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T25
DH82/DH82细胞系/DH82细胞株/DH82狗肾恶性组织细胞增生症细胞
Cell line name DH82
Synonyms DH-82; DH 82
Accession CVCL_2018
Resource Identification Initiative To cite this cell line use: DH82 (RRID:CVCL_2018)
Comments Part of: FACC canine tumor cell line panel.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-10389.
Characteristics: Can be infected by Trypanosoma cruzi which can then replicate and differentiate inside the cell.
Characteristics: Susceptible to infection by Ehrlichia ruminantium (PubMed=11585095; PubMed=12234001).
Virology: Supports the replication of equine infectious anemia virus (EIAV) (PubMed=11377712).
Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
Doubling time: 26 hours (PubMed=27257868).
Omics: Deep exome analysis.
Omics: Transcriptome analysis by microarray.
Sequence variations
Mutation; VGNC; 45168; PTPN11; Simple; p.Gly503Val (c.1508G>T); Zygosity=Homozygous (PubMed=31175136).
Disease Canine histiocytic sarcoma (NCIt: C128125)
Species of origin Canis lupus familiaris (Dog) (Canis familiaris) (NCBI Taxonomy: 9615)
Breed/subspecies: Golden Retriever; VBO=VBO_0200610.
Hierarchy Children:
CVCL_3461 (DH82ECOK)
Sex of cell Male
Age at sampling 10Y
Category Cancer cell line
PubMed=11585095
Zweygarth E., Josemans A.I.
Continuous in vitro propagation of Cowdria ruminantium (Welgevonden stock) in a canine macrophage-monocyte cell line.
Onderstepoort J. Vet. Res. 68:155-157(2001)
PubMed=12234001
Zweygarth E., Josemans A.I., Van Strijp M.F., Van Heerden H., Allsopp M.T.E.P., Allsopp B.A.
The Kumm isolate of Ehrlichia ruminantium: in vitro isolation, propagation and characterization.
Onderstepoort J. Vet. Res. 69:147-153(2002)
PubMed=21908323; DOI=10.1177/1040638711408064
O'Donoghue L.E., Rivest J.P., Duval D.L.
Polymerase chain reaction-based species verification and microsatellite analysis for canine cell line validation.
J. Vet. Diagn. Invest. 23:780-785(2011)
PubMed=25534080; DOI=10.1016/j.vetimm.2014.11.006
Heinrich F., Contioso V.B., Stein V.M., Carlson R., Tipold A., Ulrich R., Puff C., Baumgartner W., Spitzbarth I.
Passage-dependent morphological and phenotypical changes of a canine histiocytic sarcoma cell line (DH82 cells).
Vet. Immunol. Immunopathol. 163:86-92(2015)
PubMed=27257868; DOI=10.1371/journal.pone.0156689; PMCID=PMC4892608
Maeda J., Froning C.E., Brents C.A., Rose B.J., Thamm D.H., Kato T.A.
Intrinsic radiosensitivity and cellular characterization of 27 canine cancer cell lines.
PLoS ONE 11:E0156689-E0156689(2016)
PubMed=26842912; DOI=10.1111/vco.12197
Hartley G., Faulhaber E.A., Caldwell A., Coy J., Kurihara J.N., Guth A., Regan D.P., Dow S.W.
Immune regulation of canine tumour and macrophage PD-L1 expression.
Vet. Comp. Oncol. 15:534-549(2017)
PubMed=27197945; DOI=10.1111/vco.12192; PMCID=PMC5656225
Fowles J.S., Dailey D.D., Gustafson D.L., Thamm D.H., Duval D.L.
The Flint Animal Cancer Center (FACC) canine tumour cell line panel: a resource for veterinary drug discovery, comparative oncology and translational medicine.
Vet. Comp. Oncol. 15:481-492(2017)
PubMed=28620374; DOI=10.3389/fimmu.2017.00604; PMCID=PMC5449653
Mendonca P.H.B., da Rocha R.F.D.B., de Braz Moraes J.B., LaRocque-de-Freitas I.F., Logullo J., Morrot A., Nunes M.P., Freire-de-Lima C.G., Decote-Ricardo D.
Canine macrophage DH82 cell line as a model to study susceptibility to Trypanosoma cruzi infection.
Front. Immunol. 8:604.1-604.11(2017)
PubMed=31175136; DOI=10.1158/1535-7163.MCT-18-1346; PMCID=PMC6679748
Das S., Idate R., Cronise K.E., Gustafson D.L., Duval D.L.
Identifying candidate druggable targets in canine cancer cell lines using whole-exome sequencing.
Mol. Cancer Ther. 18:1460-1471(2019)
PubMed=33389257; DOI=10.1007/s10096-020-04106-0; PMCID=PMC7778494
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)
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文献和实验*发表【中文论文】请标注：由上海酶研生物科技有限公司提供；
*发表【英文论文】请标注：From Shanghai EK-Bioscience Biotechnology Co., Ltd.
佚名 恶性组织细胞增生症（malignant histiocytosis）是组织细胞及其前身细胞进行性恶性增生引起的一种全身性疾病。可发生于任何年龄，较多见于儿童和青壮年。男性比女性多见，约为2～3：1。临床表现为不规则发热，乏力，消瘦，全身淋巴结肿大，肝、脾肿大，有时可有皮肤损害。晚期可出现黄疸、 贫血 、白细胞和血
网络 第七节 恶性组织细胞增生症 恶性组织细胞增生症（malignant histiocytosis）是组织细胞及其前身细胞进行性恶性增生引起的一种全身性疾病。可发生于任何年龄，较多见于儿童和青壮年。男性比女性多见，约为2～3：1。临床表现为不规则发热，乏力，消瘦，全身淋巴结肿大，肝、脾肿大，有时可有皮肤损害。晚期可出现黄疸、
「铲屎官」注意了！剑桥大学揭示这些基因的改变或让猫猫狗狗成为
iBMDM 和犬的巨噬样细胞 DH82。结果表明小鼠巨噬细胞感染 2 小时后开始裂解，而犬的巨噬样细胞经过 12 小时仍存活，表明犬的细胞对于鼠伤寒沙门菌的耐受性更高。接着，研究者在小鼠模型上通过 CRISPR-Cas9 技术构建了与 caspase-1/-4 融合蛋白催化作用相当的重组蛋白。小鼠细胞在脂多糖（LPS）刺激下很快裂解，而犬的巨噬样细胞对于 LPS 的刺激则无反应。进一步的体外实验表明，caspase-1/-4 融合蛋白能激活 IL-1β 并且同时切割消皮素 D。图片来源：Cell
