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RNA Polymerases I/II/III Subun

it ABC1 Rabbit Monoclonal Antibody
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  • ¥1350 - 2250
  • EnkiLife
  • AMRe02457
  • 2025年07月11日
  • WB;IHC
  • Rabbit
  • Human;Mouse;Rat
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  • 企业认证

    • 详细信息
    • 文献和实验
    • 技术资料
    • 标记物

      Unconjugated

    • 适应物种

      Human;Mouse;Rat

    • 供应商

      武汉恩玑生命科技有限公司

    • 宿主

      Rabbit

    • 应用范围

      WB;IHC

    • 规格

      50uL/100uL

    规格:50uL产品价格:¥1350.0
    规格:100uL产品价格:¥2250.0

    产品概述

    产品名称(Product Name)

    RNA Polymerases I/II/III Subunit ABC1 Rabbit Monoclonal Antibody

    描述(Description)

    Recombinant Rabbit Monoclonal antibody

    宿主(Host)

    Rabbit

    应用(Application)

    WB,IHC-P

    种属反应性(Reactivity)

    Human,Mouse,Rat

     

    产品性能

    偶联物(Conjugation)

    Unconjugated

    修饰(Modification)

    Unmodified

    同种型(Isotype)

    IgG

    克隆(Clonality)

    Monoclonal Antibody

    形式(Form)

    Liquid

    存放说明(Storage)

    Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

    储存溶液(Buffer)

    50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

    纯化方式(Purification)

    Affinity Purified

     

    免疫原

    基因名(Gene Name)

    POLR2E

    别名(Alternative Names)

    RPB5; XAP4; RPABC1; hRPB25; hsRPB5

    基因ID(Gene ID)

    5434

    蛋白ID(SwissProt ID)

    P19388

     

    产品应用

    稀释比(Dilution Ratio)

    WB: 1/500-1/1000 IHC: 1/50-1/100

    蛋白分子量(Molecular Weight)

    Calculated MW: 25 kDa; Observed MW: 25 kDa

     

    研究背景

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process .

     

    研究领域

    Epigenetics and Nuclear Signaling

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    图标文献和实验
    相关实验
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      antibody unconjugated; 货号21337,产品描述:GAPDH Antibody;规格 50 ul / 100 ul; 货号3052,产品描述:Rabbit anti-Human IgG secondary antibody HRP conjugated; 货号300904050,产品描述:One-Hour Western kit,10 ml / bottle x 3;

    • secondary antibody review -- data from 99 publications

      cytometry   used as a control to detect cell responses targeted antigen   7       Alexa Fluor 488         7       Cy3         8 goat IgG   Alexa Fluor 488   1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey

    • Native Chromatin Preparation and Illumina/Solexa Library Construction

      Antibody and preimmune serum for ChIP CD4+ T cell Isolation Kit II (human) (Miltenyi Biotec) Alternatively, Dynabeads Untouched Human CD4 T Cells Kit (Invitrogen) can be used (see Steps 5-21). DNA End-Repair Kit (Epicentre Biotechnologies

    图标技术资料

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    RNA Polymerases I/II/III Subunit ABC1 Rabbit Monoclonal Antibody
    ¥1350 - 2250