KG-1A人急性骨髓白血病细胞

KG-1a /KG-1a 细胞系/KG-1a 细胞株/KG-1a 人急性骨髓白血病细胞

Cell line name KG-1a

Synonyms KG-1A; KG1A; KG1a

Accession CVCL_1824

Resource Identification Initiative To cite this cell line use: KG-1a (RRID:CVCL_1824)

Comments Population: Caucasian.

Characteristics: Less differentiated subline of KG-1, composed of undifferentiated promyeloblasts.

Doubling time: ~45 hours (CLS=300234); ~50 hours (DSMZ=ACC-421).

Omics: Transcriptome analysis by microarray.

Derived from site: In situ; Bone marrow; UBERON=UBERON_0002371.

Sequence variations

Gene fusion; HGNC; 3688; FGFR1 + HGNC; 23098; FGFR1OP2; Name(s)=FGFR1OP2-FGFR1 (from parent cell line).

Mutation; HGNC; 7989; NRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000039648; Zygosity=Unspecified (from parent cell line).

Mutation; HGNC; 11998; TP53; Simple; c.672+1G>A; ClinVar=VCV000216078; Zygosity=Homozygous; Note=Splice donor mutation (from parent cell line).

Disease Adult acute myeloid leukemia (NCIt: C9154)

Acute myeloid leukemia (ORDO: Orphanet_519)

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy Parent: CVCL_0374 (KG-1)

Sex of cell Male

Age at sampling 59Y

Category Cancer cell line

STR profile Source(s): ATCC=CCL-246.1; CCRID=1102HUM-NIFDC00028; CLS=300234; COG; DSMZ=ACC-421; ECACC=91030101; PubMed=11416159; PubMed=25877200; RCB=RCB1928; TKG=TKG 0402

 

Markers:

Amelogenin X,Y

CSF1PO 7

D2S1338 20,23

D3S1358 15,16

D5S818 13

D7S820 8,10

D8S1179 13,14 (ATCC=CCL-246.1; CCRID=1102HUM-NIFDC00028; CLS=300234; DSMZ=ACC-421; PubMed=11416159; PubMed=25877200)

14 (COG)

D13S317 11,12

D16S539 10,11

D18S51 10.2,18

D19S433 11,16.2 (ATCC=CCL-246.1; COG)

11,17 (DSMZ=ACC-421)

16.2 (PubMed=25877200)

D21S11 28,29

FGA 22

Penta D 8,9

Penta E 7,13

TH01 7,8

TPOX 7 (PubMed=25877200)

7,9 (ATCC=CCL-246.1; CCRID=1102HUM-NIFDC00028; CLS=300234; COG; DSMZ=ACC-421; ECACC=91030101; RCB=RCB1928; TKG=TKG 0402)

vWA 14,19

PubMed=6967340; DOI=10.1182/blood.V56.2.265.265

Koeffler H.P., Billing R.J., Lusis A.J., Sparkes R.S., Golde D.W.

An undifferentiated variant derived from the human acute myelogenous leukemia cell line (KG-1).

Blood 56:265-273(1980)

 

PubMed=3094604; DOI=10.1182/blood.V68.5.1101.1101

Furley A.J.W., Reeves B.R., Mizutani S., Altass L.J., Watt S.M., Jacob M.C., van den Elsen P., Terhorst C.P., Greaves M.F.

Divergent molecular phenotypes of KG1 and KG1a myeloid cell lines.

Blood 68:1101-1107(1986)

 

PubMed=8558913

Morita S., Tsuchiya S., Fujie H., Itano M., Ohashi Y., Minegishi M., Imaizumi M., Endo M., Takano N., Konno T.

Cell surface c-kit receptors in human leukemia cell lines and pediatric leukemia: selective preservation of c-kit expression on megakaryoblastic cell lines during adaptation to in vitro culture.

Leukemia 10:102-105(1996)

 

DOI=10.1016/B978-0-12-221970-2.50457-5

Drexler H.G.

The leukemia-lymphoma cell line factsbook.

(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001)

 

PubMed=11416159; DOI=10.1073/pnas.121616198; PMCID=PMC35459

Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.

Short tandem repeat profiling provides an international reference standard for human cell lines.

Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

 

PubMed=22844360; DOI=10.3892/ol.2012.709; PMCID=PMC3402744

Pelliccia F., Ubertini V., Bosco N.

The importance of molecular cytogenetic analysis prior to using cell lines in research: the case of the KG-1a leukemia cell line.

Oncol. Lett. 4:237-240(2012)

 

PubMed=25877200; DOI=10.1038/nature14397

Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.

A resource for cell line authentication, annotation and quality control.

Nature 520:307-311(2015)