卵磷脂(来源大豆) *Sigma货号：P5638-500G/

一般描述

P5638是大豆磷脂的粗提物
纯化大豆磷脂酰胆碱的典型脂肪酸组分含量约为13％ C16:0（棕榈酸），4％ C18:0（硬脂酸），10％ C18:1（油酸），64％ C18:2（亚油酸）和6％ C18:3（亚麻酸）以及其他次要脂肪酸成分
根据该组成计算得到的平均分子量约为776
纯化的磷脂酰胆碱可溶于氯仿和乙醇
其他主要的磷脂成分包括磷脂酰乙chún胺和肌醇磷脂
可能含有其他磷脂和中性脂质，如甘油三酯、生育酚和甾醇
还可能含有碳水化合物
一种已公开方法通过测量氧化指数来测定脂质体制剂中的卵磷脂酰胆碱氧化量
[1]氧化指数是指在233nm处的吸光度与215nm处的吸光度之比
选择215nm波长是因为脂肪酸羰基在该波长处几乎没有吸光度，因此可以遵循比尔定律
已有一种胆碱含量测定方法被公开
[2]

生化/生理作用

Phosphatidylcholine is the main membrane phospholipid in eukaryotic cells. In addition to being the main structural component of cell membranes, phosphatidylcholine can also serve as a reservoir for several lipid messengers. It is a source of bioactive lipids such as lysophosphatidylcholine, phosphatidic acid, diacylglycerol, platelet activating factor, and arachidonic acid. [3] Phosphatidylcholine is used to prepare vesicle suspensions, commonly referred to as liposomes or as monolayer liposomes. [4] [5] Single layer liposomes have been formed using a hexane solution containing 1% soy phosphatidylcholine. [6] The fluorescence of positively charged cyanine dye changes with membrane potential. Previous studies have used this dye to measure the membrane potential changes of red blood cells and phospholipid (egg yolk phosphatidylcholine plus cholesterol) vesicle suspensions induced by valinamycin. [7] Previous studies have incorporated purified rhodopsin into soybean phosphatidylcholine vesicles. [8] Phospholipids, the main structural phospholipids in the brain, account for approximately 15% of the total fat content; Mainly limited to gray matter