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- 2026年01月09日
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T25
COS1/COS1细胞系/COS1细胞株/COS1非洲绿猴肾成纤维细胞
Cell line name COS-1
Synonyms Cos-1; COS 1; Cos 1; COS1; Cos1; CV-1 in Origin Simian-1
Accession CVCL_0223
Resource Identification Initiative To cite this cell line use: COS-1 (RRID:CVCL_0223)
Comments Group: Non-human primate cell line.
Doubling time: ~48 hours (DSMZ=ACC-63).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pSV6-1).
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Species of origin Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops) (NCBI Taxonomy: 9534)
Hierarchy Parent: CVCL_0229 (CV-1)
Children:
CVCL_Z934 (COS-1-InVitrus) CVCL_ZW94 (COS-1-pA104R) CVCL_FA30 (COS-1/B2T.cl.17)
CVCL_A5QA (COS-F2-130) CVCL_C760 (COS-M5) CVCL_8561 (COS-M6)
CVCL_D8FN (Ubigene COS-1 ANO6 KO) CVCL_D8FP (Ubigene COS-1 MFSD8 KO) CVCL_D8FQ (Ubigene COS-1 TMEM184B KO)
Sex of cell Male
Age at sampling Adult
Category Transformed cell line
Publications
PubMed=6260373; DOI=10.1016/0092-8674(81)90282-8
Gluzman Y.
SV40-transformed simian cells support the replication of early SV40 mutants.
Cell 23:175-182(1981)
PubMed=34737324; DOI=10.1038/s41598-021-00779-5; PMCID=PMC8569163
Lung O., Candlish R.C., Nebroski M., Kruckiewicz P., Buchanan C., Moniwa M.
High-throughput sequencing for species authentication and contamination detection of 63 cell lines.
Sci. Rep. 11:21657-21657(2021)
Cross-references
Cell line collections (Providers) AddexBio; T0014001/34
ATCC; CRL-1650
BCRC; 60002
BCRJ; 0070
CLS; 305005
DSMZ; ACC-63
ECACC; 88031701
ICLC; ATL01002
IZSLER; BS CL 95
JCRB; JCRB9082
KCB; KCB 93018YJ
KCLB; 21650
NCBI_Iran; C518
RCB; RCB0143
Ubigene; YC-A031
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文献和实验*发表【中文论文】请标注：由上海酶研生物科技有限公司提供；
*发表【英文论文】请标注：From Shanghai EK-Bioscience Biotechnology Co., Ltd.
Note: This protocol has been optimized for Cos-1 cells. Successful transfection of each cell type requires optimization of the basic protocol. Variables to consider for optimization include, but are not limited to, cell density, transfection
相关专题 总有一种转染方法适合你 Note: This protocol has been optimized for Cos-1 Cell s. Successful transfection of each cell type requires optimization of the basic protocol. Variables to consider for optimization include
问：cos-7的转染1、细胞的培养：我们在培养细胞时发现该细胞长的很快，不到2天的时间就长满了；消化的时候老是成块的掉，如果消化时间加长，细胞也能成单个的，但是细胞的生长力又减弱了，请问要怎样来克服这一问题呢？2、转染前：转染前16-24h我们用含血清不含抗生素的MEM传进6孔盘，16h左右就发现细胞贴壁了，但是形态没有以前好，并且漂死细胞相当多。最初我们都以为是细胞没有贴壁，又把细胞放进培养箱，结果死细胞越来也多，请问是不是因为没有抗生素所以细胞才很容易死，并且形态不好的呢？3、转染：在6孔
技术资料
