HCT-15人结肠癌细胞

HCT-15/HCT-15细胞系/HCT-15细胞株/HCT-15人结肠癌细胞

Cell line name HCT 15

Synonyms HCT-15; HCT.15; HCT15

Accession CVCL_0292

Resource Identification Initiative To cite this cell line use: HCT 15 (RRID:CVCL_0292)

Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: COSMIC cell lines project.

Part of: JFCR39 cancer cell line panel.

Part of: KuDOS 95 cell line panel.

Part of: MD Anderson Cell Lines Project.

Part of: NCI RAS program mutant KRAS cell line panel.

Part of: NCI-60 cancer cell line panel.

Part of: PI3K genetic alteration cell panel (ATCC TCP-1028).

Part of: RAS genetic alteration cell panel (ATCC TCP-1031).

Population: Caucasian.

Doubling time: 19 hours (PubMed=427742); ~20 hours (PubMed=7139607); 29.57 hours (PubMed=25944804); 15 hours (CLS=300229); ~20-25 hours (DSMZ=ACC-357); 20.6 hours (NCI-DTP=HCT-15).

Microsatellite instability: Instable (MSI-high) (PubMed=11526487; PubMed=24042735; PubMed=24755471; PubMed=25926053; PubMed=28683746; PubMed=31068700; Sanger).

Omics: Array-based CGH.

Omics: CNV analysis.

Omics: CRISPR phenotypic screen.

Omics: Deep exome analysis.

Omics: Deep proteome analysis.

Omics: Deep quantitative phosphoproteome analysis.

Omics: Deep quantitative proteome analysis.

Omics: DNA methylation analysis.

Omics: Fluorescence phenotype profiling.

Omics: lncRNA expression profiling.

Omics: Metabolome analysis.

Omics: miRNA expression profiling.

Omics: N-glycan profiling.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Misspelling: HTC-15; Note=Occasionally.

Misspelling: HTC15; Note=Occasionally.

Derived from site: In situ; Colon; UBERON=UBERON_0001155.

Cell type: Epithelial cell of colon; CL=CL_0011108.

PubMed=7621404; DOI=10.1016/0165-4608(94)00225-z

Chen T.-R., Dorotinsky C.S., McGuire L.J., Macy M.L., Hay R.J.

DLD-1 and HCT-15 cell lines derived separately from colorectal carcinomas have totally different chromosome changes but the same genetic origin.

Cancer Genet. Cytogenet. 81:103-108(1995)

 

PubMed=9000147

Cottu P.-H., Muzeau F., Estreicher A., Flejou J.-F., Iggo R.D., Thomas G., Hamelin R.

Inverse correlation between RER+ status and p53 mutation in colorectal cancer cell lines.

Oncogene 13:2727-2730(1996)

 

PubMed=9000572

Hoang J.-M., Cottu P.-H., Thuille B., Salmon R.J., Thomas G., Hamelin R.

BAT-26, an indicator of the replication error phenotype in colorectal cancers and cell lines.

Cancer Res. 57:300-303(1997)

 

PubMed=9809040; DOI=10.1016/S0165-4608(98)00081-8

Vermeulen S.J., Chen T.-R., Speleman F., Nollet F., Van Roy F.M., Mareel M.M.

Did the four human cancer cell lines DLD-1, HCT-15, HCT-8, and HRT-18 originate from one and the same patient?

Cancer Genet. Cytogenet. 107:76-79(1998)

 

PubMed=10700174; DOI=10.1038/73432

Ross D.T., Scherf U., Eisen M.B., Perou C.M., Rees C., Spellman P.T., Iyer V.R., Jeffrey S.S., van de Rijn M., Waltham M.C., Pergamenschikov A., Lee J.C.F., Lashkari D., Shalon D., Myers T.G., Weinstein J.N., Botstein D., Brown P.O.

Systematic variation in gene expression patterns in human cancer cell lines.

Nat. Genet. 24:227-235(2000)

 

PubMed=10737795; DOI=10.1073/pnas.97.7.3352; PMCID=PMC16243

Rowan A.J., Lamlum H., Ilyas M., Wheeler J., Straub J., Papadopoulou A., Bicknell D.C., Bodmer W.F., Tomlinson I.P.M.

APC mutations in sporadic colorectal tumors: a mutational 'hotspot' and interdependence of the 'two hits'.

Proc. Natl. Acad. Sci. U.S.A. 97:3352-3357(2000)

 

PubMed=11314036; DOI=10.1038/sj.onc.1204211

Forgacs E., Wren J.D., Kamibayashi C., Kondo M., Xu X.L., Markowitz S.D., Tomlinson G.E., Muller C.Y., Gazdar A.F., Garner H.R., Minna J.D.

Searching for microsatellite mutations in coding regions in lung, breast, ovarian and colorectal cancers.

Oncogene 20:1005-1009(2001)

 

PubMed=11414198; DOI=10.1007/s004320000207

Lahm H., Andre S., Hoeflich A., Fischer J.R., Sordat B., Kaltner H., Wolf E., Gabius H.-J.

Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures.

J. Cancer Res. Clin. Oncol. 127:375-386(2001)

 

PubMed=11416159; DOI=10.1073/pnas.121616198; PMCID=PMC35459

Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.

Short tandem repeat profiling provides an international reference standard for human cell lines.

Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

 

PubMed=11526487; DOI=10.1038/sj.onc.1204611

Gayet J., Zhou X.-P., Duval A., Rolland S., Hoang J.-M., Cottu P.-H., Hamelin R.

Extensive characterization of genetic alterations in a series of human colorectal cancer cell lines.

Oncogene 20:5025-5032(2001)

 

PubMed=11668190; DOI=10.1177/002215540104901105

Quentmeier H., Osborn M., Reinhardt J., Zaborski M., Drexler H.G.

Immunocytochemical analysis of cell lines derived from solid tumors.

J. Histochem. Cytochem. 49:1369-1378(2001)

 

PubMed=12584437; DOI=10.1159/000068544

Melcher R., Koehler S., Steinlein C., Schmid M., Mueller C.R., Luehrs H., Menzel T., Scheppach W., Moerk H., Scheurlen M., Koehrle J., Al-Taie O.

Spectral karyotype analysis of colon cancer cell lines of the tumor suppressor and mutator pathway.

Cytogenet. Genome Res. 98:22-28(2002)