NCI-H295R人肾上腺皮质腺癌细胞

NCI-H295R/NCI-H295R细胞系/NCI-H295R细胞株/NCI-H295R人肾上腺皮质癌

Cell line name NCI-H295R

Synonyms NCI H295R; NCIH295R; H295R; H-295R; H295R-S1

Accession CVCL_0458

Resource Identification Initiative To cite this cell line use: NCI-H295R (RRID:CVCL_0458)

Comments Characteristics: Grows in a medium called Nu-Serum type I.

Doubling time: 25 hours (PubMed=29371329).

Omics: Genome sequenced.

Omics: SNP array analysis.

Omics: Transcriptome analysis by RNAseq.

Derived from site: In situ; Adrenal gland; UBERON=UBERON_0002369.

Sequence variations

Mutation; HGNC; 2514; CTNNB1; Simple; p.Ser45Pro (c.133T>C); ClinVar=VCV000017589; Zygosity=Heterozygous (from parent cell line).

Disease Adrenal cortex carcinoma (NCIt: C9325)

Adrenocortical carcinoma (ORDO: Orphanet_1501)

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy Parent: CVCL_0456 (NCI-H295)

Children:

CVCL_5V09 (HAC13) CVCL_S898 (HAC15) CVCL_5V08 (HAC50)

Sex of cell Female

Age at sampling 48Y

Category Cancer cell line

STR profile Source(s): ATCC=CRL-2128; CCRID; CLS=300483

 

Markers:

Amelogenin X

CSF1PO 10,12

D2S1338 25

D3S1358 15,16

D5S818 12

D7S820 9,12

D8S1179 13

D13S317 13

D16S539 11

D18S51 17

D19S433 13

D21S11 32.2

FGA 19.2,24

Penta D 8

Penta E 5,12

TH01 9.3

TPOX 8

vWA 17,18

 

PubMed=15

Rainey W.E., Saner K., Schimmer B.P.

Adrenocortical cell lines.

Mol. Cell. Endocrinol. 228:23-38(2004)

 

PubMed=18000308; DSamandari E., Kempna P., Nuoffer J.-M., Hofer G., Mullis P.-E., Fluck C.E.

Human adrenal corticocarcinoma NCI-H295R cells produce more androgens than NCI-H295A cells and differ in 3beta-hydroxysteroid dehydrogenase type 2 and 17,20 lyase activities.

J. Endocrinol. 19

 

PubMed=202155

Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Cancer Res. 

 

Wang T., Rainey W.E.

Human adrenocortical carcinoma cell lines.

 

 

PubMed=2

Wang T., Rowland J.G., Parmar J., Nesterova M., Seki T., Rainey W.E.

Comparison of aldosterone production among human adrenocortical cell lines.

Horm. Metab. Res. 44:245

 

PubMed=2

Nishi H., Arai H., Momiyama T.

NCI-H295R, a human adrenal cortex-derived cell line, expresses purinergic receptors linked to Ca(2)(+)-mobilization/influx and cortisol secretion.

PLoS ONE 8:E7

 

PubMed=293

Kiseljak-Vassiliades K., Zhang Y., Bagby S.M., Kar A., Pozdeyev N., Xu M., Gowan K., Sharma V., Raeburn C.D., Albuja-Cruz M.B., Jones K.L., Fishbein L., Schweppe R.E., Somerset H., Pitts T.M., Leong S., Wierman M.E.

Development of new preclinical models to advance adrenocortical carcinoma research.

Endocr. Relat. Cancer 25:4

 

PubMed=33981288; DOI

Rossini E., Tamburello M., Abate A., Beretta S., Fragni M., Cominelli M., Cosentini D., Hantel C., Bono F., Grisanti S., Poliani P.L., Tiberio G.A.M., Memo M., Sigala S., Berruti A.

Cytotoxic effect of progesterone, tamoxifen and their combination in experimental cell models of human adrenocortical cancer.

Front. Endocrinol. 12:669426.1

 

PubMed=34572026;

Wellman K., Fu R., Baldwin A., Rege J., Murphy E., Rainey W.E., Mukherjee N.

Transcriptomic response dynamics of human primary and immortalized adrenocortical cells to steroidogenic stimuli.

Cells 10:2376.1-23

 

PubMed=35563746; 

Sigala S., Bothou C., Penton D., Abate A., Peitzsch M., Cosentini D., Tiberio G.A.M., Bornstein S.R., Berruti A., Hantel C.

A comprehensive investigation of steroidogenic signaling in classical and new experimental cell models of adrenocortical carcinoma.

 

 

PubMed=35764904; DOI=

Sigala S., Rossini E., Abate A., Tamburello M., Bornstein S.R., Hantel C.

An update on adrenocortical cell lines of human origin.