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Thiol-Modified Hyaluronan and Gelatin Hydrogel Kit
Advanced Biomatrix HyStem透明质酸水凝胶
GS313F
HyStem®-C
Thiol-Modified Hyaluronan and Gelatin Hydrogel Kit
Catalog #GS312F #GS313F #GS1005F
Product Description
HyStem®-C Hydrogel Kits - The starter matrix.
HyStem®-C hydrogels provide an excellent starting point for optimizing the matrix for cell culture. HyStem-C is fully
chemically-defined and based on three biocompatible components: thiol-modified hyaluronan (Glycosil), thiol-reactive
crosslinker, PEGDA (Extralink), and thiol-modified denatured collagen (Gelin-S®). Gelin-S provides basic cell-attachment
sites for a wide variety of primary cells and cell lines and is therefore recommended as an ideal substrate for adherent cell
types and for cell culture optimization. In some cases, HyStem-C hydrogels can be further enhanced by the addition of ECM
proteins to match native signals.
Product may be shipped ambient, but please store components at the temperature stated on the vials until ready for use.
Features
Hydrogels are suitable for culturing primary cells, stem cells and cell lines.
Cells can be encapsulated or grown on the hydrogel surface in any format, including culture flasks, 6- to 384-well plates or
tissue culture inserts.
Hydrogels can be easily customized by the user to possess the desired stiffness and gelation time by manipulating component
concentration and mixing ratios.
Customizable gelation properties including gelation time and hydrogel stiffness.
This crosslinker provides the same advantages offered by Extralink with the additional benefit of containing
easily reducible internal bonds. This allows for fast, easy recovery of single cells or clusters from the hydrogel for applications
like RNA analysis or flow cytometry instead of slow enzymatic methods that can impact cell viability.
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文献和实验培养。 1. 天然ECM作为支持材料的3D培养这种方法以天然ECM作为支持材料,根据培养细胞类型,优化3D培养基质配方,以满足不同组织细胞的培养需求。很多公司都可提供细胞外基质产品用于3D培养,例如TAP Biosystems(已被Sartorius Stedim Biotech收购)的RAFT胶原系统,Matrigel模拟基底膜基质产品;Advanced BioMatrix,Amsbio和Sigma-Aldrich公司也都有细胞外基质凝胶产品。但天然基质材料存在一定病原风险,且材料可能存在批次差别性等缺点
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