BT474

BT474/BT474细胞系/BT474细胞株/BT474人乳腺癌细胞Cell line name BT-474

Synonyms Bt-474; BT474

Accession CVCL_0179

Resource Identification Initiative To cite this cell line use: BT-474 (RRID:CVCL_0179)

Comments Part of: AKT genetic alteration cell panel (ATCC TCP-1029).

Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: COSMIC cell lines project.

Part of: EGFR genetic alteration cell panel (ATCC TCP-1027).

Part of: ERK genetic alteration cell panel (ATCC TCP-1033).

Part of: JWGray breast cancer cell line panel.

Part of: ICBP43 breast cancer cell line panel.

Part of: KuDOS 95 cell line panel.

Part of: MD Anderson Cell Lines Project.

Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).

Population: Caucasian.

Doubling time: 3.5 days (PubMed=9671407); 78 hours (PubMed=25984343); 45.8 hours (PubMed=24389870); ~60-80 hours (CLS=300131); ~100 hours (DSMZ=ACC-64); 92.49 hours (JWGray panel).

Microsatellite instability: Stable (MSS) (PubMed=23671654; Sanger).

Omics: Array-based CGH.

Omics: CNV analysis.

Omics: Deep exome analysis.

Omics: Deep phosphoproteome analysis.

Omics: Deep proteome analysis.

Omics: Deep quantitative proteome analysis.

Omics: DNA methylation analysis.

Omics: Genome sequenced.

Omics: Glycoproteome analysis by proteomics.

Omics: Metabolome analysis.

Omics: miRNA expression profiling.

Omics: Protein expression by reverse-phase protein arrays.

Omics: shRNA library screening.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Anecdotal: Used in a study utilising the fruit fly's olfactory system to detect cancer cells (PubMed=24389870).

Derived from site: In situ; Breast; UBERON=UBERON_0000310.

PubMed=7842014; DOI=10.1038/ng1094-155

Guan X.-Y., Meltzer P.S., Dalton W.S., Trent J.M.

Identification of cryptic sites of DNA sequence amplification in human breast cancer by chromosome microdissection.

Nat. Genet. 8:155-161(1994)

 

PubMed=9671407; DOI=10.1038/sj.onc.1201814

Sweeney K.J., Swarbrick A., Sutherland R.L., Musgrove E.A.

Lack of relationship between CDK activity and G1 cyclin expression in breast cancer cells.

Oncogene 16:2865-2878(1998)

 

PubMed=10862037; DOI=10.1002/1098-2264(200007)28:3<308::AID-GCC9>3.0.CO;2-B

Kytola S., Rummukainen J., Nordgren A., Karhu R., Farnebo F., Isola J.J., Larsson C.

Chromosomal alterations in 15 breast cancer cell lines by comparative genomic hybridization and spectral karyotyping.

Genes Chromosomes Cancer 28:308-317(2000)

 

PubMed=10969801

Forozan F., Mahlamaki E.H., Monni O., Chen Y.-D., Veldman R., Jiang Y., Gooden G.C., Ethier S.P., Kallioniemi A.H., Kallioniemi O.-P.

Comparative genomic hybridization analysis of 38 breast cancer cell lines: a basis for interpreting complementary DNA microarray data.

Cancer Res. 60:4519-4525(2000)

 

PubMed=11343771; DOI=10.1016/S0165-4608(00)00387-3

Rummukainen J., Kytola S., Karhu R., Farnebo F., Larsson C., Isola J.J.

Aberrations of chromosome 8 in 16 breast cancer cell lines by comparative genomic hybridization, fluorescence in situ hybridization, and spectral karyotyping.

Cancer Genet. Cytogenet. 126:1-7(2001)

 

PubMed=11687795; DOI=10.1038/ng754

Snijders A.M., Nowak N.J., Segraves R., Blackwood S., Brown N., Conroy J., Hamilton G., Hindle A.K., Huey B., Kimura K., Law S., Myambo K., Palmer J., Ylstra B., Yue J.P., Gray J.W., Jain A.N., Pinkel D., Albertson D.G.

Assembly of microarrays for genome-wide measurement of DNA copy number.

Nat. Genet. 29:263-264(2001)

 

PubMed=12353263; DOI=10.1002/gcc.10107

Popovici C., Basset C., Bertucci F., Orsetti B., Adelaide J., Mozziconacci M.-J., Conte N., Murati A., Ginestier C., Charafe-Jauffret E., Ethier S.P., Lafage-Pochitaloff M., Theillet C., Birnbaum D., Chaffanet M.

Reciprocal translocations in breast tumor cell lines: cloning of a t(3;20) that targets the FHIT gene.

Genes Chromosomes Cancer 35:204-218(2002)

 

PubMed=12800145; DOI=10.1002/gcc.10218

Adelaide J., Huang H.-E., Murati A., Alsop A.E., Orsetti B., Mozziconacci M.-J., Popovici C., Ginestier C., Letessier A., Basset C., Courtay-Cahen C., Jacquemier J., Theillet C., Birnbaum D., Edwards P.A.W., Chaffanet M.

A recurrent chromosome translocation breakpoint in breast and pancreatic cancer cell lines targets the neuregulin/NRG1 gene.

Genes Chromosomes Cancer 37:333-345(2003)

 

PubMed=16142302; DOI=10.3892/ijo.27.4.881

de Longueville F., Lacroix M., Barbuto A.-M., Bertholet V., Gallo D., Larsimont D., Marcq L., Zammatteo N., Boffe S., Leclercq G., Remacle J.

Molecular characterization of breast cancer cell lines by a low-density microarray.

Int. J. Oncol. 27:881-892(2005)

 

PubMed=16397213; DOI=10.1158/0008-5472.CAN-05-2853

Elstrodt F., Hollestelle A., Nagel J.H.A., Gorin M., Wasielewski M., van den Ouweland A.M.W., Merajver S.D., Ethier S.P., Schutte M.

BRCA1 mutation analysis of 41 human breast cancer cell lines reveals three new deleterious mutants.

Cancer Res. 66:41-45(2006)

 

PubMed=16195238; DOI=10.1093/carcin/bgi231

Hussain-Hakimjee E.A., Peng X.-J., Mehta R.R., Mehta R.G.

Growth inhibition of carcinogen-transformed MCF-12F breast epithelial cells and hormone-sensitive BT-474 breast cancer cells by 1alpha-hydroxyvitamin D5.

Carcinogenesis 27:551-559(2006)

 

PubMed=16417655; DOI=10.1186/bcr1370; PMCID=PMC1413994

Shadeo A., Lam W.L.

Comprehensive copy number profiles of breast cancer cell model genomes.

Breast Cancer Res. 8:R9.1-R9.14(2006)

 

PubMed=16541312; DOI=10.1007/s10549-006-9186-z

Wasielewski M., Elstrodt F., Klijn J.G.M., Berns E.M.J.J., Schutte M.

Thirteen new p53 gene mutants identified among 41 human breast cancer cell lines.

Breast Cancer Res. Treat. 99:97-101(2006)