LX-2细胞

LX-2/LX-2细胞系/LX-2细胞株/LX-2人肝星形细胞

Cell line name LX-2

Synonyms Lieming Xu-2

Accession CVCL_5792

Resource Identification Initiative To cite this cell line use: LX-2 (RRID:CVCL_5792)

Comments Characteristics: Able to grow under reduced serum conditions (1% FBS) (PubMed=15591520).

Doubling time: ~3-4 days (PubMed=15591520); 53.3 +- 2.3 hours (PubMed=21930125).

Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P03070; SV40 large T antigen.

Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).

Omics: Extracellular matrix proteome analysis.

Omics: Deep quantitative proteome analysis.

Anecdotal: Cell line name is derived from the name of the first author of the original paper.

Derived from site: In situ; Liver; UBERON=UBERON_0002107.

Cell type: Hepatic stellate cell; CL=CL_0000632.

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy Parent: CVCL_5790 (LX-1 [Human HSC])

Children:

CVCL_E1LU (HyCyte LX-2 KO-hNGLY1)

Sex of cell Male

Age at sampling Age unspecified

Category Transformed cell line

STR profile Source(s): Millipore=SCC064; PubMed=24116068; Technion Genomics Center

 

Markers:

Amelogenin X,Y

CSF1PO 10,12

D1S1656 12

D2S441 11,14

D2S1338 17

D3S1358 13,15

D5S818 11,12

D7S820 11

D8S1179 13

D10S1248 13

D12S391 15,17

D13S317 11,13

D16S539 13

D18S51 12

D19S433 13,15.2

D21S11 28,31

D22S1045 16

DYS391 10

FGA 21,26

Penta D 12,13,15

Penta E 5,21

TH01 9.3

TPOX 8,9

vWA 17

 

Run an STR similarity search on this cell line

Publications

PubMed=15591520; DOI=10.1136/gut.2004.042127; PMCID=PMC1774377

Xu L.-M., Hui A.Y., Albanis E., Arthur M.J., O'Byrne S.M., Blaner W.S., Mukherjee P., Friedman S.L., Eng F.J.

Human hepatic stellate cell lines, LX-1 and LX-2: new tools for analysis of hepatic fibrosis.

Gut 54:142-151(2005)

 

PubMed=17760834; DOI=10.1111/j.1582-4934.2007.00060.x; PMCID=PMC3823251

Herrmann J., Gressner A.M., Weiskirchen R.

Immortal hepatic stellate cell lines: useful tools to study hepatic stellate cell biology and function?

J. Cell. Mol. Med. 11:704-722(2007)

 

PubMed=19085953; DOI=10.1002/hep.22697; PMCID=PMC2891538

Guo J.-S., Loke J., Zheng F., Hong F., Yea S., Fukata M., Tarocchi M., Abar O.T., Huang H.-J., Sninsky J.J., Friedman S.L.

Functional linkage of cirrhosis-predictive single nucleotide polymorphisms of Toll-like receptor 4 to hepatic stellate cell responses.

Hepatology 49:960-968(2009)

 

PubMed=21930125; DOI=10.1016/j.yexmp.2011.09.002

Castilho-Fernandes A., de Almeida D.C., Fontes A.M., Melo F.U.F., Picanco-Castro V., Cristina Correa de Freitas M., Orellana M.D., Palma P.V.B., Hackett P.B., Friedman S.L., Covas D.T.

Human hepatic stellate cell line (LX-2) exhibits characteristics of bone marrow-derived mesenchymal stem cells.

Exp. Mol. Pathol. 91:664-672(2011)

 

PubMed=22694338; DOI=10.1021/pr3000927; PMCID=PMC3411196

Rashid S.T., Humphries J.D., Byron A., Dhar A., Askari J.A., Selley J.N., Knight D., Goldin R.D., Thursz M., Humphries M.J.

Proteomic analysis of extracellular matrix from the hepatic stellate cell line LX-2 identifies CYR61 and Wnt-5a as novel constituents of fibrotic liver.

J. Proteome Res. 11:4052-4064(2012)

 

PubMed=24116068; DOI=10.1371/journal.pone.0075692; PMCID=PMC3792989

Weiskirchen R., Weimer J., Meurer S.K., Kron A., Seipel B., Vater I., Arnold N., Siebert R., Xu L.-M., Friedman S.L., Bergmann C.

Genetic characteristics of the human hepatic stellate cell line LX-2.

PLoS ONE 8:E75692-E75692(2013)

 

PubMed=34884585; DOI=10.3390/ijms222312782; PMCID=PMC8657869

Schinagl M., Tomin T., Gindlhuber J., Honeder S., Pfleger R., Schittmayer M., Trauner M., Birner-Gruenberger R.

Proteomic changes of activated hepatic stellate cells.

Int. J. Mol. Sci. 22:12782.1-12782.17(2021)