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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Human IgG3 myeloma protein
- 保存条件:
2-8°C
- 保质期:
2年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
小鼠
- 应用范围:
ELISA, ELISPOT, IHC-FS, IHC-PS, WB
- 抗体英文名:
Mouse Anti-Human IgG3 Hinge-AP
- 抗体名:
Mouse Anti-Human IgG3 Hinge-AP
小鼠抗人IgG3铰链-AP,Mouse Anti-Human IgG3 Hinge-AP,小鼠抗人IgG3铰链-AP,Mouse Anti-Human IgG3 Hinge-AP
产品名称:小鼠抗人IgG3铰链-AP-Mouse Anti-Human IgG3 Hinge-AP
产品货号:9210-04
产品规格:1.0mL

应用类型:ELISA, ELISPOT, IHC-FS, IHC-PS, WB

用于ELISA、免疫组织化学、蛋白质印迹和ELISpot测定的AP抗人IgG3铰链抗体。

小鼠抗人IgG3铰链-AP-Mouse Anti-Human IgG3 Hinge-AP2-8°C
点击:小鼠抗人IgG3铰链-AP-Mouse Anti-Human IgG3 Hinge-AP更多SouthernBiotech产品信息价格,货期,产品说明,百度搜“艾美捷科技”进入官网网站,查看具体产品且快速下单。

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文献和实验which employs goat anti-Rabbit HRP.12. Wash filter 3 times 7 minutes with 0.5% BSA in TBST.13. Perform Alkaline phosphatase reaction using NBT and BCIP substrates. I find that CREB usually comes up within several minutes of adding the AP substrates
Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
Materials Anti-digoxigenin-AP conjugate (Roche #11093274910, 150U/200 uL, $168) Levamisole (Sigma #T1512, 2 g, $10) (blocks endogenous phosphatase activity) BCIP (Roche #11383221001, 3 mL, $49) NBT (Roche #11383213001, 3 mL, $48)
% nonfat dry milk works well for anti-PCREB, but in my hands milk is not as good as BSA from USB (it seems to mask the epitope). Remove block solution and add primary antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000
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