U-937

U-937/U-937细胞系/U-937细胞株/U-937人急性单核细胞白血病

Cell line name U-937

Synonyms U937; U 937

Accession CVCL_0007

Resource Identification Initiative To cite this cell line use: U-937 (RRID:CVCL_0007)

Comments Group: Space-flown cell line (cellonaut).

Problematic cell line: Partially contaminated. Some ATCC stocks were cross-contaminated by K-562 initially (PubMed=7759961). The problem was corrected and subsequent stocks have been confirmed to carry only U-937.

Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: LL-100 blood cancer cell line panel.

Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00168.

Population: Caucasian.

Characteristics: Can be induced to differentiate into macrophages by several factors such as phorbol ester (PMA) and 12-O-tetradecanoyl-13-phorbol acetate (TPA).

Doubling time: 95-100 hours (PubMed=178611); ~30-40 hours (DSMZ=ACC-5).

Karyotypic information: Has lost chromosome Y.

Microsatellite instability: Stable (MSS) (PubMed=10739008; PubMed=11226526).

Omics: Deep antibody staining analysis.

Omics: Deep exome analysis.

Omics: Deep proteome analysis.

Omics: Deep quantitative proteome analysis.

Omics: DNA methylation analysis.

Omics: Proteome analysis by 2D-DE/MS.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Anecdotal: Have been flown in space on shuttle flight STS-76 and on Shenzhou-8 to study growth and cytoskeletal changes in microgravity (PubMed=10352142; DOI=10.1016/j.actaastro.2013.06.007).

Derived from site: In situ; Pleural effusion; UBERON=UBERON_0000175.

PubMed=6582512; DOI=10.1073/pnas.81.2.568; PMCID=PMC344720

Mattes M.J., Cordon-Cardo C., Lewis J.L. Jr., Old L.J., Lloyd K.O.

Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.

Proc. Natl. Acad. Sci. U.S.A. 81:568-572(1984)

 

PubMed=2985879; DOI=10.1016/0145-2126(85)90084-0

Drexler H.G., Gaedicke G., Minowada J.

Isoenzyme studies in human leukemia-lymphoma cell lines -- 1 carboxylic esterase.

Leuk. Res. 9:209-229(1985)

 

PubMed=3159941; DOI=10.1016/0145-2126(85)90134-1

Drexler H.G., Gaedicke G., Minowada J.

Isoenzyme studies in human leukemia-lymphoma cell lines -- III Beta-hexosaminidase (E.C. 3.2.1.30).

Leuk. Res. 9:549-559(1985)

 

PubMed=3874327; DOI=10.1016/0145-2126(85)90133-x

Drexler H.G., Gaedicke G., Minowada J.

Isoenzyme studies in human leukemia-lymphoma cells lines -- II. Acid phosphatase.

Leuk. Res. 9:537-548(1985)

 

DOI=10.1159/000415060

Morikawa S., Harada T., Katoh T.

Heterogeneity of cellular origins in human malignant lymphoma cell line derived from histo-monocytic lineage cells.

(In book chapter) Cellular, molecular, genetic approaches to immunodiagnosis and immunotherapy. 8th International conference on labeled antibodies, Tokyo, November 1985; Kano K., Mori S., Sugisaki T., Torisu M. (eds.); pp.373-380; Karger; Basel; Switzerland (1988)

 

PubMed=2140233; DOI=10.1111/j.1440-1827.1990.tb01549.x

Nakano A., Harada T., Morikawa S., Kato Y.

Expression of leukocyte common antigen (CD45) on various human leukemia/lymphoma cell lines.

Acta Pathol. Jpn. 40:107-115(1990)

 

PubMed=2243057; DOI=10.1007/BF02624468

Kong Z.-L., Miwa M., Murakami H., Shinohara K.

Establishment of a macrophagelike cell line derived from U-937, human histiocytic lymphoma, grown serum-free.

In Vitro Cell. Dev. Biol. 26:949-954(1990)

 

DOI=10.1007/978-94-011-3550-4_20

Shinohara K., Kong Z.-L., Murakami H.

Characterization of a human derived macrophage-like cell line, U-M.

(In book chapter) Animal cell culture and production of biologicals; Sasaki R., Ikura K. (eds.); pp.181-186; Springer; Dordrecht; Netherlands (1991)

 

PubMed=1913629; DOI=10.1016/0304-3835(91)90051-I

Chang K.S.-S., Liu W.-T., Josephs S.F.

Regulation of cellular trans-activating activities in two different promonocytic leukemia cell lines.

Cancer Lett. 60:75-83(1991)

 

PubMed=1571549; DOI=10.1182/blood.V79.9.2378.2378

Sugimoto K., Toyoshima H., Sakai R., Miyagawa K., Hagiwara K., Ishikawa F., Takaku F., Yazaki Y., Hirai H.

Frequent mutations in the p53 gene in human myeloid leukemia cell lines.

Blood 79:2378-2383(1992)

 

PubMed=8300570; DOI=10.1016/S0021-9258(17)41964-8

Rubin E.H., Pantazis P., Bharti A., Toppmeyer D., Giovanella B.C., Kufe D.W.

Identification of a mutant human topoisomerase I with intact catalytic activity and resistance to 9-nitro-camptothecin.

J. Biol. Chem. 269:2433-2439(1994)

 

PubMed=7759961; DOI=10.1002/jlb.57.5.804

Reid Y.A., McGuire L., O'Neill K., Macy M.L., Chen T.-R., McClintock P.R., Dorotinsky C.S., Hay R.

Cell line cross-contamination of U-937.

J. Leukoc. Biol. 57:804-804(1995)

 

PubMed=8547074; DOI=10.1111/j.1365-2141.1995.tb05302.x

Siebert R., Willers C.P., Schramm A., Fossa A., Dresen I.M.G., Uppenkamp M.J., Nowrousian M.R., Seeber S., Opalka B.

Homozygous loss of the MTS1/p16 and MTS2/p15 genes in lymphoma and lymphoblastic leukaemia cell lines.

Br. J. Haematol. 91:350-354(1995)

 

PubMed=8558913

Morita S., Tsuchiya S., Fujie H., Itano M., Ohashi Y., Minegishi M., Imaizumi M., Endo M., Takano N., Konno T.

Cell surface c-kit receptors in human leukemia cell lines and pediatric leukemia: selective preservation of c-kit expression on megakaryoblastic cell lines during adaptation to in vitro culture.

Leukemia 10:102-105(1996)

 

PubMed=8558920

Dirks W.G., Zaborski M., Jager K., Challier C., Shiota M., Quentmeier H., Drexler H.G.

The (2;5)(p23;q35) translocation in cell lines derived from malignant lymphomas: absence of t(2;5) in Hodgkin-analogous cell lines.

Leukemia 10:142-149(1996)

 

PubMed=8643484; DOI=10.1073/pnas.93.10.4804; PMCID=PMC39360

Dreyling M.H., Martinez-Climent J.A., Zheng M., Mao J., Rowley J.D., Bohlander S.K.

The t(10;11)(p13;q14) in the U937 cell line results in the fusion of the AF10 gene and CALM, encoding a new member of the AP-3 clathrin assembly protein family.

Proc. Natl. Acad. Sci. U.S.A. 93:4804-4809(1996)