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- 详细信息
- 文献和实验
- 技术资料
- 库存:
1000
- 英文名:
pFastBac Dual
- 保质期:
3年
- 供应商:
百欧博伟
- 保存条件:
-80
平台编号:bio-109260
复制子: pUC ori,F1 ori
终止子: SV40 poly(A) signal
质粒大小: 5238bp
原核抗性: Amp
筛选标记: Gen
克隆菌株: DH5α
培养条件: 37℃,有氧 LB
5'测序引物: pFastbac-F: TATTCCGGATTATTCATACC
3'测序引物: pFastBac-R: ACAAATGTGGTATGGCTGA
质粒简介
pFastBac Dual昆虫细胞表达载体包含有两个启动子,能够同时表达两个外源基因,一个表达基因是polyhedrin (PH)启动子控制,另一个外源基因是由p10启动子控制。
pFastBacDual 载体的特点是在单个载体上有两个启动子,用于在使用 Bac-to-Bac 杆状病毒表达系统(Bac-to-Bac Baculovirus Expression System) 时在昆虫细胞中同时表达两个蛋白质。该载体具有两个强启动子,多角体蛋白启动子和p10 启动子,因此可实现高水平表达。包含 CAT和β-葡萄糖醛酸酶 (gus) 的表达对照。
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文献和实验Dual- and Triple-Color Fluorospot
different Ig isotypes (6) and later for analysis of cytokines (7). Although successfully used in several studies (8, 9), the dual ELISPOT technique can be technically challenging to perform, suffers from several inherent analytical difficulties
system, or interaction trap, is one of the most versatile methods available with which to identify and establish protein?protein interactions. The dual bait system is one adaptation of the classic approach. This system facilitates the simultaneous comparison
It is sometimes desirable to extract both RNA and DNA from the same sample, especially when the sample is small. This can be achieved by isolating a total nucleic acid fraction that is then divided into two portions, which are treated
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