2V6.11

2V6.11/2V6.11细胞系/2V6.11细胞株/2V6.11Adenovirus 5转化胚肾细胞

Cell line name    2V6.11
Accession    CVCL_6355
Resource Identification Initiative    To cite this cell line use: 2V6.11 (RRID:CVCL_6355)
Comments    Characteristics: Adenovirus 5 E4 34kda protein is expressed under the control of an ecdysone-inducible promoter (PubMed=15141036).
Characteristics: Transfected with the pVgRXR vector that contains the VgEcR fusion protein under the control of a CMV promoter and RXRA under the control of a RSV promoter as well as BleoR (PubMed=15141036).
Genetic integration: Method=Transfection; Gene=HGNC; 10477; RXRA.
Genetic integration: Method=Transfection; Gene=FlyBase_Gene; FBgn0000546; EcR (Note=With p.Met1_Val221del, as 2nd part of VgECR).
Genetic integration: Method=Transfection; Gene=UniProtKB; Q6VGT3; Adenovirus 5 E4 34kda protein.
Genetic integration: Method=Transfection; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Thr412del, as 1st part of VgECR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin    Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy    Parent: CVCL_E3D4 (293VgRXR)
Sex of cell    Female
Age at sampling    Fetus
Category    Transformed cell line
STR profile    Source(s): ATCC=CRL-2784; CLS=305147

Markers:
Amelogenin    X
CSF1PO    7,11,12
D2S1338    19
D3S1358    15,17
D5S818    8,9
D6S1043    11
D7S820    11
D8S1179    12,14
D12S391    19,21
D13S317    12,14
D16S539    9,13
D18S51    17,19
D19S433    15,18
D21S11    28,30.2
FGA    23
Penta D    9,10
Penta E    7,15
TH01    7,9.3
TPOX    11
vWA    16,19

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Publications    
PubMed=15141036; DOI=10.1093/nar/gkh593; PMCID=PMC419473
Mohammadi E.S., Ketner E.A., Johns D.C., Ketner G.
Expression of the adenovirus E4 34k oncoprotein inhibits repair of double strand breaks in the cellular genome of a 293-based inducible cell line.
Nucleic Acids Res. 32:2652-2659(2004)