Jurkat（clone E6-1）细胞

Jurkat,CloneE6-1/Jurkat,CloneE6-1细胞系/Jurkat,CloneE6-1细胞株/Jurkat,CloneE6-1人急性T淋巴细胞白血病细胞

Cell line name Jurkat E6.1

Synonyms JurkatE6-1; Jurkat E6-1; Jurkat, Clone E6-1; Jurkat Clone E6-1; Jurkat (clone E6-1); JURKAT E-6.1; JURKAT E-61; Jurkat-CloneE61; Jurkat-E6; Jurkat E6; J.E6-1; E6-1; Jurkat J6

Accession CVCL_0367

Secondary accession CVCL_4530

Resource Identification Initiative To cite this cell line use: Jurkat E6.1 (RRID:CVCL_0367)

Comments Group: Space-flown cell line (cellonaut).

Part of: Tumor Immunology Bank (TIB) collection from Salk (transferred to ATCC in 1981).

Population: Caucasian.

Doubling time: 20.7 +- 2.2 hours (PubMed=10468210).

Omics: Cell surface proteome.

Omics: Deep phosphoproteome analysis.

Omics: Deep quantitative phosphoproteome analysis.

Omics: Genome sequenced.

Omics: H3K4me3 ChIP-seq epigenome analysis.

Omics: SNP array analysis.

Omics: Transcriptome analysis by RNAseq.

Anecdotal: Have been flown in space on shuttle flights STS-76, STS-80 and STS-95 to study apoptosis and cytoskeleton modifications (PubMed=9707173; PubMed=10832058; PubMed=11481229; PubMed=11669127).

Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.

Cell type: T-cell; CL=CL_0000084.

Sequence variations

Mutation; HGNC; 959; BAX; Simple; p.Glu41Argfs*19 (c.121delG); ClinVar=VCV000009512; Zygosity=Heterozygous (PubMed=29739316).

Mutation; HGNC; 959; BAX; Simple; p.Glu41Glyfs*33 (c.121dupG); ClinVar=VCV000009511; Zygosity=Heterozygous (PubMed=29739316).

Mutation; HGNC; 16712; FBXW7; Simple; p.Arg505Cys (c.1513C>T); ClinVar=VCV000069961; Zygosity=Heterozygous (from parent cell line).

Mutation; HGNC; 6079; ID; Simple; p.Gln345Ter (c.1033C>T); Zygosity=Heterozygous (PubMed=29739316).

Mutation; HGNC; 607; Simple; c.1097+1065_1097+1112del47; Zygosity=Heterozygous (PubMed=29739316).

Mutation; HGNC; 7325; MSH2; Simple; p.Arg711Ter (c.2131C>T); ClinVar=VCV000090903; Zygosity=Homozygous (PubMed=29739316).

Mutation; HGNC; 7329; MSH6; Simple; p.Phe1088Serfs*2 (c.3261delC); ClinVar=VCV000089363; Zygosity=Homozygous (from parent cell line).

Mutation; HGNC; 11491; SYK; Simple; p.Met34Hisfs*3 (c.98_99insG); Zygosity=Heterozygous (PubMed=7592872; PubMed=29739316).

Mutation; HGNC; 11998; TP53; Simple; p.Arg196Ter (c.586C>T); ClinVar=VCV000043589; Zygosity=Heterozygous (PubMed=29739316).

HLA typing Source: PubMed=26589293

Class I

HLA-A A*03:01,03:01

HLA-B B*07:02,35:03

HLA-C C*04:01,07:02

Disease Childhood T acute lymphoblastic leukemia (NCIt: C7953)

Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861)

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

PubMed=11669127; DOI=10.1016/S0094-5765(01)00116-3

Schatten H., Lewis M.L., Chakrabarti A.

Spaceflight and clinorotation cause cytoskeleton and mitochondria changes and increases in apoptosis in cultured cells.

Acta Astronaut. 49:399-418(2001)

 

PubMed=15472075; DOI=10.1126/science.1102160

Weng A.P., Ferrando A.A., Lee W., Morris J.P. 4th, Silverman L.B., Sanchez-Irizarry C., Blacklow S.C., Look A.T., Aster J.C.

Activating mutations of NOTCH1 in human T cell acute lymphoblastic leukemia.

Science 306:269-271(2004)

 

PubMed=18842727; DOI=10.1128/JVI.01726-08; PMCID=PMC2593302

Takeuchi Y., McClure M.O., Pizzato M.

Identification of gammaretroviruses constitutively released from cell lines used fo\irus research.

J. Virol. 82:12585-12588(2008)

 

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662

Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Cancer Res. 70:2158-2164(2010)

 

PubMed=23933134; DOI=10.1016/j.jprot.2013.07.019

Storvold G.L., Landskron J., Strozynski M., Arntzen M.O., Koehler C.J., Kalland M.E., Tasken K., Thiede B.

Quantitative profiling of tyrosine phosphorylation revealed changes in the activity of the T cell receptor signaling pathway upon cisplatin-induced apoptosis.

J. Proteomics 91:344-357(2013)

 

PubMed=25485619; DOI=10.1038/nbt.3080

Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.

A comprehensive transcriptional portrait of human cancer cell lines.

Nat. Biotechnol. 33:306-312(2015)

 

PubMed=25877200; DOI=10.1038/nature14397

Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.

A resource for cell line authentication, annotation and quality control.

Nature 520:307-311(2015)

 

PubMed=25894527; DOI=10.1371/journal.pone.0121314; PMCID=PMC4404347

Bausch-Fluck D., Hofmann A., Bock T., Frei A.P., Cerciello F., Jacobs A., Moest H., Omasits U., Gundry R.L., Yoon C., Schiess R., Schmidt A., Mirkowska P., Hartlova A.S., Van Eyk J.E., Bourquin J.-P., Aebersold R., Boheler K.R., Zandstra P.W., Wollscheid B.

A mass spectrometric-derived cell surface protein atlas.

PLoS ONE 10:E0121314-E0121314(2015)

 

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878

Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Genome Med. 7:118.1-118.7(2015)

 

PubMed=26546556; DOI=10.1016/j.jprot.2015.10.029

Nguyen T.D., Carrascal M., Vidal-Cortes O., Gallardo O., Casas V., Gay M., Phan V.C., Abian J.

The phosphoproteome of human Jurkat T cell clones upon costimulation with anti-CD3/anti-CD28 antibodies.

J. Proteomics 131:190-198(2016)

 

PubMed=28464451; DOI=10.1002/pmic.201600470

Tran T.T., Strozynski M., Thiede B.

Quantitative phosphoproteome analysis of cisplatin-induced apoptosis in Jurkat T cells.

Proteomics 17:1600470.1-1600470.11(2017)

 

PubMed=29739316; DOI=10.1186/s12864-018-4718-6; PMCID=PMC5941560

Gioia L., Siddique A., Head S.R., Salomon D.R., Su A.I.

A genome-wide survey of mutations in the Jurkat cell line.

BMC Genomics 19:334.1-334.13(2018)

 

PubMed=32938764; DOI=10.1128/JVI.01334-20; PMCID=PMC7654282

Fernandez M.V., Hoffman H.K., Pezeshkian N., Tedbury P.R., van Engelenburg S.B., Freed E.O.

Elucidating the basis for permissivity of the MT-4 T-cell line to replication of an HIV-1 mutant lacking the gp41 cytoplasmic tail.

J. Virol. 94:e01334.20.1-e01334.20.27(2020)