HCET细胞

HCE-T/HCE-T细胞系/HCE-T细胞株/HCE-T人角膜上皮细胞 永生化

Cell line name HCE-T

Synonyms HCET; Human Corneal Epithelial cells-Transformed; HCE; SV40-HCEC

Accession CVCL_1272

Resource Identification Initiative To cite this cell line use: HCE-T (RRID:CVCL_1272)

Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Population: Japanese.

Doubling time: 24.4 hours (PubMed=7534282).

Transformant: Ad-SV40 hybrid virus.

Omics: Deep exome analysis.

Omics: DNA methylation analysis.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Derived from site: In situ; Eye, cornea, epithelium; UBERON=UBERON_0001772.

Cell type: Epithelial cell of cornea; CL=CL_0000575.

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Sex of cell Female

Age at sampling 49Y

Category Transformed cell line

STR profile Source(s): PubMed=26900324; PubMed=29787296; RCB=RCB2280

 

Markers:

Amelogenin X

CSF1PO 9,12 (PubMed=26900324)

12 (RCB=RCB2280; PubMed=29787296)

D2S1338 19,20

D3S1358 15,17

D5S818 9,13 (PubMed=26900324; PubMed=29787296)

13 (RCB=RCB2280)

D7S820 10,13

D8S1179 11,13

D13S317 8

D16S539 9,11

D18S51 13 (PubMed=29787296)

13,15 (PubMed=26900324)

D19S433 13,14

D21S11 27,30

FGA 20

Penta D 12,15

Penta E 15,16

TH01 7,8

TPOX 8,11

vWA 18,19

 

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Publications

PubMed=7534282

Araki-Sasaki K., Ohashi Y., Sasabe T., Hayashi K., Watanabe H., Tano Y., Handa H.

An SV40-immortalized human corneal epithelial cell line and its characterization.

Invest. Ophthalmol. Vis. Sci. 36:614-621(1995)

 

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113

Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Signatures of mutation and selection in the cancer genome.

Nature 463:893-898(2010)

 

PubMed=26900324; PMCID=PMC4734150

Akin D., Newman J.R.B., McIntyre L.M., Sugrue S.P.

RNA-seq analysis of impact of PNN on gene expression and alternative splicing in corneal epithelial cells.

Mol. Vis. 22:40-60(2016)

 

PubMed=28315467; DOI=10.1016/j.ejps.2017.03.018

Juretic M., Jurisic Dukovski B., Krtalic I., Reichl S., Cetina-Cizmek B., Filipovic-Grcic J., Lovric J., Pepic I.

HCE-T cell-based permeability model: a well-maintained or a highly variable barrier phenotype?

Eur. J. Pharm. Sci. 104:23-30(2017)

 

DOI=10.22028/D291-35665

Rubelowski A.-K.

Characterization of the HCE-T cell line (RCB 2280) and its comparison with primary limbal epithelial cell culture and primary corneal epithelial cells.

Thesis MD (2018); Saarland University; Saarbrucken; Germany

 

PubMed=29787296; DOI=10.1080/02713683.2018.1480043; PMCID=PMC6590053

McDermott A.M., Baidouri H., Woodward A.M., Kam W.R., Liu Y., Chen X.-M., Ziemanski J.F., Vistisen K., Hazlett L.D., Nichols K.K., Argueso P., Sullivan D.A.

Short tandem repeat (STR) profiles of commonly used human ocular surface cell lines.

Curr. Eye Res. 43:1097-1101(2018)

 

PubMed=31927639; DOI=10.1007/s00417-019-04563-0

Rubelowski A.-K., Latta L., Katiyar P., Stachon T., Kasmann-Kellner B., Seitz B., Szentmary N.

HCE-T cell line lacks cornea-specific differentiation markers compared to primary limbal epithelial cells and differentiated corneal epithelium.

Graefes Arch. Clin. Exp. Ophthalmol. 258:565-575(2020)