NCI-H82

NCI-H82/NCI-H82细胞系/NCI-H82细胞株/NCI-H82人小细胞株肺癌细胞

Cell line name NCI-H82

Synonyms NCI-H-82; H82; H-82; NCI H82; NCIH82; H82sclc

Accession CVCL_1591

Resource Identification Initiative To cite this cell line use: NCI-H82 (RRID:CVCL_1591)

Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: COSMIC cell lines project.

Part of: MYC genetic alteration cell panel (ATCC TCP-1035).

Population: Caucasian.

Doubling time: 24 hours (PubMed=25984343); ~25 hours (CLS=300442); ~30 hours (DSMZ=ACC-556).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Array-based CGH.

Omics: Deep exome analysis.

Omics: Deep proteome analysis.

Omics: Deep quantitative proteome analysis.

Omics: DNA methylation analysis.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.

Sequence variations

Gene fusion; HGNC; 7577; MYH7 + HGNC; 9709; PVT1; Name(s)=PVT1-MYH7 (PubMed=23716474).

Mutation; HGNC; 11998; TP53; Simple; p.Thr125Thr (c.375G>T); ClinVar=VCV000237948; Zygosity=Unspecified; Note=Impairs TP53 splicing dramatically (PubMed=1312696; PubMed=1565469; PubMed=20557307; PubMed=24700732).

HLA typing Source: PubMed=25960936

Class I

HLA-A A*01:01,02:01

HLA-B B*58,08

HLA-C C*07:01,01:02

Class II

HLA-DQ DQA1*04:01,04:01

DQB1*04:02,02:02

HLA-DR DRB1*11:08,11:08

Genome ancestry Source: PubMed=30894373

 

Origin % genome

African 0.24

Native American 0

East Asian, North 1.21

East Asian, South 0

South Asian 0

European, North 78.65

European, South 19.9

Disease Lung small cell carcinoma (NCIt: C4917)

Small cell lung cancer (ORDO: Orphanet_70573)

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy Children:

CVCL_RS66 (H82R) CVCL_WM70 (NCI-H82-A)

Sex of cell Male

Age at sampling 41Y

Category Cancer cell line

STR profile Source(s): ATCC=HTB-175; CLS=300442; Cosmic-CLP=688031; DSMZ=ACC-556; PubMed=25877200

 

Markers:

Amelogenin X

CSF1PO 11

D2S1338 17,24

D3S1358 17

D5S818 12

D7S820 10,13

D8S1179 13

D13S317 8

D16S539 12

D18S51 14,18

D19S433 13

D21S11 28,30

FGA 24,25

Penta D 10,12

Penta E 11,12

TH01 9,9.3

TPOX 11

vWA 14

 

Run an STR similarity search on this cell line

Publications

PubMed=6270685; DOI=10.1073/pnas.78.7.4591; PMCID=PMC319839

Cuttitta F., Rosen S.T., Gazdar A.F., Minna J.D.

Monoclonal antibodies that demonstrate specificity for several types of human lung cancer.

Proc. Natl. Acad. Sci. U.S.A. 78:4591-4595(1981)

 

PubMed=6148444; DOI=10.1093/jnci/73.4.801

Morstyn G., Russo A., Carney D.N., Karawya E., Wilson S.H. Jr., Mitchell J.B.

Heterogeneity in the radiation survival curves and biochemical properties of human lung cancer cell lines.

J. Natl. Cancer Inst. 73:801-807(1984)

 

PubMed=6713399

Rosen S.T., Mulshine J.L., Cuttitta F., Fedorko J., Carney D.N., Gazdar A.F., Minna J.D.

Analysis of human small cell lung cancer differentiation antigens using a panel of rat monoclonal antibodies.

Cancer Res. 44:2052-2061(1984)

 

PubMed=2985257

Carney D.N., Gazdar A.F., Bepler G., Guccion J.G., Marangos P.J., Moody T.W., Zweig M.H., Minna J.D.

Establishment and identification of small cell lung cancer cell lines having classic and variant features.

Cancer Res. 45:2913-2923(1985)

 

PubMed=2985258

Gazdar A.F., Carney D.N., Nau M.M., Minna J.D.

Characterization of variant subclasses of cell lines derived from small cell lung cancer having distinctive biochemical, morphological, and growth properties.

Cancer Res. 45:2924-2930(1985)

 

PubMed=2986243; DOI=10.1007/978-3-642-82372-5_4

Whang-Peng J., Lee E.C.

Cytogenetics of human small cell lung cancer.

Recent Results Cancer Res. 97:37-46(1985)

 

PubMed=2473086; DOI=10.1242/jcs.91.1.91

Broers J.L.V., Rot M.K., Oostendorp T., Bepler G., de Leij L.F.M.H., Carney D.N., Vooijs G.P., Ramaekers F.C.S.

Spontaneous changes in intermediate filament protein expression patterns in lung cancer cell lines.

J. Cell Sci. 91:91-108(1988)

 

PubMed=3335022

Alley M.C., Scudiero D.A., Monks A., Hursey M.L., Czerwinski M.J., Fine D.L., Abbott B.J., Mayo J.G., Shoemaker R.H., Boyd M.R.

Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.

Cancer Res. 48:589-601(1988)

 

PubMed=2388294; DOI=10.1093/jnci/82.17.1420

McLemore T.L., Litterst C.L., Coudert B.P., Liu M.C., Hubbard W.C., Adelberg S., Czerwinski M.J., McMahon N.A., Eggleston J.C., Boyd M.R.

Metabolic activation of 4-ipomeanol in human lung, primary pulmonary carcinomas, and established human pulmonary carcinoma cell lines.

J. Natl. Cancer Inst. 82:1420-1426(1990)

 

PubMed=1845952; DOI=10.1002/1097-0142(19910201)67:3<619::AID-CNCR2820670317>3.0.CO;2-Y

Broers J.L.V., Mijnheere E.P., Rot M.K., Schaart G., Sijlmans A., Boerman O.C., Ramaekers F.C.S.

Novel antigens characteristic of neuroendocrine malignancies.

Cancer 67:619-633(1991)

 

PubMed=1312696

D'Amico D., Carbone D.P., Mitsudomi T., Nau M.M., Fedorko J., Russell E., Johnson B., Buchhagen D.L., Bodner S.M., Phelps R.M., Gazdar A.F., Minna J.D.

High frequency of somatically acquired p53 mutations in small-cell lung cancer cell lines and tumors.

Oncogene 7:339-346(1992)

 

PubMed=1563005

Giaccone G., Battey J., Gazdar A.F., Oie H.K., Draoui M., Moody T.W.

Neuromedin B is present in lung cancer cell lines.

Cancer Res. 52:2732s-2736s(1992)

 

PubMed=1565469

Bodner S.M., Minna J.D., Jensen S.M., D'Amico D., Carbone D.P., Mitsudomi T., Fedorko J., Buchhagen D.L., Nau M.M., Gazdar A.F., Linnoila R.I.

Expression of mutant p53 proteins in lung cancer correlates with the class of p53 gene mutation.

Oncogene 7:743-749(1992)

 

PubMed=7720728

Senden N.H.M., van de Velde H.J.K., Broers J.L.V., Timmer E.D.J., Kuijpers H.J.H., Roebroek A.J.M., van de Ven W.J.M., Ramaekers F.C.S.

Subcellular localization and supramolecular organization of neuroendocrine-specific protein B (NSP-B) in small cell lung cancer.

Eur. J. Cell Biol. 65:341-353(1994)

 

PubMed=8626706; DOI=10.1074/jbc.271.19.11477

Quinn K.A., Treston A.M., Unsworth E.J., Miller M.-J., Vos M., Grimley C., Battey J., Mulshine J.L., Cuttitta F.

Insulin-like growth factor expression in human cancer cell lines.

J. Biol. Chem. 271:11477-11483(1996)