Trizma ® 碱 *Sigma货号：T6066-100G

一般描述

Tris 碱，也称为 Trometamol 或 Trizma 碱，作为生物缓冲剂，在各种研究应用中发挥着至关重要的作用
广泛用于 TAE 和 TBE 缓冲液等缓冲液配方，其 pKa 为 8.1，非常适合将 pH 值维持在适用于大多数生物体的生理范围内(pH 7.0-9.0)
然而，研究人员在研究蛋白质时需要谨慎，因为 Tris 可能会干扰某些酶的活性
该化合物可以单独用作缓冲液或作为混合缓冲液配方的一部分，包括 Tris-EDTA (TE) 缓冲液、 Tris-乙酸盐-EDTA (TAE) 缓冲液、Tris-硼酸盐-EDTA (TBE) 缓冲液等
值得注意的是，Tris 碱是纯的、基本稳定且相对不吸湿
In laboratory environments, Tris base has been proven to be essential for preparing buffer solutions compatible with biological fluids, serving as a standard pH solution, and facilitating various experimental protocols such as lactate dehydrogenase assays, in situ hybridization, and cell protein extraction. Its multifunctionality extends to applications in cell culture, biochemistry, and molecular biology laboratories, contributing to research on cell membrane permeability and buffer preparation