JEG3细胞

JEG-3/JEG-3细胞系/JEG-3细胞株/JEG-3人绒毛膜癌细胞

Cell line name JEG-3

Synonyms Jeg-3; JEG3; Jeg3; jeg3

Accession CVCL_0363

Resource Identification Initiative To cite this cell line use: JEG-3 (RRID:CVCL_0363)

Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: COSMIC cell lines project.

Population: Caucasian.

Characteristics: Established by serial cloning into hamster cheek pouches.

Doubling time: ~36 hours (CLS=300222); ~24 hours (DSMZ=ACC-463).

Karyotypic information: Near tetraploid karyotype (PubMed=33096371).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Deep exome analysis.

Omics: Deep quantitative proteome analysis.

Omics: DNA methylation analysis.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Caution: A TP53 mutation is indicated as being at p.Gln167His (c.501G>T) in PubMed=7819056 but has not been confirmed by CCLE and Cosmic-CLP.

Derived from site: Metastatic; Fetal brain; UBERON=UBERON_0006238.

Genome ancestry Source: PubMed=30894373

 

Origin % genome

African 0.01

Native American 0.09

East Asian, North 0.97

East Asian, South 0

South Asian 0.9

European, North 71.88

European, South 26.16

Disease Gestational choriocarcinoma (NCIt: C4646)

Gestational choriocarcinoma (ORDO: Orphanet_99926)

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy Children:

CVCL_9559 (AC1 [Human gestational choriocarcinoma]) CVCL_E1LL (HyCyte JEG-3 KO-hB2M) CVCL_D6BV (HyCyte JEG-3 KO-hLUM)

CVCL_IV65 (JEG-3/A3-1A7) CVCL_6922 (JEG-3/VP16) CVCL_7167 (TEC61)

Originate from same individual CVCL_0044 ! BeWo

Sex of cell Male

Age at sampling Fetus

Category Cancer cell line

STR profile Source(s): AddexBio=C0030003/4940; ATCC=HTB-36; CCRID; CLS=300222; Cosmic-CLP=907176; DepMap=ACH-001530; DSMZ=ACC-463; KCLB=30036; PubMed=33810901

 

Markers:

Amelogenin X,Y

CSF1PO 11,12

D1S1656 14,16

D2S441 14,15

D2S1338 24

D3S1358 15 (CLS=300222)

15,16 (ATCC=HTB-36; CCRID; DepMap=ACH-001530; DSMZ=ACC-463; KCLB=30036; PubMed=33810901)

D5S818 10,11

D7S820 10,12

D8S1179 12

D10S1248 13,15

D12S391 17,24

D13S317 9,11

D16S539 13,14

D18S51 14

D19S433 13,15

D21S11 30

D22S1045 15

FGA 23,24

Penta D 9,12

Penta E 8,12

SE33 15,25.2

TH01 9,9.3

TPOX 8

vWA 16

 

Run an STR similarity search on this cell line

PubMed=62839; DOI=10.1093/jnci/56.5.911

Lieblich J.M., Weintraub B.D., Krauth G.H., Kohler P.O., Rabson A.S., Rosen S.W.

Ectopic and eutopic secretion of chorionic gonadotropin and its subunits in vitro: comparison of clonal strains from carcinomas of lung and placenta.

J. Natl. Cancer Inst. 56:911-917(1976)

 

PubMed=327080; DOI=10.1093/jnci/59.1.221

Fogh J., Fogh J.M., Orfeo T.

One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.

J. Natl. Cancer Inst. 59:221-226(1977)

 

PubMed=833871; DOI=10.1093/jnci/58.2.209

Fogh J., Wright W.C., Loveless J.D.

Absence of HeLa cell contamination in 169 cell lines derived from human tumors.

J. Natl. Cancer Inst. 58:209-214(1977)

 

PubMed=7459858

Rousset M., Zweibaum A., Fogh J.

Presence of glycogen and growth-related variations in 58 cultured human tumor cell lines of various tissue origins.

Cancer Res. 41:1165-1170(1981)

 

PubMed=6220172

Dracopoli N.C., Fogh J.

Polymorphic enzyme analysis of cultured human tumor cell lines.

J. Natl. Cancer Inst. 70:469-476(1983)

 

PubMed=2878840; DOI=10.1016/0020-7292(86)90088-3

Yamashita K., Nakamura T., Shimizu T., Katagiri M., Ikeda H.

Expression of HLA class I and class II antigens in human choriocarcinoma cell lines.

Int. J. Gynaecol. Obstet. 24:301-307(1986)

 

PubMed=3518877; DOI=10.3109/07357908609038260

Fogh J.

Human tumor lines for cancer research.

Cancer Invest. 4:157-184(1986)

 

PubMed=7937597; DOI=10.1016/0143-4004(94)90008-6

Grummer R., Hohn H.-P., Mareel M.M., Denker H.-W.

Adhesion and invasion of three human choriocarcinoma cell lines into human endometrium in a three-dimensional organ culture system.

Placenta 15:411-429(1994)

 

PubMed=7753552

Brown A.D., Lopez-Terrada D.H., Denny C.T., Lee K.A.W.

Promoters containing ATF-binding sites are de-regulated in cells that express the EWS/ATF1 oncogene.

Oncogene 10:1749-1756(1995)

 

PubMed=7819056; DOI=10.1038/bjc.1995.3; PMCID=PMC2033447

Yaginuma Y., Yamashita T., Takuma N., Katayama H., Ishikawa M.

Analysis of the p53 gene in human choriocarcinoma cell lines.

Br. J. Cancer 71:9-12(1995)

 

DOI=10.1007/0-306-46861-1_8

Ganapathy V., Prasad P.D., Leibach F.H.

Choriocarcinoma.

(In book chapter) Human cell culture. Vol. 2. Cancer cell lines part 2; Masters J.R.W., Palsson B.O. (eds.); pp.141-147; Kluwer Academic Publishers; New York; USA (1999)

 

PubMed=11994546; DOI=10.1093/molehr/8.5.485

Blanchon L., Sauvant P., Bavik C., Gallot D., Charbonne F., Alexandre-Gouabau M.-C., Lemery D., Jacquetin B., Dastugue B., Ward S., Sapin V.

Human choriocarcinoma cell line JEG-3 produces and secretes active retinoids from retinol.

Mol. Hum. Reprod. 8:485-493(2002)

 

PubMed=12966428; DOI=10.1038/sj.bjc.6601212; PMCID=PMC2376962

Li K.K.C., Goodall J., Goding C.R., Liao S.-K., Wang C.-H., Lin Y.-C., Hiraga H., Nojima T., Nagashima K., Schaefer K.-L., Lee K.A.W.

The melanocyte inducing factor MITF is stably expressed in cell lines from human clear cell sarcoma.

Br. J. Cancer 89:1072-1078(2003)

 

PubMed=16797695; DOI=10.1016/j.placenta.2006.05.001

Burleigh D.W., Kendziorski C.M., Choi Y.J., Grindle K.M., Grendell R.L., Magness R.R., Golos T.G.

Microarray analysis of BeWo and JEG3 trophoblast cell lines: identification of differentially expressed transcripts.

Placenta 28:383-389(2007)

 

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113

Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Signatures of mutation and selection in the cancer genome.

Nature 463:893-898(2010)