SiHa人子宫颈鳞癌细胞

SiHa/ SiHa细胞系/ SiHa细胞株/ SiHa人子宫颈鳞癌细胞

Cell line name SiHa

Synonyms Siha; SIHA

Accession CVCL_0032

Resource Identification Initiative To cite this cell line use: SiHa (RRID:CVCL_0032)

Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: COSMIC cell lines project.

Population: Japanese.

Virology: Contains a complete HPV16 genome with a disruption of the E2 region and a small deletion (PubMed=24201445).

Doubling time: 2.6 days (PubMed=29156801); 17 hours (Note=In 10% FBS), 21 hours (Note=In 5% FBS) (PubMed=30760827).

Microsatellite instability: Stable (MSS) (Sanger).

Transformant: NCBI_TaxID; 333760; Human papillomavirus type 16 (HPV16).

Omics: CNV analysis.

Omics: Deep antibody staining analysis.

Omics: Deep exome analysis.

Omics: Deep quantitative proteome analysis.

Omics: DNA methylation analysis.

Omics: Protein expression by reverse-phase protein arrays.

Omics: Secretome proteome analysis by 2D-DE/MS.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.

PubMed=833871; DOI=10.1093/jnci/58.2.209

Fogh J., Wright W.C., Loveless J.D.

Absence of HeLa cell contamination in 169 cell lines derived from human tumors.

J. Natl. Cancer Inst. 58:209-214(1977)

 

PubMed=2990217; PMCID=PMC1888002

Yee C., Krishnan-Hewlett I., Baker C.C., Schlegel R., Howley P.M.

Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines.

Am. J. Pathol. 119:361-366(1985)

 

PubMed=3518877; DOI=10.3109/07357908609038260

Fogh J.

Human tumor lines for cancer research.

Cancer Invest. 4:157-184(1986)

 

PubMed=3029430; DOI=10.1128/JVI.61.4.962-971.1987; PMCID=PMC254051

Baker C.C., Phelps W.C., Lindgren V., Braun M.J., Gonda M.A., Howley P.M.

Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines.

J. Virol. 61:962-971(1987)

 

PubMed=3029760; DOI=10.1073/pnas.84.4.1070; PMCID=PMC304363

Durst M., Croce C.M., Gissmann L., Schwarz E., Huebner K.

Papillomavirus sequences integrate near cellular oncogenes in some cervical carcinomas.

Proc. Natl. Acad. Sci. U.S.A. 84:1070-1074(1987)

 

PubMed=1394225

Somers K.D., Merrick M.A., Lopez M.E., Incognito L.S., Schechter G.L., Casey G.

Frequent p53 mutations in head and neck cancer.

Cancer Res. 52:5997-6000(1992)

 

PubMed=10423141; DOI=10.1099/0022-1317-80-7-1725

Meissner J.D.

Nucleotide sequences and further characterization of human papillomavirus DNA present in the CaSki, SiHa and HeLa cervical carcinoma cell lines.

J. Gen. Virol. 80:1725-1733(1999)

 

PubMed=15531914; DOI=10.1038/sj.onc.1208235

Baldus S.E., Schwarz E., Lohrey C., Zapatka M., Landsberg S., Hahn S.A., Schmidt D., Dienes H.-P., Schmiegel W.H., Schwarte-Waldhoff I.

Smad4 deficiency in cervical carcinoma cells.

Oncogene 24:810-819(2005)

 

PubMed=17311676; DOI=10.1186/1471-2164-8-53; PMCID=PMC1805756

Kloth J.N., Oosting J., van Wezel T., Szuhai K., Knijnenburg J., Gorter A., Kenter G.G., Fleuren G.J., Jordanova E.S.

Combined array-comparative genomic hybridization and single-nucleotide polymorphism-loss of heterozygosity analysis reveals complex genetic alterations in cervical cancer.

BMC Genomics 8:53.1-53.13(2007)

 

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113

Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Signatures of mutation and selection in the cancer genome.

Nature 463:893-898(2010)