应用Used for the quantitative determination of Human IL6 concentration in serum, plasma and cell culture supernatant.
    靶标IL6
    稳定性和存储The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% prior to the expiration date under appropriate storage condition.
    检测方法Colorimetric
    精准度

    Intra-Assay Precision (Precision within an assay): <10%

    Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

    Inter-Assay Precision (Precision between assays): <15%

    Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

     

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    16

    16

    16

    24

    24

    24

    Mean (pg/mL)

    158.0

    36.3

    9.0

    153.5

    34.1

    8.9

    Standard deviation

    13.3

    3.4

    0.5

    16.6

    3.3

    1.0

    CV (%)

    8.4

    9.4

    5.9

    10.8

    9.7

    10.7

     

    样本类型Serum, Plasma and Cell culture supernatant.
    实验类型Quantitative
    灵敏度2.37 pg/mL
    检测范围4.7 pg/mL - 300 pg/mL
    回收率80-120%
    运输2-8 ℃
    备注For Research Use Only.

    Founded in 2019 in France, AntibodySystem Laboratories SAS is a dedicated, independent brand focused on producing high-quality protein and antibody reagents for life science research. We aim to equip researchers worldwide with reliable products for foundational studies in life sciences. AntibodySystem's comprehensive product lineup includes traditional polyclonal antibodies, housekeeping antibodies, tag antibodies, high-performance proteins, in vivo functional antibodies, low-background antibodies for flow cytometry, nanobodies with high specificity, specialized small molecule antibodies, premium phospho-specific antibodies, DNA/RNA antibodies, PEG antibodies, and more.
    Our products are designed for various research areas, supporting studies on 48 types of viruses, superbugs, parasites, cancer, Alzheimer's disease, Parkinson's disease, allergic responses, immune suppression, and immune activation.

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