胰蛋白酶(牛胰) *Sigma货号：T8802-100MG/

一般描述

胰蛋白酶分子有两个结构域：一个与酶活性位点和色氨酸残基有关；另一个与8-苯胺基萘-1-磺酸盐结合有关
应用
For trypsin digestion peptides, trypsin: peptide is used in a ratio of approximately 1:100 to 1:20. Removing adherent cells from the culture surface is a typical application of this product. The concentration of trypsin required to remove cells from their substrates mainly depends on the cell type and the age of the culture. Trypsin is also used for cell resuspension during cell culture, protein digestion in proteomics research and various gel digestion. Other applications include evaluating crystallization through membrane based techniques in a study to determine the presence of kinetic traps that limit protein folding rate and yield. Trypsin can cause adherent cells to detach from tissue culture plates for passage. Trypsin is used to study and evaluate the effect of large molecule clusters on the structural stability of human alpha lactalbumin. [1] Trypsin is also used for BN-PAGE analysis of the secretion profile of Trichoderma harzianum
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