CHO-K1(悬浮）细胞

CHO-K1(悬浮）细胞系、CHO-K1(悬浮）细胞株、CHO-K1(悬浮）细胞、CHO-K1(悬浮）细胞、CHO-K1(悬浮）中国仓鼠卵巢上皮细胞

Cell line name CHO-K1

Synonyms CHO K1; CHOK1; CHO cell clone K1; GM15452

Accession CVCL_0214

Resource Identification Initiative To cite this cell line use: CHO-K1 (RRID:CVCL_0214)

Comments Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-9618.

Doubling time: ~24 hours (DSMZ=ACC-110).

Omics: Genome sequenced.

Omics: Metabolome analysis.

Omics: miRNA expression profiling.

Omics: Deep proteome analysis.

Omics: Glycoproteome analysis by proteomics.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Derived from site: In situ; Ovary; UBERON=UBERON_0000992.

Cell type: Epithelial cell of ovary; CL=CL_2000064.

Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)

PubMed=10320750; DOI=10.1016/S0027-5107(99)00077-9

Hu T., Miller C.M., Ridder G.M., Aardema M.J.

Characterization of p53 in Chinese hamster cell lines CHO-K1, CHO-WBL, and CHL: implications for genotoxicity testing.

Mutat. Res. 426:51-62(1999)

 

PubMed=21804562; DOI=10.1038/nbt.1932; PMCID=PMC3164356

Xu X., Nagarajan H., Lewis N.E., Pan S.-K., Cai Z.-M., Liu X., Chen W.-B., Xie M., Wang W.-L., Hammond S., Andersen M.R., Neff N., Passarelli B., Koh W., Fan H.C., Wang J.-B., Gui Y.-T., Lee K.H., Betenbaugh M.J., Quake S.R., Famili I., Palsson B.O., Wang J.

The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line.

Nat. Biotechnol. 29:735-741(2011)

 

PubMed=21945585; DOI=10.1016/j.jbiotec.2011.09.014

Becker J., Hackl M., Rupp O., Jakobi T., Schneider J., Szczepanowski R., Bekel T., Borth N., Goesmann A., Grillari J., Kaltschmidt C., Noll T., Puhler A., Tauch A., Brinkrolf K.

Unraveling the Chinese hamster ovary cell line transcriptome by next-generation sequencing.

J. Biotechnol. 156:227-235(2011)

 

PubMed=22971049; DOI=10.1021/pr300476w; PMCID=PMC3772721

Baycin-Hizal D., Tabb D.L., Chaerkady R., Chen L., Lewis N.E., Nagarajan H., Sarkaria V., Kumar A., Wolozny D., Colao J., Jacobson E., Tian Y., O'Meally R.N., Krag S.S., Cole R.N., Palsson B.O., Zhang H., Betenbaugh M.J.

Proteomic analysis of Chinese hamster ovary cells.

J. Proteome Res. 11:5265-5276(2012)

 

DOI=10.3390/pr1030296

Wurm F.M.

CHO quasispecies -- implications for manufacturing processes.

Processes 1:296-311(2013)

 

PubMed=23873082; DOI=10.1038/nbt.2624

Lewis N.E., Liu X., Li Y.-X., Nagarajan H., Yerganian G., O'Brien E., Bordbar A., Roth A.M., Rosenbloom J., Bian C., Xie M., Chen W.-B., Li N., Baycin-Hizal D., Latif H., Forster J., Betenbaugh M.J., Famili I., Xu X., Wang J., Palsson B.O.

Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome.

Nat. Biotechnol. 31:759-765(2013)

 

PubMed=26993211; DOI=10.1016/j.jbiotec.2016.03.022

Klanert G., Jadhav V., Shanmukam V., Diendorfer A.B., Karbiener M., Scheideler M., Hernandez-Bort J.A., Grillari J., Hackl M., Borth N.

A signature of 12 microRNAs is robustly associated with growth rate in a variety of CHO cell lines.

J. Biotechnol. 235:150-161(2016)

 

DOI=10.3390/pr5020020

Wurm F.M., Wurm M.J.

Cloning of CHO cells, productivity and genetic stability -- a discussion.

Processes 5:20.1-20.13(2017)

 

PubMed=28544881; DOI=10.1016/j.cels.2017.04.009

Yusufi F.N.K., Lakshmanan M., Ho Y.-S., Loo B.L.-W., Ariyaratne P., Yang Y.-S., Ng S.K., Tan T.R.-M., Yeo H.C., Lim H.L., Ng S.W., Hiu A.-P., Chow C.P., Wan C., Chen S.-W., Teo G., Song G., Chin J.-X., Ruan X.-A., Sung K.W.-K., Hu W.-S., Yap M.G.-S., Bardor M., Nagarajan N., Lee D.-Y.

Mammalian systems biotechnology reveals global cellular adaptations in a recombinant CHO cell line.

Cell Syst. 4:530-542.e6(2017)

 

PubMed=32078082; DOI=10.1007/s10529-020-02841-0

Capella Roca B., Doolan P., Barron N., O'Neill F., Clynes M.

Altered gene expression in CHO cells following polyamine starvation.

Biotechnol. Lett. 42:927-936(2020)

 

PubMed=32619503; DOI=10.1016/j.ymben.2020.06.002

Szeliova D., Ruckerbauer D.E., Galleguillos S.N., Petersen L.B., Natter K., Hanscho M., Troyer C., Causon T., Schoeny H., Christensen H.B., Lee D.-Y., Lewis N.E., Koellensperger G., Hann S., Nielsen L.K., Borth N., Zanghellini J.

What CHO is made of: variations in the biomass composition of Chinese hamster ovary cell lines.

Metab. Eng. 61:288-300(2020)

 

PubMed=34050613; DOI=10.1002/biot.202100165

Wurm M.J., Wurm F.M.

Naming CHO cells for bio-manufacturing: genome plasticity and variant phenotypes of cell populations in bioreactors question the relevance of old names.

Biotechnol. J. 16:e2100165.1-e2100165.13(2021)

 

PubMed=34737324; DOI=10.1038/s41598-021-00779-5; PMCID=PMC8569163

Lung O., Candlish R.C., Nebroski M., Kruckiewicz P., Buchanan C., Moniwa M.

High-throughput sequencing for species authentication and contamination detection of 63 cell lines.

Sci. Rep. 11:21657-21657(2021)

 

PubMed=36610518; DOI=10.1016/j.ymben.2022.12.009; PMCID=PMC11132536

Kotidis P., Donini R., Arnsdorf J., Hansen A.H., Voldborg B.G.R., Chiang A.W.-T., Haslam S.M., Betenbaugh M.J., Jimenez del Val I., Lewis N.E., Krambeck F.J., Kontoravdi C.

CHOGlycoNET: comprehensive glycosylation reaction network for CHO cells.

Metab. Eng. 76:87-96(2023)