HeLa人宫颈癌细胞

Hela /Hela 细胞系/Hela 细胞株/Hela 人宫颈腺癌细胞

Cell line name HeLa

Synonyms HELA; Hela; He La; He-La; HeLa-CCL2; Henrietta Lacks cells; Helacyton gartleri

Accession CVCL_0030

Resource Identification Initiative To cite this cell line use: HeLa (RRID:CVCL_0030)

Comments Group: Space-flown cell line (cellonaut).

Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: COSMIC cell lines project.

Part of: MD Anderson Cell Lines Project.

Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).

Population: African American.

Virology: HeLa has 5 five HPV18 integration sites: three on normal chromosomes 8 at 8q24 and two on derivative chromosomes, der(5)t(5;22;8)(q11;q11q13;q24) and der(22)t(8;22)(q24;q13).

Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).

Doubling time: 1.3 days (PubMed=29156801); ~48 hours (DSMZ=ACC-57).

Microsatellite instability: Stable (MSS) (PubMed=12661003; Sanger).

Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).

Omics: Cell surface proteome.

Omics: CNV analysis.

Omics: Deep antibody staining analysis.

Omics: Deep exome analysis.

Omics: Deep membrane proteome analysis.

Omics: Deep phosphoproteome analysis.

Omics: Deep proteome analysis.

Omics: Deep quantitative proteome analysis.

Omics: DNA methylation analysis.

Omics: Genome sequenced.

Omics: Glycoproteome analysis by proteomics.

Omics: Myristoylated proteins analysis by proteomics.

Omics: Protein expression by reverse-phase protein arrays.

Omics: Secretome proteome analysis by 2D-DE/MS.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Omics: Transcriptome analysis by single cell RNAseq.

Omics: Virome analysis using proteomics.

Anecdotal: The HeLa cell line which was established in February 1951 is the oldest human immortal cell line.

Anecdotal: The fascinating story of the HeLa cell line and of Henrietta Lacks from whom these cells originate are described in the book of Rebecca Skloot (CelloPub=CLPUB00377).

Anecdotal: The HeLa cell line and its story inspired Australian artist Cynthia Verspaget to embark in 2003 on a artistic project 'The Anarchy Cell Line' (TAnCL) where she mixed her blood with HeLa cells. This work later spawned a PhD thesis (CelloPub=CLPUB00376) where among other things she makes the observation that two main taxonomical distinctions present in the zombie, living/dead and human/nonhuman, are also present in the HeLa cell line.

Anecdotal: Was flown since the 1960s on at least ten different space missions: Korabl-Sputnik-2, Vostok-1, Vostok-4, Vostok-5 and Vostok-6, Voshkod 1 and Zond-5, Discoverer XVIII, Progress M-35/Mir and Shuttle STS-89.

Miscellaneous: HeLa is the most frequent contributor to cell lines contamination.

Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.

PubMed=15302935; DOI=10.1073/pnas.0404720101; PMCID=PMC514446

Beausoleil S.A., Jedrychowski M.P., Schwartz D., Elias J.E., Villen J., Li J.-X., Cohn M.A., Cantley L.C., Gygi S.P.

Large-scale characterization of HeLa cell nuclear phosphoproteins.

Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004)

 

PubMed=15531914; DOI=10.1038/sj.onc.1208235

Baldus S.E., Schwarz E., Lohrey C., Zapatka M., Landsberg S., Hahn S.A., Schmidt D., Dienes H.-P., Schmiegel W.H., Schwarte-Waldhoff I.

Smad4 deficiency in cervical carcinoma cells.

Oncogene 24:810-819(2005)

 

PubMed=15901131; DOI=10.1016/j.prp.2005.01.002

Murai Y., Hayashi S., Takahashi H., Tsuneyama K., Takano Y.

Correlation between DNA alterations and p53 and p16 protein expression in cancer cell lines.

Pathol. Res. Pract. 201:109-115(2005)

 

PubMed=17311676; DOI=10.1186/1471-2164-8-53; PMCID=PMC1805756

Kloth J.N., Oosting J., van Wezel T., Szuhai K., Knijnenburg J., Gorter A., Kenter G.G., Fleuren G.J., Jordanova E.S.

Combined array-comparative genomic hybridization and single-nucleotide polymorphism-loss of heterozygosity analysis reveals complex genetic alterations in cervical cancer.

BMC Genomics 8:53.1-53.13(2007)

 

PubMed=19450234; DOI=10.2144/000113089; PMCID=PMC2696096

Rahbari R., Sheahan T., Modes V., Collier P., Macfarlane C.M., Badge R.M.

A novel L1 retrotransposon marker for HeLa cell line identification.

BioTechniques 46:277-284(2009)

 

PubMed=19722756; DOI=10.5858/133.9.1463

Lucey B.P., Nelson-Rees W.A., Hutchins G.M.

Henrietta Lacks, HeLa cells, and cell culture contamination.

Arch. Pathol. Lab. Med. 133:1463-1467(2009)

 

CLPUB00377

Skloot R.L.

The immortal life of Henrietta Lacks.

(In book) ISBN 9781400052172; pp.1-400; Random House; New York; USA (2010)

 

CLPUB00468

Javitt G.H.

Why not take all of me? Reflections on the immortal life of Henrietta Lacks and the status of participants in research using human specimens.

Minn. J. Law Sci. Technol. 11:713-755(2010)

 

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022

Karger A., Bettin B., Lenk M., Mettenleiter T.C.

Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.

J. Virol. Methods 164:116-121(2010)

 

PubMed=21269460; DOI=10.1186/1752-0509-5-17; PMCID=PMC3039570

Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Burckstummer T., Bennett K.L., Superti-Furga G., Colinge J.

Initial characterization of the human central proteome.

BMC Syst. Biol. 5:17.1-17.13(2011)

 

PubMed=22068331; DOI=10.1038/msb.2011.81; PMCID=PMC3261714

Nagaraj N., Wisniewski J.R., Geiger T., Cox J., Kircher M., Kelso J., Paabo S., Mann M.

Deep proteome and transcriptome mapping of a human cancer cell line.

Mol. Syst. Biol. 7:548-548(2011)

 

PubMed=21937730; DOI=10.1074/mcp.M111.011429; PMCID=PMC3316722

Boisvert F.-M., Ahmad Y., Gierlinski M., Charriere F., Lamont D., Scott M., Barton G., Lamond A.I.

A quantitative spatial proteomics analysis of proteome turnover in human cells.

Mol. Cell. Proteomics 11:M111.011429-M111.011429(2012)

 

PubMed=22278370; DOI=10.1074/mcp.M111.014050; PMCID=PMC3316730

Geiger T., Wehner A., Schaab C., Cox J., Mann M.

Comparative proteomic analysis of eleven common cell lines reveals ubiquitous but varying expression of most proteins.

Mol. Cell. Proteomics 11:M111.014050-M111.014050(2012)

 

PubMed=22412903; DOI=10.1371/journal.pone.0032667; PMCID=PMC3296745

Vazquez-Mena O., Medina-Martinez I., Juarez-Torres E., Barron V., Espinosa A., Villegas-Sepulveda N., Gomez-Laguna L., Nieto-Martinez K., Orozco L., Roman-Bassaure E., Munoz Cortez S., Borges Ibanez M., Venegas-Vega C.A., Guardado-Estrada M., Rangel-Lopez A., Kofman S., Berumen J.

Amplified genes may be overexpressed, unchanged, or downregulated in cervical cancer cell lines.

PLoS ONE 7:E32667-E32667(2012)

 

CLPUB00507

Pultarova T.

HeLa cells: immortal space travellers.

Space Saf. Mag. 7:10-12(2013)

 

PubMed=23205564; DOI=10.1021/pr300859k

Malerod H., Graham R.L.J., Sweredoski M.J., Hess S.

Comprehensive profiling of N-linked glycosylation sites in HeLa cells using hydrazide enrichment.

J. Proteome Res. 12:248-259(2013)