Bend.3小鼠脑微血管内皮细胞

bend.3/bend.3细胞系/bend.3细胞株/bend.3小鼠脑微血管内皮细胞株

Cell line name bEnd.3

Synonyms bEND.3; b.End3; Bend.3; bEnd3; BEND-3; BEND3; brain-derived Endothelial cells.3

Accession CVCL_0170

Resource Identification Initiative To cite this cell line use: bEnd.3 (RRID:CVCL_0170)

Comments Characteristics: Widely used for blood vascular research.

Doubling time: 26-30 hours (PubMed=14568334).

Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P03077; Murine polyomavirus (strain A2) (MPyV) middle T antigen (PyMT).

Transformant: NCBI_TaxID; 10636; Murine polyomavirus (strain A2) (MPyV).

Derived from site: In situ; Brain; UBERON=UBERON_0000955.

Cell type: Endothelial cell; CL=CL_0000115.

Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)

Breed/subspecies: BALB/c.

Hierarchy Children:

CVCL_E1JC (HyCyte bEnd.3 KO-mCdh5)

Sex of cell Sex unspecified

Age at sampling 6W

Category Transformed cell line

PubMed=2379237; DOI=10.1016/0092-8674(90)90009-4

Montesano R., Pepper M.S., Mohle-Steinlein U., Risau W., Wagner E.F., Orci L.

Increased proteolytic activity is responsible for the aberrant morphogenetic behavior of endothelial cells expressing the middle T oncogene.

Cell 62:435-445(1990)

 

PubMed=14568334; DOI=10.1016/S0006-8993(03)03443-7

Omidi Y., Campbell L., Barar J., Connell D., Akhtar S., Gumbleton M.

Evaluation of the immortalised mouse brain capillary endothelial cell line, b.End3, as an in vitro blood-brain barrier model for drug uptake and transport studies.

Brain Res. 990:95-112(2003)

 

PubMed=22224974; DOI=10.1089/scd.2011.0501

Joubin K., Richardson A., Novoa N., Tu E., Tomishima M.J.

The endothelial cell line bEnd.3 maintains human pluripotent stem cells.

Stem Cells Dev. 21:2312-2321(2012)

 

PubMed=25282873; PMCID=PMC4636729

Yuen D., Leu R.K., Tse J., Wang S., Chen L.L., Chen L.

Novel characterization of bEnd.3 cells that express lymphatic vessel endothelial hyaluronan receptor-1.

Lymphology 47:73-81(2014)