- ￥600 - 3200
- 上海莼试
- CS-01Y65541
- 国产
- 2025年07月15日
8 年钻石会员
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 库存：
21
- 英文名：
BI6727(Volasertib)
- CAS号：
755038-65-4
- 供应商：
上海莼试
- 保存条件：
4°C, protect from light
- 规格：
10mM (in 1mL DMSO) 5mg 10mg 50mg 100mg
商品属性：
产品描述：
分子式:C34H50N8O3
分子量:618.83
溶解度:≥ 10.3mg/mL in DMSO
储存条件:4°C, protect from light
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
Polo-like kinase 1 (Plk1) is an early trigger for G2/M phase transition and is over-expressed in a variety of cancers for that being regarded as a promising target for cancer treatment [1].BI6727 (Volasertib) is a potent Plk1 inhibitor and is regarded as a promising drug for multiple cancers in clinic. When tested with NB TICs and normal human pediatric SKPs (neural crest-like stem cells), BI6727 (Volasertib) treatment inhibits NB TICs with EC50 value of 21 nM/L and 2.8 μM/L on SKPs and decresed TIC survival [2]. It has been reported that BI6727 (Volasertib) inhibited proliferation of multiple cell lines, including HCT 116(caicinomas of the colon), NCI-H460 (lung cancer), BRO (melanoma), GRANTA (hematopoitic cancers) with EC50 value of 23 nmol/l, 21 nmol/l, 11 nmol/l, 15 nmol/l, respectively [1] [3].In nude mice model with HCT 16 cells (colon carcinoma) subcutaneous xenograft, oral administration of BI6727 (Volasertib) delays tumor growth, decreased tumor size and induced tumor regression by increasing the mitotic index and apoptosis. And the same results were achieved when tested with NCI-H4660 (non-small cell lung carcinoma), xenograft model [1].References:[1]. Rudolph, D., et al., BI 6727, a Polo-like kinase inhibitor with improved pharmacokinetic profile and broad antitumor activity. Clin Cancer Res, 2009. 15(9): p. 3094-102.[2]. Grinshtein, N., et al., Small molecule kinase inhibitor screen identifies polo-like kinase 1 as a target for neuroblastoma tumor-initiating cells. Cancer Res, 2011. 71(4): p. 1385-95.[3]. Munch, C., et al., Therapeutic polo-like kinase 1 inhibition results in mitotic arrest and suquent cell death of blasts in the bone marrow of AML patients and has similar effects in non-neoplastic cell lines. Leuk Res, 2015. 39(4): p. 462-70.
注意事项：
抗逆滴加序列
每次向板内滴加抗原时，移液器滴头要与平面45度悬空，不要触碰到孔内的液体，由后向前依次滴加(即浓度由低往高滴加)。
抗感染反应期
抗原抗体在室温20~25℃下，必须反应30min以上，若环境温度低于室温，可将微量反应板置于恒温培养箱中，使二者充分反应。
旅游温度
磷酸盐缓冲液的pH值要在高压灭菌后进行滴定，往往在高压后pH值会有所改变，所以高压后再调一次pH值更为准确。磷酸盐缓冲液一经使用保存期不要超过3周。当pH<5.8时，红细胞会产生自凝现象;当pH>7.8时，图形洗脱加快，易造成肉眼观察产生误差;p H=7.2时，红细胞沉降最充分，图形最清晰。
当滴注 1%红细胞悬液时，应经常摇晃红细胞悬液，使红细胞均匀地分布在磷酸缓冲液中，以防止红细胞下降。
反应时间及温度
加入鸡红血球之后，反应板在室温(20~25℃)静置30~40min，对照孔血球下沉于孔底，即可判定结果。若室温达不到实验要求，需相应调整反应时间。当环境温度低于4℃时，红细胞发生自凝;高于37℃时，会发生反应物分离和红细胞溶血。
使用方法：
1.本蛋白酶抑制剂混合物为100×的储存液，使用时按照1:100的比例加入到裂解液中(例如，1ml裂解液中加入10μl蛋白酶抑制剂混合物)，混匀后即可使用。根据需要，0.5M的EDTA也按照1:100的比例加入到裂解液中（如用于检测金属蛋白酶活性，则不宜添加EDTA）。含有蛋白酶抑制剂混合物的裂解液宜现用现配，不宜配制后冻存待后续使用。
2. 待所需的抑制剂添加完毕混匀后，就可以开始进行哺乳动物组织的裂解和蛋白提取。
公司正在出售的产品：
| 货号 | CS-01Y65541 | 规格 | 10mM (in 1mL DMSO) 5mg 10mg 50mg 100mg |
| CAS号 | 755038-65-4 | 分子量 | 618.83 |
| 含量 | >98.00% | 别名 | BI 6727; BI-6727 |
| 分子式 | C34H50N8O3 | 化学名 | |
| 产地 | 国产 | 用途 | 仅供科研研究实验 |
分子式:C34H50N8O3
分子量:618.83
溶解度:≥ 10.3mg/mL in DMSO
储存条件:4°C, protect from light
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
Polo-like kinase 1 (Plk1) is an early trigger for G2/M phase transition and is over-expressed in a variety of cancers for that being regarded as a promising target for cancer treatment [1].BI6727 (Volasertib) is a potent Plk1 inhibitor and is regarded as a promising drug for multiple cancers in clinic. When tested with NB TICs and normal human pediatric SKPs (neural crest-like stem cells), BI6727 (Volasertib) treatment inhibits NB TICs with EC50 value of 21 nM/L and 2.8 μM/L on SKPs and decresed TIC survival [2]. It has been reported that BI6727 (Volasertib) inhibited proliferation of multiple cell lines, including HCT 116(caicinomas of the colon), NCI-H460 (lung cancer), BRO (melanoma), GRANTA (hematopoitic cancers) with EC50 value of 23 nmol/l, 21 nmol/l, 11 nmol/l, 15 nmol/l, respectively [1] [3].In nude mice model with HCT 16 cells (colon carcinoma) subcutaneous xenograft, oral administration of BI6727 (Volasertib) delays tumor growth, decreased tumor size and induced tumor regression by increasing the mitotic index and apoptosis. And the same results were achieved when tested with NCI-H4660 (non-small cell lung carcinoma), xenograft model [1].References:[1]. Rudolph, D., et al., BI 6727, a Polo-like kinase inhibitor with improved pharmacokinetic profile and broad antitumor activity. Clin Cancer Res, 2009. 15(9): p. 3094-102.[2]. Grinshtein, N., et al., Small molecule kinase inhibitor screen identifies polo-like kinase 1 as a target for neuroblastoma tumor-initiating cells. Cancer Res, 2011. 71(4): p. 1385-95.[3]. Munch, C., et al., Therapeutic polo-like kinase 1 inhibition results in mitotic arrest and suquent cell death of blasts in the bone marrow of AML patients and has similar effects in non-neoplastic cell lines. Leuk Res, 2015. 39(4): p. 462-70.
注意事项：
抗逆滴加序列
每次向板内滴加抗原时，移液器滴头要与平面45度悬空，不要触碰到孔内的液体，由后向前依次滴加(即浓度由低往高滴加)。
抗感染反应期
抗原抗体在室温20~25℃下，必须反应30min以上，若环境温度低于室温，可将微量反应板置于恒温培养箱中，使二者充分反应。
旅游温度
磷酸盐缓冲液的pH值要在高压灭菌后进行滴定，往往在高压后pH值会有所改变，所以高压后再调一次pH值更为准确。磷酸盐缓冲液一经使用保存期不要超过3周。当pH<5.8时，红细胞会产生自凝现象;当pH>7.8时，图形洗脱加快，易造成肉眼观察产生误差;p H=7.2时，红细胞沉降最充分，图形最清晰。
当滴注 1%红细胞悬液时，应经常摇晃红细胞悬液，使红细胞均匀地分布在磷酸缓冲液中，以防止红细胞下降。
反应时间及温度
加入鸡红血球之后，反应板在室温(20~25℃)静置30~40min，对照孔血球下沉于孔底，即可判定结果。若室温达不到实验要求，需相应调整反应时间。当环境温度低于4℃时，红细胞发生自凝;高于37℃时，会发生反应物分离和红细胞溶血。
使用方法：
1.本蛋白酶抑制剂混合物为100×的储存液，使用时按照1:100的比例加入到裂解液中(例如，1ml裂解液中加入10μl蛋白酶抑制剂混合物)，混匀后即可使用。根据需要，0.5M的EDTA也按照1:100的比例加入到裂解液中（如用于检测金属蛋白酶活性，则不宜添加EDTA）。含有蛋白酶抑制剂混合物的裂解液宜现用现配，不宜配制后冻存待后续使用。
2. 待所需的抑制剂添加完毕混匀后，就可以开始进行哺乳动物组织的裂解和蛋白提取。
公司正在出售的产品：
| Recombnant Human ATG4A | Alexa Fluor 750标记的羊抗鸡IgG H&L |
| 碱性磷酸酶（AP）标记的羊抗大鼠IgG H&L | Rabbit Anti-Human IgG H&L / AF647 |
| FITC标记的兔抗牛IgG H&L | Rabbit Anti-Human IgG H&L / Gold |
| PE-Cy7标记的兔抗小鼠IgG H&L | Rabbit Anti-Human IgA / Biotin |
| PE-Cy7标记的鼠抗人IgG H&L单克隆抗体 | Rabbit Anti-Goat IgG H&L / APC |
| Alexa Fluor 405标记的羊抗鸡IgG H&L | Goat Anti-Mouse IgG H&L / APC |
| PE-CY5标记的兔抗猴IgG H&L | Rabbit Anti-Human IgG H&L / PE-Cy7 |
| 碱性磷酸酶（AP）标记的羊抗人IgM | HV tag多肽 |
| Alexa Fluor 647标记的兔抗人IgG H&L | Goat Anti-Human IgM / AP |
| 胶体金标记的兔抗人IgG H&L | Rabbit Anti-Monkey IgG H&L / PE-Cy5 |
| APC标记的兔抗羊IgG H&L | Goat Anti-Chicken IgG H&L / AF405 |
| 生物素标记的兔抗人IgA | Mouse Anti-Human IgG H&L (3D3) mAb / PE-Cy7 |
| APC标记的羊抗小鼠IgG H&L | BI6727(Volasertib)Rabbit Anti-Mouse IgG H&L / PE-Cy7 |
| PE-Cy7标记的兔抗人IgG H&L | Rabbit Anti-Bovine IgG H&L / FITC |
| Goat Anti-Chicken IgG H&L / AF750 | Goat Anti-Rat IgG H&L / AP |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料
BI6727(Volasertib)
￥600 - 3200
