- ￥1200 - 2150
- EnkiLife
- AMM03615
- 2025年07月14日
- WB;IHC
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 标记物：
Unconjugated
- 适应物种：
Human
- 供应商：
武汉恩玑生命科技有限公司
- 宿主：
Mouse
- 应用范围：
WB;IHC
- 规格：
50uL/100uL
| 规格： | 50uL | 产品价格： | ￥1200.0 |
|---|---|---|---|
| 规格： | 100uL | 产品价格： | ￥2150.0 |
产品概述
产品性能
免疫原
产品应用
研究背景
Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels. In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway.
研究领域
Signal Transduction
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
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