p815

P815/P815细胞系/P815细胞株/P815小鼠肥大细胞瘤细胞

Cell line name P815

Synonyms P-815; P 815

Accession CVCL_2154

Resource Identification Initiative To cite this cell line use: P815 (RRID:CVCL_2154)

Comments Part of: Tumor Immunology Bank (TIB) collection from Salk (transferred to ATCC in 1981).

Doubling time: ~18-22 hours (DSMZ=ACC-1).

Transformant: ChEBI; CHEBI:34342; 3-methylcholanthrene (3-MC; 20-methylcholanthrene; 20-MC; MCA).

Omics: SNP array analysis.

Misspelling: P825; Note=Occasionally.

Sequence variations

Mutation; MGI; MGI:96677; Kit; Simple; p.Asp818Tyr (c.2452G>T) (D814Y); Zygosity=Unspecified (PubMed=7513208; PubMed=10210327).

Disease Mouse mast cell neoplasm (NCIt: C21632)

Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)

Breed/subspecies: DBA/2.

Hierarchy Children:

CVCL_A9JC (P815 Hsp65-IL-2) CVCL_4Z63 (P815 MB) CVCL_4269 (P815-1-1)

CVCL_F735 (P815-X2) CVCL_C0DF (P815.Trh4)

Sex of cell Male

Category Cancer cell line

STR profile Source(s): DSMZ=ACC-1; PubMed=31220119

 

Markers:

Mouse STR 1-1 16,17

Mouse STR 1-2 14,17,18

Mouse STR 2-1 15

Mouse STR 3-2 13,14

Mouse STR 4-2 20.3

Mouse STR 5-5 13 (PubMed=31220119)

13,14 (DSMZ=ACC-1)

Mouse STR 6-4 17 (DSMZ=ACC-1)

17,18 (PubMed=31220119)

Mouse STR 6-7 12 (PubMed=31220119)

12,13 (DSMZ=ACC-1)

Mouse STR 7-1 27

Mouse STR 8-1 16

Mouse STR 11-2 16,17,18

Mouse STR 12-1 16,17 (DSMZ=ACC-1)

17 (PubMed=31220119)

Mouse STR 13-1 17,18

Mouse STR 15-3 20.3,21.3 (PubMed=31220119)

21.3 (DSMZ=ACC-1)

Mouse STR 17-2 17

Mouse STR 18-3 17 (PubMed=31220119)

17,18 (DSMZ=ACC-1)

Mouse STR 19-2 12

Mouse STR X-1 27 (PubMed=31220119)

27,28 (DSMZ=ACC-1)

 

Run an STR similarity search on this cell line

Web pages

Publications

PubMed=4208429; DOI=10.1038/249049a0

Ralph P., Nakoinz I.

Lipopolysaccharides inhibit lymphosarcoma cells of bone marrow orgin.

Nature 249:49-51(1974)

 

PubMed=1234049; DOI=10.1002/eji.1830050208

Kiessling R., Klein E., Wigzell H.

'Natural' killer cells in the mouse. I. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Specificity and distribution according to genotype.

Eur. J. Immunol. 5:112-117(1975)

 

DOI=10.2220/biomedres.5.19

Yanagisawa M., Hasegawa H., Ichiyama A., Hosoda S., Nakamura W.

Comparison of serotonin-producing murine mastocytomas, P-815 and FMA3: determination of tryptophan hydroxylase, aromatic L-amino acid decarboxylase, and cellular concentration of tryptophan, 5- hydroxytryprophan, 5-hydroxytryptamine and 5-hydroxyindoleacetic acid.

Biomed. Res. 5:19-28(1984)

 

PubMed=3792703; DOI=10.1111/j.1432-0436.1986.tb00568.x

Leenen P.J.M., Jansen A.M.A.C., van Ewijk W.

Murine macrophage cell lines can be ordered in a linear differentiation sequence.

Differentiation 32:157-164(1986)

 

PubMed=7513208; DOI=10.1182/blood.V83.9.2619.2619

Tsujimura T., Furitsu T., Morimoto M., Isozaki K., Nomura S., Matsuzawa Y., Kitamura Y., Kanakura Y.

Ligand-independent activation of c-kit receptor tyrosine kinase in a murine mastocytoma cell line P-815 generated by a point mutation.

Blood 83:2619-2626(1994)

 

PubMed=10210327; DOI=10.1016/S0301-472X(98)00075-7

London C.A., Galli S.J., Yuuki T., Hu Z.-Q., Helfand S.C., Geissler E.N.

Spontaneous canine mast cell tumors express tandem duplications in the proto-oncogene c-kit.

Exp. Hematol. 27:689-697(1999)

 

PubMed=18432777; DOI=10.1002/0471142735.im2004s43

Gajewski T.F., Markiewicz M.A., Uyttenhove C.

The P815 mastocytoma tumor model.

Curr. Protoc. Immunol. 43:20.4.1-20.4.18(2001)

 

PubMed=25277546; DOI=10.1186/1471-2164-15-847; PMCID=PMC4198738

Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. 3rd, Pardo-Manuel de Villena F.

SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.

BMC Genomics 15:847.1-847.11(2014)

 

PubMed=31220119; DOI=10.1371/journal.pone.0218412; PMCID=PMC6586308

Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J.-L., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B.A., Gollin S.M., Lewis D.W., Reid Y.A.

Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.

PLoS ONE 14:E0218412-E0218412(2019)