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T25
bend.3/bend.3细胞系/bend.3细胞株/bend.3小鼠脑微血管内皮细胞株
Cell line name bEnd.3
Synonyms bEND.3; b.End3; Bend.3; bEnd3; BEND-3; BEND3; brain-derived Endothelial cells.3
Accession CVCL_0170
Resource Identification Initiative To cite this cell line use: bEnd.3 (RRID:CVCL_0170)
Comments Characteristics: Widely used for blood vascular research.
Doubling time: 26-30 hours (PubMed=14568334).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P03077; Murine polyomavirus (strain A2) (MPyV) middle T antigen (PyMT).
Transformant: NCBI_TaxID; 10636; Murine polyomavirus (strain A2) (MPyV).
Derived from site: In situ; Brain; UBERON=UBERON_0000955.
Cell type: Endothelial cell; CL=CL_0000115.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: BALB/c.
Hierarchy Children:
CVCL_E1JC (HyCyte bEnd.3 KO-mCdh5)
Sex of cell Sex unspecified
Age at sampling 6W
Category Transformed cell line
PubMed=2379237; DOI=10.1016/0092-8674(90)90009-4
Montesano R., Pepper M.S., Mohle-Steinlein U., Risau W., Wagner E.F., Orci L.
Increased proteolytic activity is responsible for the aberrant morphogenetic behavior of endothelial cells expressing the middle T oncogene.
Cell 62:435-445(1990)
PubMed=14568334; DOI=10.1016/S0006-8993(03)03443-7
Omidi Y., Campbell L., Barar J., Connell D., Akhtar S., Gumbleton M.
Evaluation of the immortalised mouse brain capillary endothelial cell line, b.End3, as an in vitro blood-brain barrier model for drug uptake and transport studies.
Brain Res. 990:95-112(2003)
PubMed=22224974; DOI=10.1089/scd.2011.0501
Joubin K., Richardson A., Novoa N., Tu E., Tomishima M.J.
The endothelial cell line bEnd.3 maintains human pluripotent stem cells.
Stem Cells Dev. 21:2312-2321(2012)
PubMed=25282873; PMCID=PMC4636729
Yuen D., Leu R.K., Tse J., Wang S., Chen L.L., Chen L.
Novel characterization of bEnd.3 cells that express lymphatic vessel endothelial hyaluronan receptor-1.
Lymphology 47:73-81(2014)
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文献和实验*发表【中文论文】请标注：由上海酶研生物科技有限公司提供；
*发表【英文论文】请标注：From Shanghai EK-Bioscience Biotechnology Co., Ltd.
Culture of BEND Cells (Bovine Endometrial Cells)
Culture of BEND Cells (Bovine Endometrial Cells) Charles E. Krininger, III and Peter J. Hansen Dept. of Animal Sciences, University of Florida This protocol was adapted from one prepared by Aydin Guzeloglu and William
Culture of BEND Cells (Bovine Endometrial Cells)
per freezing run to test viability. References using BEND cells Johnson et al. Endocrine 10:243 (1999) Perry et al. Mol. Endocrinol. 13: 1197 (1999) Austin et al. Endocrinology 140: 542 (1999)
细胞治疗是细胞和基因治疗的重要组成部分，它通过使用特定类型的细胞来修复、替换或调节受损的组织和器官。在细胞治疗中，不同类型的细胞因其独特的生物学特性而被广泛应用于多种疾病的治疗。以下是一些常用的细胞类型及其在细胞治疗中的应用。 （1）免疫细胞 免疫细胞是细胞治疗中最重要且研究最多的细胞类型之一，主要包括 T 细胞、自然杀伤细胞（NK 细胞）和树突状细胞（DC 细胞）。 T 细胞：T 细胞是人体免疫系统的核心细胞，具有强大的抗肿瘤能力。CAR-T 细胞疗法是目前最成功的免疫细胞治疗技术
技术资料
