RGC-5小鼠视网膜神经节细胞

RGC-5/RGC-5细胞系/RGC-5细胞株/RGC-5小鼠视网膜神经节细胞株

Cell line name RGC-5

Synonyms RGC5

Accession CVCL_4059

Resource Identification Initiative To cite this cell line use: RGC-5 (RRID:CVCL_4059)

Comments Group: Patented cell line.

Problematic cell line: Misidentified. Originally thought to be of rat origin but found to be from mouse (PubMed=19443730; PubMed=24664692). Is probably identical to 661W (PubMed=23975727; PubMed=24021353). But the originator of the 661W cell is partially contesting this claim (PubMed=24472671).

Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00472.

Registration: International Depositary Authority, American Type Culture Collection (ATCC); PTA-6600.

Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P03070; SV40 large T antigen (Note=Under the control of the IRBP promoter).

Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).

Derived from site: In situ; Eye, retina; UBERON=UBERON_0000966.

Cell type: Retinal cone cell; CL=CL_0000573.

Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)

Breed/subspecies: C57BL/6N transgenic.

Hierarchy Parent: CVCL_6240 (661W)

Sex of cell Male

Category Transformed cell line

PubMed=11165366; DOI=10.1016/S0169-328X(00)00224-2

Krishnamoorthy R.R., Agarwal P., Prasanna G., Vopat K., Lambert W.S., Sheedlo H.J., Pang I.-H., Shade D.L., Wordinger R.J., Yorio T., Clark A.F., Agarwal N.

Characterization of a transformed rat retinal ganglion cell line.

Brain Res. Mol. Brain Res. 86:1-12(2001)

 

Patent=US7303915

Levin L.A.

In vitro-differentiated retinal ganglion cells and method for producing same.

Patent number US7303915, 04-Dec-2007

 

PubMed=19443730; DOI=10.1167/iovs.09-3484

Van Bergen N.J., Wood J.P.M., Chidlow G., Trounce I.A., Casson R.J., Ju W.-K., Weinreb R.N., Crowston J.G.

Recharacterization of the RGC-5 retinal ganglion cell line.

Invest. Ophthalmol. Vis. Sci. 50:4267-4272(2009)

 

PubMed=23975727; DOI=10.1167/iovs.13-12085

Krishnamoorthy R.R., Clark A.F., Daudt D., Vishwanatha J.K., Yorio T.

A forensic path to RGC-5 cell line identification: lessons learned.

Invest. Ophthalmol. Vis. Sci. 54:5712-5719(2013)

 

PubMed=24021353; DOI=10.1016/j.exer.2013.08.002

Clark A.F., Tamm E.R., Al-Ubaidi M.R., Hollyfield J.G.

On the use of immortalized ocular cell lines in vision research: the unfortunate story of RGC-5.

Exp. Eye Res. 116:433-433(2013)

 

PubMed=24472671; DOI=10.1016/j.exer.2013.10.012; PMCID=PMC3906592

Al-Ubaidi M.R.

RGC-5: are they really 661W? The saga continues.

Exp. Eye Res. 119:115-115(2014)

 

PubMed=24664692; DOI=10.1007/978-1-4614-3209-8_19

Sippl C., Tamm E.R.

What is the nature of the RGC-5 cell line?

Adv. Exp. Med. Biol. 801:145-154(2014)

 

PubMed=29203899; DOI=10.1038/s41598-017-17241-0; PMCID=PMC5715133

Sayyad Z., Sirohi K., Radha V., Swarup G.

661W is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively.

Sci. Rep. 7:16855-16855(2017)