HBL-100

HBL-100/HBL-100细胞系/HBL-100细胞株/HBL-100人整合SV40基因的乳腺上皮细胞

Cell line name HBL-100

Synonyms HBL 100; HBL100

Accession CVCL_4362

Resource Identification Initiative To cite this cell line use: HBL-100 (RRID:CVCL_4362)

Comments Problematic cell line: Misidentified. Presence of a Y chromosome in cell line that was thought to be of female origin (PubMed=16643607; PubMed=20143388). Originally thought to originate from a casein-producing breast cell line from a 27 year old Caucasian woman. In addition contains SV40 genomic sequence while the cell line was deemed to be spontaneously immortalized.

Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: JWGray breast cancer cell line panel.

Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00207.

Virology: Contains 2 copies of the SV40 genome, one complete the other incomplete tandemly integrated in the cell line genome.

Doubling time: 28 hours (Note=At 10th passage) (PubMed=6891286); 1.5 days (PubMed=9671407); ~40 hours (CLS=300178); 30.21 hours (JWGray panel).

Karyotypic information: Near triploid with the modal number of 67 chromosomes (ATCC=HTB-124).

Microsatellite instability: Stable (MSS) (PubMed=12661003).

Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).

Omics: Array-based CGH.

Omics: Deep exome analysis.

Omics: DNA methylation analysis.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

HLA typing Source: PubMed=26589293

Class I

HLA-A A*01:01,02:01

HLA-B B*08:01,40:01

HLA-C C*03:04,07:01

Class II

HLA-DR DRB1*14:76,15:01

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy Children:

CVCL_A5RV (HBLT-11) CVCL_A5RW (HBLT-12) CVCL_A5RX (HBLT-13)

Sex of cell Male

Age at sampling Age unspecified

Category Transformed cell line

STR profile Source(s): ATCC=HTB-124; CCRID; CLS=300178; PubMed=11416159; PubMed=25877200; PubMed=28889351

 

Markers:

Amelogenin X (CCRID; PubMed=25877200; PubMed=28889351)

X,Y (ATCC=HTB-124; CLS=300178; PubMed=11416159)

CSF1PO 10

D2S1338 18,24

D3S1358 14,16

D5S818 11,12

D7S820 8,12

D8S1179 12,15

D13S317 12

D16S539 9,12

D18S51 16

D19S433 15

D21S11 28,30

FGA 25

Penta D 12

Penta E 7

TH01 6,8

TPOX 8

vWA 16

 

Run an STR similarity search on this cell line

Publications

CLPUB00368

Polanowski F.P., Gaffney E.V., Burke R.E.

HBL-100, a cell line established from human breast milk.

In Vitro 12:328-328(1976)

 

PubMed=6891286; DOI=10.1007/BF00204786

Gaffney E.V.

A cell line (HBL-100) established from human breast milk.

Cell Tissue Res. 227:563-568(1982)

 

PubMed=6500159; DOI=10.1159/000163283

Gershwin M.E., Lentz D., Owens R.B.

Relationship between karyotype of tissue culture lines and tumorigenicity in nude mice.

Exp. Cell Biol. 52:361-370(1984)

 

PubMed=2995096; DOI=10.1016/0014-4827(85)90238-1

Caron de Fromentel C., Nardeux P.-C., Soussi T., Lavialle C., Estrade S., Carloni G., Chandrasekaran K., Cassingena R.

Epithelial HBL-100 cell line derived from milk of an apparently healthy woman harbours SV40 genetic information.

Exp. Cell Res. 160:83-94(1985)

 

PubMed=3109929; DOI=10.1016/0014-4827(87)90318-1

Dealtry G.B., Naylor M.S., Fiers W.C., Balkwill F.R.

The effect of recombinant human tumour necrosis factor on growth and macromolecular synthesis of human epithelial cells.

Exp. Cell Res. 170:428-438(1987)

 

PubMed=2840847

Marlhens F., Saint-Ruf C., Nardeux P.-C., Lavialle C., Brouty-Boye D., Dutrillaux B., Cassingena R.

Karyotype evolution of the human HBL-100 cell line and mapping of the integration site of SV40 DNA.

Ann. Genet. 31:81-86(1988)

 

PubMed=2547730; DOI=10.1002/ijc.2910440230

Saint-Ruf C., Nardeux P.-C., Cebrian J., Lacasa M., Lavialle C., Cassingena R.

Molecular cloning and characterization of endogenous SV40 DNA from human HBL-100 cells.

Int. J. Cancer 44:367-372(1989)

 

DOI=10.1016/B978-0-12-333530-2.50009-5

Leibovitz A.

Cell lines from human breast.

(In book chapter) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.161-184; Academic Press; New York; USA (1994)

 

PubMed=9671407; DOI=10.1038/sj.onc.1201814

Sweeney K.J., Swarbrick A., Sutherland R.L., Musgrove E.A.

Lack of relationship between CDK activity and G1 cyclin expression in breast cancer cells.

Oncogene 16:2865-2878(1998)

 

PubMed=10969801

Forozan F., Mahlamaki E.H., Monni O., Chen Y.-D., Veldman R., Jiang Y., Gooden G.C., Ethier S.P., Kallioniemi A.H., Kallioniemi O.-P.

Comparative genomic hybridization analysis of 38 breast cancer cell lines: a basis for interpreting complementary DNA microarray data.

Cancer Res. 60:4519-4525(2000)

 

PubMed=11416159; DOI=10.1073/pnas.121616198; PMCID=PMC35459

Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.

Short tandem repeat profiling provides an international reference standard for human cell lines.

Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)