MT-4人急性淋巴母细胞白血病细胞

MT-4 /MT-4 细胞系/MT-4 细胞株/MT-4 人急性淋巴母细胞白血病细胞

Cell line name MT-4

Synonyms MT4

Accession CVCL_2632

Resource Identification Initiative To cite this cell line use: MT-4 (RRID:CVCL_2632)

Comments Problematic cell line: Partially contaminated. Two different lots formerly distributed by NIH-ARP; 13 P7 3/9/92 and 150048; were shown not to be authentic MT-4 (PubMed=9261343; PubMed=31554688).

Population: Japanese.

Characteristics: Was established by co-cultivation of cells from a 50 year old male T-cell leukemia patient with cord leukocytes from a male infant (CelloPub=CLPUB00542).

Virology: HTLV-1 producing cell line. Expresses Tax. Is permissive for replication of an HIV-1 gp41 mutant lacking the cytoplasmic tail.

Doubling time: ~30 hours (Note=Lot 102799) (JCRB=JCRB1216).

Transformant: NCBI_TaxID; 11908; Human T-lymphotropic virus 1 (HTLV-1).

Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.

Cell type: T-cell; CL=CL_0000084.

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy Children:

CVCL_RW54 (MT4/HIV-1) CVCL_RW55 (MT4C5)

Sex of cell Male

Age at sampling <1D

Category Transformed cell line

STR profile Source(s): JCRB=JCRB0135; JCRB=JCRB1216; PubMed=31554688; PubMed=32938764

 

Markers:

Amelogenin X,Y

CSF1PO 11,12

D3S1358 17

D5S818 10,11

D7S820 8,9.3,10 (JCRB=JCRB1216)

8,10 (JCRB=JCRB0135; PubMed=31554688; PubMed=32938764)

D8S1179 10,15

D13S317 12

D16S539 9,12

D18S51 13

D21S11 28,30.3

FGA 23

Penta D 10,13

Penta E 5,15

TH01 7

TPOX 11

vWA 17,18

PubMed=1364232

Gyuris A., Vajda G., Foldes I.

Establishment of an MT4 cell line persistently producing infective HIV-1 particles.

Acta Microbiol. Hung. 39:271-279(1992)

 

PubMed=8927062

Susloparov M.A., Krendel'shchikov A.V., Bakhtina M.M., Bedristov A.I., Babkin I.V., Gutorov V.V., Eroshkin A.M., Mamaeva O.A.

Cloning of proviral DNA sequences of the human T-cell leukemia virus type 1 (HTLV-1) from the genome of cultured MT-4 cells.

Mol. Gen. Mikrobiol. Virusol. 1996:32-39(1996)

 

PubMed=8957066; DOI=10.1111/j.1349-7006.1996.tb03112.x; PMCID=PMC5920998

Kawasaki N., Matsuo Y., Yoshino T., Yanai H., Oka T., Teramoto N., Liu C., Kondo E., Minowada J., Akagi T.

Metastatic potential of lymphoma/leukemia cell lines in SCID mice is closely related to expression of CD44.

Jpn. J. Cancer Res. 87:1070-1077(1996)

 

PubMed=9261343; DOI=10.1128/JVI.71.9.6277-6278.1997; PMCID=PMC191899

Jeang K.-T., Derse D.D., Matocha M., Sharma O.

Expression status of Tax protein in human T-cell leukemia virus type 1-transformed MT4 cells: recall of MT4 cells distributed by the NIH AIDS Research and Reference Reagent Program.

J. Virol. 71:6277-6278(1997)

 

PubMed=29892436; DOI=10.1098/rsos.172472; PMCID=PMC5990783

Shioda S., Kasai F., Watanabe K., Kawakami K., Ohtani A., Iemura M., Ozawa M., Arakawa A., Hirayama N., Kawaguchi E., Tano T., Miyata S., Satoh M., Shimizu N., Kohara A.

Screening for 15 pathogenic viruses in human cell lines registered at the JCRB Cell Bank: characterization of in vitro human cells by viral infection.

R. Soc. Open Sci. 5:172472-172472(2018)

 

PubMed=31554688; DOI=10.1128/JVI.01390-19; PMCID=PMC6880167

Fernandez M.V., Delviks-Frankenberry K.A., Scheiblin D.A., Happel C., Pathak V.K., Freed E.O.

Authentication analysis of MT-4 cells distributed by the National Institutes of Health AIDS Reagent Program.

J. Virol. 93:e01390.19.1-e01390.19.8(2019)

 

PubMed=32938764; DOI=10.1128/JVI.01334-20; PMCID=PMC7654282

Fernandez M.V., Hoffman H.K., Pezeshkian N., Tedbury P.R., van Engelenburg S.B., Freed E.O.

Elucidating the basis for permissivity of the MT-4 T-cell line to replication of an HIV-1 mutant lacking the gp41 cytoplasmic tail.

J. Virol. 94:e01334.20.1-e01334.20.27(2020)