MAC-T

MAC-T细胞MAC-T细胞MAC-T牛乳腺上皮细胞

Cell line name MAC-T

Synonyms Mac-T; MacT; MAC-T3; Mammary Alveolar Cells-large T antigen

Accession CVCL_U226

Resource Identification Initiative To cite this cell line use: MAC-T (RRID:CVCL_U226)

Comments Group: Patented cell line.

Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-10274.

Doubling time: ~17 hours (PubMed=1659986); 48 hours (Note=In serum-free medium) (PubMed=7540186).

Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.

Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.

Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA58).

Omics: Transcriptome analysis by microarray.

Anecdotal: Nexia, a former Canadian biotech company was orignally developed to make lactose-free free milk using MAC-T.

Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911.

Cell type: Mammary alveolar epithelial cell; CL=CL_0002325.

Species of origin Bos taurus (Bovine) (NCBI Taxonomy: 9913)

Breed/subspecies: Holstein; VBO=VBO_0000237.

Huynh H.T.

Establishment of bovine mammary epithelial cell lines: an in vitro model for lactation.

Thesis MSc (1990); McGill University Montreal; Montreal; Canada

 

PubMed=1659986; DOI=10.1016/0014-4827(91)90422-Q

Huynh H.T., Robitaille G., Turner J.D.

Establishment of bovine mammary epithelial cells (MAC-T): an in vitro model for bovine lactation.

Exp. Cell Res. 197:191-199(1991)

 

PubMed=1423654; DOI=10.1016/S0309-1651(06)80052-4

Rejman J.J., Oliver S.P., Muenchen R.A., Turner J.D.

Proliferation of the MAC-T bovine mammary epithelial cell line in the presence of mammary secretion whey proteins.

Cell Biol. Int. Rep. 16:993-1001(1992)

 

Patent=US5227301

Turner J.D., Huynh H.T.

Immortalized bovine mammary epithelial cell line.

Patent number US5227301, 13-Jul-1993

 

Patent=US5455164

Turner J.D.

Ruminant immortalized mammary epithelial cell lines.

Patent number US5455164, 03-Oct-1995

 

PubMed=7540186; DOI=10.3168/jds.S0022-0302(95)76662-0

Zavizion B., Gorewit R.C., Politis I.

Subcloning the MAC-T bovine mammary epithelial cell line: morphology, growth properties, and cytogenetic analysis of clonal cells.

J. Dairy Sci. 78:515-527(1995)

 

PubMed=14550508; DOI=10.1016/S0739-7240(03)00062-6

Berry S.D.K., Weber Nielsen M.S., Sejrsen K., Pearson R.E., Boyle P.L., Akers R.M.

Use of an immortalized bovine mammary epithelial cell line (MAC-T) to measure the mitogenic activity of extracts from heifer mammary tissue: effects of nutrition and ovariectomy.

Domest. Anim. Endocrinol. 25:245-253(2003)

 

Hosseini A., Sharma R., Bionaz M., Loor J.J.

Transcriptomics comparisons of Mac-T cells versus mammary tissue during late pregnancy and peak lactation.

Adv. Dairy Res. 1:103-103(2013)

 

PubMed=26659725; DOI=10.1152/ajpcell.00261.2015

Arevalo Turrubiarte M., Perruchot M.-H., Finot L., Mayeur F., Dessauge F.

Phenotypic and functional characterization of two bovine mammary epithelial cell lines in 2D and 3D models.

Am. J. Physiol. Cell Physiol. 310:C348-C356(2016)

 

PubMed=30055215; DOI=10.1016/j.virusres.2018.07.013

Martinez Cuesta L., Nieto Farias M.V., Lendez P.A., Barone L., Perez S.E., Dolcini G.L., Ceriani M.C.

Stable infection of a bovine mammary epithelial cell line (MAC-T) with bovine leukemia virus (BLV).

Virus Res. 256:11-16(2018)