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T25
YAC-1/YAC-1细胞系/YAC-1细胞株/YAC-1小鼠淋巴瘤细胞(NK靶细胞
Cell line name YAC-1
Synonyms Yac-1; YAC
Accession CVCL_2244
Resource Identification Initiative To cite this cell line use: YAC-1 (RRID:CVCL_2244)
Comments Part of: Tumor Immunology Bank (TIB) collection from Salk (transferred to ATCC in 1981).
Doubling time: ~20 hours (DSMZ=ACC-96).
Transformant: NCBI_TaxID; 11801; Moloney murine leukemia virus (MoMuLV).
Derived from site: In situ; Ascites; UBERON=UBERON_0007795.
Disease Mouse lymphoma (NCIt: C21602)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: A/Sn.
Category Cancer cell line
STR profile Source(s): PubMed=31220119
Markers:
Mouse STR 1-1 11
Mouse STR 1-2 17,18
Mouse STR 2-1 16
Mouse STR 3-2 14
Mouse STR 4-2 21.3
Mouse STR 5-5 16
Mouse STR 6-4 18
Mouse STR 6-7 12
Mouse STR 7-1 25.2
Mouse STR 8-1 16
Mouse STR 11-2 15
Mouse STR 12-1 16
Mouse STR 13-1 16.2,17.2
Mouse STR 15-3 22.3
Mouse STR 17-2 16
Mouse STR 18-3 18
Mouse STR 19-2 12,13
Mouse STR X-1 26
Publications
PubMed=1234049; DOI=10.1002/eji.1830050208
Kiessling R., Klein E., Wigzell H.
'Natural' killer cells in the mouse. I. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Specificity and distribution according to genotype.
Eur. J. Immunol. 5:112-117(1975)
PubMed=75945; DOI=10.1099/0022-1317-38-3-483
O'Brien S.J., Simonson J.M., Davis S.
Deposition of retrovirus associated antigens (p30 and gp70) on cell membranes of feline and murine leukaemia virus infected cells.
J. Gen. Virol. 38:483-496(1978)
PubMed=31220119; DOI=10.1371/journal.pone.0218412; PMCID=PMC6586308
Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J.-L., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B.A., Gollin S.M., Lewis D.W., Reid Y.A.
Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.
PLoS ONE 14:E0218412-E0218412(2019)
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文献和实验*发表【中文论文】请标注：由上海酶研生物科技有限公司提供；
*发表【英文论文】请标注：From Shanghai EK-Bioscience Biotechnology Co., Ltd.
YAC Transfer by Microinjection
Soon after the first report of how yeast artificial chromosomes (YACs) could be used as cloning vectors for large DNA fragments, the transfer of YACs into mammalian cells came into focus of interest, Following mammalian cell transfer, the YAC
YAC transfer by Kar1 mating: Reference: Hugerat, Y., Spencer, F., Zenvirth, D. and Simchen, G. (1994). A versatile method for efficient YAC transfer between any two strains. Genomics 22, 108-117 2. Spencer, F., Hugerat, Y
Construction and Screening of YAC Libraries
The discovery by Burke et al. in 1987 ( 1 ) that yeast can accept large pieces of heterologous DNA as yeast artificial chromosomes (YAC) marked a milestone in the analysis of complex genomes. While standard prokaryotic
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