NCI-H2126人非小细胞肺癌细胞

NCI-H2126/NCI-H2126细胞系/NCI-H2126细胞株/NCI-H2126人非小细胞肺癌细胞

Cell line name NCI-H2126

Synonyms H2126; H-2126; NCIH2126

Accession CVCL_1532

Resource Identification Initiative To cite this cell line use: NCI-H2126 (RRID:CVCL_1532)

Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: COSMIC cell lines project.

Part of: MD Anderson Cell Lines Project.

Population: Caucasian.

Doubling time: 41.1 hours (PubMed=29681454); ~57 hours (PBCF).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Array-based CGH.

Omics: CNV analysis.

Omics: Deep exome analysis.

Omics: Deep proteome analysis.

Omics: Deep quantitative proteome analysis.

Omics: DNA methylation analysis.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.

Sequence variations

Mutation; HGNC; 11100; SMARCA4; Simple; p.Trp764Arg (c.2290T>A); Zygosity=Homozygous (Cosmic-CLP=687814; DepMap=ACH-000785).

Mutation; HGNC; 11998; TP53; Simple; p.Glu62Ter (c.184G>T); ClinVar=VCV000617472; Zygosity=Homozygous (PubMed=20557307; Cosmic-CLP=687814; DepMap=ACH-000785).

HLA typing Source: PubMed=26589293

Class I

HLA-A A*02:05,03:01

HLA-B B*08:20,15:01

HLA-C C*01:02,04:01

Class II

HLA-DQ DQA1*03:02,05:02

DQB1*06:02,06:02

HLA-DR DRB1*15:01,16:08

Genome ancestry Source: PubMed=30894373

 

Origin % genome

African 1.91

Native American 0.65

East Asian, North 3.65

East Asian, South 0

South Asian 0

European, North 58.35

European, South 35.45

Disease Lung large cell carcinoma (NCIt: C4450)

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Originate from same individual CVCL_2652 ! NCI-BL2126

Sex of cell Male

Age at sampling 65Y

Category Cancer cell line

STR profile Source(s): ATCC=CCL-256; Cosmic-CLP=687814; PubMed=11416159; PubMed=25877200

 

Markers:

Amelogenin X

CSF1PO 11

D2S1338 17,19

D3S1358 16

D5S818 11

D7S820 8,9 (ATCC=CCL-256; Cosmic-CLP=687814)

9 (PubMed=25877200)

D8S1179 12

D13S317 12,14

D16S539 12

D18S51 14

D19S433 14

D21S11 29

FGA 26

Penta D 10

Penta E 7,10

TH01 7,9.3

TPOX 8

vWA 17

 

Publications

PubMed=1311061

Mitsudomi T., Steinberg S.M., Nau M.M., Carbone D.P., D'Amico D., Bodner S.M., Oie H.K., Linnoila R.I., Mulshine J.L., Minna J.D., Gazdar A.F.

p53 gene mutations in non-small-cell lung cancer cell lines and their correlation with the presence of ras mutations and clinical features.

Oncogene 7:171-180(1992)

 

PubMed=8806092; DOI=10.1002/jcb.240630505

Phelps R.M., Johnson B.E., Ihde D.C., Gazdar A.F., Carbone D.P., McClintock P.R., Linnoila R.I., Matthews M.J., Bunn P.A. Jr., Carney D.N., Minna J.D., Mulshine J.L.

NCI-Navy Medical Oncology Branch cell line data base.

J. Cell. Biochem. Suppl. 24:32-91(1996)

 

PubMed=9559342; DOI=10.1002/(SICI)1098-2264(199804)21:4<308::AID-GCC4>3.0.CO;2-2

Virmani A.K., Fong K.M., Kodagoda D.R., McIntire D., Hung J.Y., Tonk V., Minna J.D., Gazdar A.F.

Allelotyping demonstrates common and distinct patterns of chromosomal loss in human lung cancer types.

Genes Chromosomes Cancer 21:308-319(1998)

 

PubMed=10987304

Girard L., Zochbauer-Muller S., Virmani A.K., Gazdar A.F., Minna J.D.

Genome-wide allelotyping of lung cancer identifies new regions of allelic loss, differences between small cell lung cancer and non-small cell lung cancer, and loci clustering.

Cancer Res. 60:4894-4906(2000)

 

PubMed=11030152; DOI=10.1038/sj.onc.1203815

Modi S., Kubo A., Oie H.K., Coxon A.B., Rehmatulla A., Kaye F.J.

Protein expression of the RB-related gene family and SV40 large T antigen in mesothelioma and lung cancer.

Oncogene 19:4632-4639(2000)

 

PubMed=11314036; DOI=10.1038/sj.onc.1204211

Forgacs E., Wren J.D., Kamibayashi C., Kondo M., Xu X.L., Markowitz S.D., Tomlinson G.E., Muller C.Y., Gazdar A.F., Garner H.R., Minna J.D.

Searching for microsatellite mutations in coding regions in lung, breast, ovarian and colorectal cancers.

Oncogene 20:1005-1009(2001)

 

PubMed=11416159; DOI=10.1073/pnas.121616198; PMCID=PMC35459

Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.

Short tandem repeat profiling provides an international reference standard for human cell lines.

Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

 

PubMed=14762065; DOI=10.1101/gr.2012304; PMCID=PMC327104

Bignell G.R., Huang J., Greshock J., Watt S., Butler A.P., West S., Grigorova M., Jones K.W., Wei W., Stratton M.R., Futreal P.A., Weber B., Shapero M.H., Wooster R.

High-resolution analysis of DNA copy number using oligonucleotide microarrays.

Genome Res. 14:287-295(2004)

 

PubMed=16157194; DOI=10.1016/j.cancergencyto.2005.03.007

Grigorova M., Lyman R.C., Caldas C., Edwards P.A.W.

Chromosome abnormalities in 10 lung cancer cell lines of the NCI-H series analyzed with spectral karyotyping.

Cancer Genet. Cytogenet. 162:1-9(2005)

 

PubMed=19153074; DOI=10.1093/hmg/ddp034

Medina P.P., Castillo S.D., Blanco S., Sanz-Garcia M., Largo C., Alvarez S., Yokota J., Gonzalez-Neira A., Benitez J., Clevers H.C., Cigudosa J.C., Lazo P.A., Sanchez-Cespedes M.

The SRY-HMG box gene, SOX4, is a target of gene amplification at chromosome 6p in lung cancer.

Hum. Mol. Genet. 18:1343-1352(2009)

 

PubMed=19472407; DOI=10.1002/humu.21028; PMCID=PMC2900846

Blanco R., Iwakawa R., Tang M.-Y., Kohno T., Angulo B., Pio R., Montuenga L.M., Minna J.D., Yokota J., Sanchez-Cespedes M.

A gene-alteration profile of human lung cancer cell lines.

Hum. Mutat. 30:1199-1206(2009)

 

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113

Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Signatures of mutation and selection in the cancer genome.

Nature 463:893-898(2010)